Esterification of the primary hydroxyl group of dexamethasone (I) by treatment with 4-nitrophenyl chloroformate (II) in the presence of N-methylmorpholine afforded the nitrophenyl carbonate (III). Condensation of the active ester (III) with mono-N-Boc-1,6-diaminohexane (IV) produced carbamate (V). After acidic deprotection of the Boc group, the resultant amine (VI) was acylated with succinimidyl 4-maleimidobutyrate (VII), yielding the title amide.