Methacholine Chloride
1-Propanaminium, 2-(acetyloxy)-N,N,N-trimethyl-, chloride, (±)-. (±)-(2-Hydroxypropyl)trimethylammonium chloride acetate ![]() ![]() ![]() » Methacholine Chloride, dried at 105
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Packaging and storage
Preserve in tight containers.
Identification
A:
Dissolve about 100 mg in about 2 mL of water on a watch glass, and add 3 mL of platinic chloride TS: small rhombohedric plates are formed, which melt between 220
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B:
To 1 mL of a solution (1 in 10) add 1 mL of alcohol and 1 mL of sulfuric acid, and heat gently: the odor of ethyl acetate is perceptible.
C:
To 5 mL of a solution (1 in 10) add 2 g of potassium hydroxide and heat gently: the odor of trimethylamine is perceptible.
D:
A solution (1 in 50) responds to the tests for Chloride
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Melting range
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Loss on drying
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Residue on ignition
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Acetylcholine chloride
To 2 mL of a solution (1 in 10) add 3 mL of a solution of sodium perchlorate (1 in 5), shake, and immerse in ice water for 5 minutes: no precipitate is formed.
Heavy metals, Method II
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Assay
Transfer to a conical flask about 400 mg of Methacholine Chloride, previously dried and accurately weighed (because it is very hygroscopic, store the dried material in a vacuum desiccator), dissolve it in 50 mL of glacial acetic acid, add 10 mL of mercuric acetate TS and 1 drop of crystal violet TS, and titrate with 0.1 N perchloric acid VS to a blue-green endpoint. Perform a blank determination, and make any necessary correction. Each mL of 0.1 N perchloric acid is equivalent to 19.57 mg of C8H18ClNO2.
Auxiliary Information
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USP32NF27 Page 2912
Pharmacopeial Forum: Volume No. 34(3) Page 629
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