Title: FM1-43
CAS Registry Number: 149838-22-2
CAS Name: 4-[2-[4-(Dibutylamino)phenyl]ethenyl]-1-[3-(triethylammonio)propyl]pyridinium dibromide
Additional Names: Neurodye GH1-43
Trademarks: SynaptoGreen (Biotium)
Molecular Formula: C30H49Br2N3
Molecular Weight: 611.54
Percent Composition: C 58.92%, H 8.08%, Br 26.13%, N 6.87%
Literature References: Green fluorescent styryl dye that binds to but is not translocated through biological membranes. Prepn and optical activity of p-aminostyrylpyridium probes: L. M. Loew et al., Biochemistry 17, 4065 (1978); A. Grinvald et al., Biophys. J. 39, 301 (1982). Optical analysis of synaptic vesicle recycling: W. J. Betz, G. S. Bewick, Science 255, 200 (1992); W. J. Betz et al., J. Neurosci. 12, 363 (1992); eidem, Curr. Opin. Neurobiol. 6, 365 (1996). Interaction with lipid membranes: U. Schote, J. Seelig, Biochim. Biophys. Acta 1415, 135 (1998); with background quenching: A. R. Kay et al., Neuron 24, 809 (1999); J. L. Pyle et al., ibid. 803. Use in membrane recycling study: T. J. Diefenbach et al., J. Neurosci. 19, 9436 (1999). Review of use and mechanism of action: M. A. Cousin, P. J. Robinson, J. Neurochem. 73, 2227-2239 (1999).
Properties: Red-orange solid. Amphiphilic. Sol in water, DMSO. Practically nonfluorescent in aqueous solutions, becoming fluorescent upon membrane binding. Abs max (methanol): 510 nm (e 56000). Emission max: 626 nm.
Absorption maximum: Abs max (methanol): 510 nm (e 56000)
Use: Biological probe for membrane dynamics. |