• British Pharmacopoeia Volume V
  • Appendices
  • Appendix I A. General Reagents

Aminomethylalizarindiacetic Acid Reagent

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Aminomethylalizarindiacetic Acid Reagent

Solution i Dissolve 0.36 g of cerium(iii) nitrate in sufficient water to produce 50 mL.

Solution ii Suspend 0.7 g of 3-aminomethylalizarin-N,N- diacetic acid in 50 mL of water. Dissolve with the aid of about 0.25 mL of 13.5m ammonia, add 0.25 mL of glacial acetic acid and dilute to 100 mL with water.

Solution iii Dissolve 6 g of sodium acetate in 50 mL of water, add 11.5 mL of glacial acetic acid and dilute to 100 mL with water.

To 33 mL of acetone add 6.8 mL of solution iii, 1.0 mL of solution ii and 1.0 mL of solution i and dilute to 50 mL with water. Use within 5 days.

Complies with the following test.

Sensitivity To 1.0 mL of fluoride standard solution (10 ppm F) add 19.0 mL of water and 5.0 mL of the reagent being examined. After 20 minutes, a distinct blue colour is produced.