Desoxycorticosterone Acetate

C23H32O4

372.50

Pregn-4-ene-3,20-dione, 21-(acetyloxy)-.
11-Deoxycorticosterone acetate

[56-47-3].

» Desoxycorticosterone Acetate contains not less than 97.0 percent and not more than 103.0 percent of C23H32O4, calculated on the dried basis.

Packaging and storage— Preserve in well-closed, light-resistant containers. Store at 25

, excursions permitted between 15

and 30

USP Reference standards

—
USP Desoxycorticosterone Acetate RS.

Identification—

A: Infrared Absorption

197K

B: Ultraviolet Absorption

197U

—

Solution: 10 µg per mL.

Medium: alcohol.

Absorptivities at 240 nm, calculated on the dried basis, do not differ by more than 3.0%.

Melting range

741

: between 155

and 161

Specific rotation

781S

: between +171

and +179

Test solution: 10 mg, undried, per mL, in dioxane.

Loss on drying

731

— Dry it in vacuum over silica gel for 4 hours: it loses not more than 0.5% of its weight.

Assay—

Standard preparation— Prepare as directed for Standard Preparation under Assay for Steroids

351

, using USP Desoxycorticosterone Acetate RS.

Assay preparation— Accurately weigh about 100 mg of Desoxycorticosterone Acetate, dissolve in sufficient alcohol to make 200.0 mL, and mix. Pipet 5 mL of this solution into a 250-mL volumetric flask, add alcohol to volume, and mix. Pipet 20 mL of the resulting solution into a glass-stoppered, 50-mL conical flask.

Procedure— Proceed as directed for Procedure under Assay for Steroids

351

. Calculate the quantity, in mg, of C23H32O4 in the Desoxycorticosterone Acetate taken by the formula:

10C(AU / AS).

Auxiliary Information— Please check for your question in the FAQs before contacting USP.

Topic/Question	Contact	Expert Committee
Monograph	Daniel K. Bempong, Ph.D. Senior Scientist 1-301-816-8143	(MDPS05) Monograph Development-Pulmonary and Steroids
Reference Standards	Lili Wang, Technical Services Scientist 1-301-816-8129 RSTech@usp.org

USP32–NF27 Page 2081

Pharmacopeial Forum: Volume No. 29(5) Page 1456