Title: Saporins
Literature References: Single chain ribosome inactivating proteins (type 1 RIPs); found in the seeds, leaves and roots of the soapwort plant, Saponaria officinalis L., Caryophyllaceae. Used to produce targeted immunotoxins by conjugating with a specific monoclonal antibody or other cell receptor ligand. At least 7 isoforms have been identified. Saporin-6 is the most abundant and is often referred to as "saporin". Isoln from seeds: F. Stirpe et al., Biochem. J. 216, 617 (1983). Purification and distribution in plant tissues: J. M. Ferreras et al., Biochim. Biophys. Acta 1216, 31 (1993). Production by plant cell culture: A. Di Cola et al., Plant Cell Rep. 17, 55 (1997). Review of use as neural lesioning tools: R. G. Wiley, Trends Neurosci. 15, 285-290 (1992); in model for Alzheimer's disease: R. Schliebs et al., Prog. Brain Res. 109, 253-264 (1996). Discussion of therapeutic potential in cancer: D. J. Flavell, Curr. Top. Microbiol. Immunol. 234, 57-61 (1998).
Derivative Type: Saporin-6
CAS Registry Number: 166944-16-7
Additional Names: SO6
Literature References: Isoform constituting 7% of the total seed protein. Composed of 259 amino acid residues; mol wt 29 kDa. Review of isoln, properties and production by recombinant technology: M. R. Soria et al., Targeted Diagn. Ther. 7, 193-212 (1992). Crystal structure: C. Savino et al., Acta Crystallogr. D54, 636 (1998).
Properties: pI >9.5. LD50 i.p. in mice: 4 mg/kg (Stirpe).
Toxicity data: LD50 i.p. in mice: 4 mg/kg (Stirpe)
Use: Biological research tool. |