Procarbazine Hydrochloride
257.76Benzamide, N-(1-methylethyl)-4-[(2-methylhydrazino)methyl]-, monohydrochloride.
N-Isopropyl-
-(2-methylhydrazino)-p-toluamide monohydrochloride
[366-70-1].» Procarbazine Hydrochloride contains not less than 98.5 percent and not more than 100.5 percent of C12H19N3O·HCl.
Caution: Handle Procarbazine Hydrochloride with exceptional care, since it is a highly potent agent.
Packaging and storage—
Preserve in tight, light-resistant containers.
Identification—
B:
Ultraviolet Absorption 
197U
—[note—Use low-actinic glassware throughout this test.]
197U—[note—Use low-actinic glassware throughout this test.]
Solution:
10 µg per mL.
Medium:
0.1 N hydrochloric acid.
Absorptivities at 232 nm do not differ by more than 3.0%.
Residue on ignition 281:
not more than 0.1%.
281:
not more than 0.1%.
Heavy metals, Method II 231:
0.002%.
231:
0.002%.
Assay—
Dissolve about 0.75 g of Procarbazine Hydrochloride, accurately weighed, in 100 mL of water. Titrate with 0.1 N sodium hydroxide VS, determining the endpoint potentiometrically, using a calomel-glass electrode system. Each mL of 0.1 N sodium hydroxide is equivalent to 25.78 mg of C12H19N3O·HCl.
Auxiliary Information—
Please check for your question in the FAQs before contacting USP.
Chromatographic Column—
| Topic/Question | Contact | Expert Committee |
| Monograph | Feiwen Mao, M.S.
Scientist 1-301-816-8320 |
(MDOOD05) Monograph Development-Ophthalmics Oncologics and Dermatologicals |
| Reference Standards | Lili Wang, Technical Services Scientist 1-301-816-8129 RSTech@usp.org |
USP32–NF27 Page 3394
Chromatographic columns text is not derived from, and not part of, USP 32 or NF 27.