Maltose
» Maltose is a sugar. It contains one molecule of water of hydration or is anhydrous. It contains not less than 92.0 percent of maltose, calculated on the anhydrous basis. The amounts of other sugars, if detected, are not included in the requirements or the calculated amount under Other Impurities.
Packaging and storage
Preserve in well-closed containers. No storage requirements specified.
Identification
A:
Add 2 to 3 drops of a solution of Maltose (1 in 20) to 5 mL of hot alkaline cupric tartrate TS. A red precipitate is formed.
B:
The retention time of the major peak in the chromatogram of the Assay preparation corresponds to that in the chromatogram of the Standard preparation, as obtained in the Assay.
pH
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Water, Method I
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Residue on ignition
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Heavy metals, Method I
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Dextrin, starch, and sulfite
Dissolve 1.0 g of Maltose in 10 mL of water, and add 1 drop of iodine TS: a yellow color develops. Then add 1 drop of starch TS to this portion: a blue color develops.
Assay
Mobile phase
Use degassed water.
Resolution solution
Dissolve accurately weighed quantities of maltotriose, maltose, and glucose in water to obtain a solution having concentrations of about 10 mg of each per g.
Standard preparation
Dissolve an accurately weighed quantity of USP Maltose Monohydrate RS in water to obtain a solution having a concentration of about 10 mg per g. Calculate the exact concentration on the anhydrous basis.
Assay preparation
Dissolve about 0.10 g of Maltose, accurately weighed, in water, and dilute with water to about 10 g. Accurately record the final solution weight, and mix thoroughly.
Chromatographic system (see Chromatography
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Procedure
Separately inject equal volumes (about 20 µL) of the Standard preparation and the Assay preparation into the chromatograph, record the chromatograms, and measure the responses for the major peaks. Calculate the percentage of Maltose on the anhydrous basis, taken by the formula:
[10,000(CS / CU)(rU / rS)]/(100 W)
in which CS is the concentration, in mg per g, on the anhydrous basis of USP Maltose Monohydrate RS in the Standard preparation; CU is the concentration, in mg per g, of Maltose in the Assay preparation; rU and rS are the peak responses obtained from the Assay preparation and the Standard preparation, respectively; and W is the percentage obtained in the test for Water.
Auxiliary Information
Please check for your question in the FAQs before contacting USP.
Chromatographic Column
USP32NF27 Page 1278
Pharmacopeial Forum: Volume No. 31(3) Page 815
Chromatographic columns text is not derived from, and not part of, USP 32 or NF 27.
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