Title: Rhod-2
CAS Registry Number: 132523-91-2
CAS Name: 9-[4-[Bis(carboxymethyl)amino]-3-[2-[2-[bis(carboxymethyl)amino]-5-methylphenoxy]ethoxy]phenyl]-3,6-bis(dimethylamino)xanthylium chloride
Molecular Formula: C40H43ClN4O11
Molecular Weight: 791.24
Percent Composition: C 60.72%, H 5.48%, Cl 4.48%, N 7.08%, O 22.24%
Literature References: Rhodamine-based calcium specific fluorescent dye. Prepn: R. Y. Tsien, A. Minta, EP 314480; eidem, US 5049673 (1989, 1991 both to Univ. California); A. Minta et al, J. Biol. Chem. 264, 8171 (1989). Use in monitoring intramitochondrial Ca2+: M. Hoth, R. S. Lewis in Imaging Neurons, R. Yuste et al., Eds. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 2000) pp 1-14; in free/intracellular Ca2+ levels: M.-P. Muriel et al., J. Comp. Neurol. 426, 297 (2000); G. A. MacGowan et al., J. Biomed. Opt. 6, 23 (2001).
Properties: Dark purplish solid. KD for Ca2+: 1mM.
Use: Indicator for calcium measurement in biological systems. |