Ethylenediamine
60.101,2-Ethanediamine.
Ethylenediamine
[107-15-3].» Ethylenediamine contains not less than 98.0 percent and not more than 100.5 percent, by weight, of C2H8N2.
note—Ethylenediamine is strongly alkaline and may readily absorb carbon dioxide from the air to form a nonvolatile carbonate. Protect Ethylenediamine against undue exposure to the atmosphere.
Packaging and storage—
Preserve in well-filled, tight, glass containers.
Identification—
To 2 mL of cupric sulfate solution (1 in 100) add 3 drops of a solution of Ethylenediamine (1 in 6), and shake: a purplish blue color is produced.
Heavy metals, Method I 
231
—
Evaporate 5.0 mL on a steam bath to dryness, add to the residue 1 mL of hydrochloric acid and 0.5 mL of nitric acid, and evaporate again to dryness. Dissolve the residue in 20 mL of warm water, cool, dilute with water to 100 mL, mix, and use 20 mL of this solution for the test: the limit is 0.002%.
231—
Evaporate 5.0 mL on a steam bath to dryness, add to the residue 1 mL of hydrochloric acid and 0.5 mL of nitric acid, and evaporate again to dryness. Dissolve the residue in 20 mL of warm water, cool, dilute with water to 100 mL, mix, and use 20 mL of this solution for the test: the limit is 0.002%.
Assay—
Weigh accurately about 1 mL of Ethylenediamine in a tared, glass-stoppered flask containing about 25 mL of water. Dilute with water to about 75 mL, add a mixed indicator of bromocresol green TS and methyl red TS (5 in 6), mix, and titrate with 1 N hydrochloric acid VS. Perform a blank determination, and make any necessary correction. Each mL of 1 N hydrochloric acid is equivalent to 30.05 mg of C2H8N2.
Auxiliary Information—
Please check for your question in the FAQs before contacting USP.
Chromatographic Column—
| Topic/Question | Contact | Expert Committee |
| Monograph | Elena Gonikberg, Ph.D.
Senior Scientist 1-301-816-8251 |
(MDGRE05) Monograph Development-Gastrointestinal Renal and Endocrine |
USP32–NF27 Page 2330
Pharmacopeial Forum: Volume No. 28(6) Page 1789
Chromatographic columns text is not derived from, and not part of, USP 32 or NF 27.