Neostigmine Bromide
303.20Benzenaminium, 3-[[(dimethylamino)carbonyl]oxy]-N,N,N-trimethyl-, bromide.
(m-Hydroxyphenyl)trimethylammonium bromide dimethylcarbamate
[114-80-7].» Neostigmine Bromide contains not less than 98.0 percent and not more than 102.0 percent of C12H19BrN2O2, calculated on the dried basis.
Packaging and storage—
Preserve in tight containers.
Identification—
B:
A solution (1 in 50) responds to the tests for Bromide 
191
.
191.
Loss on drying 731—
Dry it at 105
for 3 hours: it loses not more than 2.0% of its weight.
731—
Dry it at 105 for 3 hours: it loses not more than 2.0% of its weight.
Residue on ignition 281:
not more than 0.15%.
281:
not more than 0.15%.
Sulfate—
Dissolve 250 mg in 10 mL of water, and add 1 mL of 3 N hydrochloric acid and 1 mL of barium chloride TS: no turbidity is produced immediately.
Assay—
Dissolve about 750 mg of Neostigmine Bromide, accurately weighed, in a mixture of 70 mL of glacial acetic acid and 20 mL of mercuric acetate TS, add 4 drops of crystal violet TS, and titrate with 0.1 N perchloric acid VS to a blue endpoint. Perform a blank determination, and make any necessary correction. Each mL of 0.1 N perchloric acid is equivalent to 30.32 mg of C12H19BrN2O2.
Auxiliary Information—
Please check for your question in the FAQs before contacting USP.
| Topic/Question | Contact | Expert Committee |
| Monograph | Ravi Ravichandran, Ph.D.
Senior Scientist 1-301-816-8330 |
(MDPP05) Monograph Development-Psychiatrics and Psychoactives |
| Reference Standards | Lili Wang, Technical Services Scientist 1-301-816-8129 RSTech@usp.org |
USP32–NF27 Page 3069