Title: Azure B
CAS Registry Number: 531-55-5
CAS Name: 3-(Dimethylamino)-7-(methylamino)phenothiazin-5-ium chloride
Additional Names: 7-(dimethylamino)-3-(methylimino)-3H-phenothiazine hydrochloride; 3-methylamino-7-dimethylaminophenazathonium chloride; trimethyldiaminophenazathonium chloride; trimethylthionine chloride; methylene azure B; C.I. 52010
Molecular Formula: C15H16ClN3S
Molecular Weight: 305.83
Percent Composition: C 58.91%, H 5.27%, Cl 11.59%, N 13.74%, S 10.48%
Literature References: One of the main constituents of methylene azure. Prepn: Bernthsen, Ann. 230, 169 (1885); Kehrmann, Ber. 39, 1804 (1906); Kehrmann, Duttenhöfer, ibid. 925, 1403; Kehrmann et al., ibid. 46, 2137 (1913); MacNeal, Killian, J. Am. Chem. Soc. 48, 740 (1926). Review: H. J. Conn's Biological Stains, R. D. Lillie, Ed. (Williams & Wilkins, Baltimore, 9th ed., 1977) pp 421-423, 603-604. See also: Colour Index vol. 4 (3rd ed., 1971) p 4470.
Properties: Green glistening crystals or dark green powder. Sol in water (blue soln); sparingly sol in alc. Absorption max: 648-655 nm (3 ml of a soln of 50 mg in 250 ml H2O dil to 200 ml; read in a Beckman spectrophotometer 1 cm cell).
Absorption maximum: Absorption max: 648-655 nm (3 ml of a soln of 50 mg in 250 ml H2O dil to 200 ml; read in a Beckman spectrophotometer 1 cm cell)
Use: Biological stain. |