The acylation of phorbol (I) with tetradecanoyl chloride (II) in pyridine gives the triacyl phorbol (III), which is selective hydrolyzed with NaOMe in methanol at room temperature to yield the monoacyl phorbol (IV). The acylation of (IV) with acetic anhydride in pyridine at 8 C affords the diacetoxy tetradecanoyloxy phorbol (V). Finally, this compound is monodeacetylated by means of HClO4 in methanol at room temperature to provide the target mixed diacylphorbol.