Title: Cy 5
CAS Registry Number: 146368-15-2
CAS Name: 1-[6-[-(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-2-[5-[1-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadienyl]-3,3-dimethyl-5-sulfo-3H-indolium inner salt
Molecular Formula: C45H52N4O14S2
Molecular Weight: 937.04
Percent Composition: C 57.68%, H 5.59%, N 5.98%, O 23.90%, S 6.84%
Literature References: Indocyanine dye with high fluorescence which is easily coupled to proteins. Named CyX.Y where X is the number of bridge carbons and Y represents a unique dye structure. Prepn: A. S. Waggoner et al., DE 3912046; eidem, US 5268486 (1990, 1993 both to Carnegie-Mellon). Use with fiber optic biosensors: L. A. Tempelman et al., Proc. SPIE Int. Soc. Opt. Eng. 2293, 139 (1994); for antibody/antigen detection: R. M. Wadkins et al., ibid. 2676, 148 (1996). Use in resonance energy transfer: P. R. Selvin et al, J. Am. Chem. Soc. 116, 6029 (1994).
Properties: Water soluble.
Derivative Type: Potassium salt
Properties: Water soluble. Absorbance max 650 nm; emission max 667 nm (e >200,000). Quantum yield 0.28.
Derivative Type: Cy 5.5
CAS Registry Number: 172777-84-3
Properties: Water soluble. Absorbance max 678 nm; emission max 703 nm (e 250,000). Quantum yield 0.28.
Use: Fluorescent label. |