Edetic Acid
Glycine, N,N¢-1,2-ethanediylbis[N-(carboxymethyl)-. (Ethylenedinitrilo)tetraacetic acid ![]() ![]() ![]() » Edetic Acid contains not less than 98.0 percent and not more than 100.5 percent of C10H16N2O8.
Packaging and storage—
Preserve in well-closed containers.
Identification, Infrared Absorption
![]() ![]()
Residue on ignition
![]() ![]()
Heavy metals, Method II
![]() ![]()
Iron—
Char 3.0 g thoroughly, and heat in an oven at 500
![]() ![]()
Limit of nitrilotriacetic acid—
Mobile phase
, Cupric nitrate solution, Stock standard solution, and Chromatographic system—Prepare as directed in the test for Limit of nitrilotriacetic acid under Edetate Disodium.
Resolution solution—
Using Edetic Acid instead of Edetate Disodium, prepare as directed for Resolution solution in the test for Limit of nitrilotriacetic acid under Edetate Disodium.
Standard preparation—
Transfer 1.0 g of Edetic Acid to a 100-mL volumetric flask, add 300 µL of Stock standard solution, dilute with Cupric nitrate solution to volume, and mix. Sonicate, if necessary, to achieve complete solution.
Test preparation—
Transfer 1.0 g of Edetic Acid to a 100-mL volumetric flask, dilute with Cupric nitrate solution to volume, and mix. Sonicate, if necessary, to achieve complete solution.
Procedure—
Proceed as directed for Procedure in the test for Limit of nitrilotriacetic acid under Edetate Disodium: the response of the nitrilotriacetic acid peak of the Test preparation does not exceed the difference between the nitrilotriacetic acid peak responses obtained from the Standard preparation and the Test preparation (0.3%).
Assay—
Assay preparation—
Transfer about 1.4 g of Edetic Acid, accurately weighed, to a 100-mL volumetric flask, dissolve in 11 mL of 1 N sodium hydroxide, dilute with water to volume, with cooling, if necessary, and mix.
Procedure—
Transfer to a 400-mL beaker about 200 mg of chelometric standard calcium carbonate, previously dried at 110
![]() (292.24 / 100.09)(0.1W / V)
in which 292.24 and 100.09 are the molecular weights of edetic acid and calcium carbonate, respectively, W is the weight, in mg, of calcium carbonate, and V is the volume, in mL, of the Assay preparation consumed in the titration.
Auxiliary Information—
Please check for your question in the FAQs before contacting USP.
Chromatographic Column—
USP32–NF27 Page 1230
Chromatographic columns text is not derived from, and not part of, USP 32 or NF 27.
|