Title: Collagenase
CAS Registry Number: 9001-12-1
Additional Names: Clostridiopeptidase A
Trademarks: Iruxol (Knoll); Santyl (Knoll)
Literature References: Rare proteolytic enzyme capable of digesting native undenatured collagen, q.v., found in certain Clostridia bacteria culture filtrates. Crude prepn from C. histolyticum: Mandl et al., J. Clin. Invest. 32, 1323 (1953). Purified prepn: Keller, Mandl, Arch. Biochem. Biophys. 101, 81 (1963). Also isolated from culture media of human wound tissue, skin, bone, leukocytes, gingiva, cornea, rheumatoid synovium; from involuting rat uterus and tadpole tailfin tissue. Sepn of fractions A and B having the respective mol wts 105,000 and 57,400: Harper et al., Biochem. Biophys. Res. Commun. 18, 627 (1965). Amino acid composition: Mandl et al., Biochemistry 3, 1737 (1964); Yoshida, Noda, Biochim. Biophys. Acta 105, 562 (1965). Potential use in treatment of herniated discs: Sussman, FR 2008611 (1970 to Worthington Biochemical). Review of early studies: Mandl, Adv. Enzymol. 23, 163-264 (1961). Reviews: idem, Science 169, 1234-1238 (1970); Nordwig, Adv. Enzymol. Relat. Areas Mol. Biol. 34, 155-205 (1971); Lazarus, Br. J. Dermatol. 86, 193-199 (1972). Book: Collagenase, I. Mandl, Ed. (Gordon and Breach, N.Y., 1972) 215 pp.
Use: In investigation of the structure and biosynthesis of collagen; in dispersion of cells for tissue culture studies.
Therap-Cat: Debriding agent.
Keywords: Debriding Agent; Enzyme; Proteolytic. |