Title: PMSF
CAS Registry Number: 329-98-6
CAS Name: Benzenemethanesulfonyl fluoride
Additional Names: phenylmethanesulfonyl fluoride; a-toluenesulfonyl fluoride
Molecular Formula: C7H7FO2S
Molecular Weight: 174.19
Percent Composition: C 48.27%, H 4.05%, F 10.91%, O 18.37%, S 18.41%
Literature References: Serine and cysteine protease inhibitor. Prepn: W. Davies, J. H. Dick, J. Chem. Soc. 1932, 483; and protease inhibition study: D. E. Fahrney, A. M. Gold, J. Am. Chem. Soc. 85, 997 (1963). Stability studies: G. T. James, Anal. Biochem. 86, 574 (1978). Analgesic effects and toxicity in rodents: C. Pinsky et al., Life Sci. 31, 1193 (1982). Neuropathologic effects in organophosphorus ester-induced delayed neuropathy in hens: C. Massicotte et al., Neurotoxicology 20, 749 (1999).
Properties: Colorless needles from light petroleum, mp 90-91°. LD50 (24 hr) in mice (mg/kg): 215 ±55 i.p. (Pinsky).
Melting point: mp 90-91°
Toxicity data: LD50 (24 hr) in mice (mg/kg): 215 ±55 i.p. (Pinsky)
Use: Standard protease inhibitor in biological research; in protein purification to prevent proteolytic degradation. |