*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : BY3675000
CHEMICAL NAME : Aniline, 4,4'-(imidocarbonyl)bis(N,N-dimethyl-,
hydrochloride
CAS REGISTRY NUMBER : 2465-27-2
LAST UPDATED : 199710
DATA ITEMS CITED : 32
MOLECULAR FORMULA : C17-H21-N3.Cl-H
MOLECULAR WEIGHT : 303.87
WISWESSER LINE NOTATION : 1N1&R DYUM&R DN1&1 &GH
COMPOUND DESCRIPTOR : Tumorigen
Mutagen
SYNONYMS/TRADE NAMES :
* Adc Auramine O
* Aizen auramine
* Aizen Auramine Conc. SFA
* Aizen auramine OH
* Auramine A1
* Auramine conc. specially soluble in Spirit
* Auramine Extra
* Auramine Extra Conc. A
* Auramine FA
* Auramine hydrochloride
* Auramine Lake Yellow O
* Auramine N
* Auramine O
* Auramine O (biological stain)
* Auramine O Extra Conc. A Export
* Auramine ON
* Auramine OO
* Auramine OOO
* Auramine OS
* Auramine Pure
* Auramine SP
* Auramine Yellow
* Benzenamine, 4,4'-carbonimidoylbis(N,N-dimethyl-, monohydrochloride
* 4,4'-Bis(dimethylamino)-benzhydrylidenimine hydrochloride
* 4:4'-Bis(dimethylamino)benzophenone-imine hydrochloride
* 1,1-Bis(p-dimethylaminophenyl)methylenimine hydrochloride
* Calcozine Yellow OX
* C.I. 41000
* C.I. Basic Yellow 2
* C.I. Basic Yellow 2, monohydrochloride
* 4,4'-(Imidocarbonyl)bis(N,N-dimethylamine), monohydrochloride
* Mitsui auramine O
* Zlut zasadita 2
*** HEALTH HAZARD DATA ***
** ACUTE TOXICITY DATA **
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 1500 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
26,619,1966
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 135 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
NALSDJ NATO ASI Series, Series A: Life Sciences. (Plenum Pub. Corp., 233
Spring St., New York, NY 10013) V.53- 1983- Volume(issue)/page/year:
60,745,1983
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 480 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
CRDLP* U.S. Army Chemical Research & Development Laboratory, Special
Publication. (Edgewood Arsenal, MD 21010) Volume(issue)/page/year:
4-59,1964
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Administration onto the skin
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 300 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
CRDLP* U.S. Army Chemical Research & Development Laboratory, Special
Publication. (Edgewood Arsenal, MD 21010) Volume(issue)/page/year:
4-59,1964
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 300 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
OKEHDW Osaka-furitsu Koshu Eisei Kenkyusho Kenkyu Hokoku, Koshu Eisei Hen.
Research Reports of the Oaka Prefectural Institute of Public Health, Public
Health Section. (Osaka-furitsu Koshu Eisei Kenkyusho, 1-3-69 Nakamichi,
Higashinari-ku, Osaka, 537, Japan) No.3- 1966- Volume(issue)/page/year:
(3),185,1953
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Mammal - cat
DOSE/DURATION : 150 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
CWLTM* Chemical Warfare Laboratories Technical Memorandum. (U.S. Army
Chemical Center, Edgewood Arsenal, MD 21010) Volume(issue)/page/year:
#47-6,1959
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Mammal - domestic
DOSE/DURATION : 150 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
CWLTM* Chemical Warfare Laboratories Technical Memorandum. (U.S. Army
Chemical Center, Edgewood Arsenal, MD 21010) Volume(issue)/page/year:
#47-6,1959
** TUMORIGENIC DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 40 gm/kg/87W-C
TOXIC EFFECTS :
Tumorigenic - neoplastic by RTECS criteria
Liver - tumors
Kidney, Ureter, Bladder - tumors
REFERENCE :
BJCAAI British Journal of Cancer. (Macmillan Press Ltd., Houndmills,
Basingstoke, Hants. RG21 2XS, UK) V.1- 1947- Volume(issue)/page/year:
16,87,1962
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 440 mg/kg/21W-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Liver - tumors
Tumorigenic - tumors at site of application
REFERENCE :
BJCAAI British Journal of Cancer. (Macmillan Press Ltd., Houndmills,
Basingstoke, Hants. RG21 2XS, UK) V.1- 1947- Volume(issue)/page/year:
16,87,1962
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 73 gm/kg/52W-C
TOXIC EFFECTS :
Tumorigenic - neoplastic by RTECS criteria
Liver - tumors
REFERENCE :
BJCAAI British Journal of Cancer. (Macmillan Press Ltd., Houndmills,
Basingstoke, Hants. RG21 2XS, UK) V.1- 1947- Volume(issue)/page/year:
16,87,1962
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 32760 mg/kg/39W-C
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Liver - tumors
REFERENCE :
EPASR* United States Environmental Protection Agency, Office of Pesticides
and Toxic Substances. (U.S. Environmental Protection Agency, 401 M St., SW,
Washington, DC 20460) History unknown. Volume(issue)/page/year:
8EHQ-0588-0730
** MUTATION DATA **
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 2 mg/plate
REFERENCE :
CRNGDP Carcinogenesis (London). (Oxford Univ. Press, Pinkhill House,
Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1980-
Volume(issue)/page/year: 2,1317,1981
TYPE OF TEST : DNA repair
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 250 ug/disc
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 41,61,1976
TYPE OF TEST : DNA adduct
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 30 ppm
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 89,95,1981
TYPE OF TEST : DNA damage
TEST SYSTEM : Human Fibroblast
DOSE/DURATION : 300 umol/L
REFERENCE :
JTEHD6 Journal of Toxicology and Environmental Health. (Hemisphere Pub.,
1025 Vermont Ave., NW, Washington, DC 20005) V.1- 1975/76-
Volume(issue)/page/year: 9,941,1982
TYPE OF TEST : DNA inhibition
TEST SYSTEM : Human HeLa cell
DOSE/DURATION : 62 umol/L
REFERENCE :
CRNGDP Carcinogenesis (London). (Oxford Univ. Press, Pinkhill House,
Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1980-
Volume(issue)/page/year: 13,2389,1992
TYPE OF TEST : Morphological transformation
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Rodent - rat
DOSE/DURATION : 4914 mg/kg
REFERENCE :
EPASR* United States Environmental Protection Agency, Office of Pesticides
and Toxic Substances. (U.S. Environmental Protection Agency, 401 M St., SW,
Washington, DC 20460) History unknown. Volume(issue)/page/year:
8EHQ-0588-0730
TYPE OF TEST : DNA damage
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - rat
DOSE/DURATION : 9 mg/kg
REFERENCE :
CRNGDP Carcinogenesis (London). (Oxford Univ. Press, Pinkhill House,
Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1980-
Volume(issue)/page/year: 6,1285,1985
TYPE OF TEST : DNA damage
TEST SYSTEM : Rodent - rat Liver
DOSE/DURATION : 3 umol/L
REFERENCE :
SinJF# Personal Communication from J.F. Sina, Merck Institute for
Therapeutic Research, West Point, PA 19486, Oct. 26, 1982
Volume(issue)/page/year: 26OCT1982
TYPE OF TEST : DNA damage
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 15 mg/kg
REFERENCE :
JTEHD6 Journal of Toxicology and Environmental Health. (Hemisphere Pub.,
1025 Vermont Ave., NW, Washington, DC 20005) V.1- 1975/76-
Volume(issue)/page/year: 9,941,1982
TYPE OF TEST : Sister chromatid exchange
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 7500 ug/kg
REFERENCE :
JTEHD6 Journal of Toxicology and Environmental Health. (Hemisphere Pub.,
1025 Vermont Ave., NW, Washington, DC 20005) V.1- 1975/76-
Volume(issue)/page/year: 9,941,1982
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Ovary
DOSE/DURATION : 20 umol/L/5H (Continuous)
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 1,27,1979
*** OCCUPATIONAL EXPOSURE LIMITS ***
OEL-FRANCE;Carcinogen JAN 1993
OEL-GERMANY;Carcinogen JAN 1993
*** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA ***
NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA :
NOHS - National Occupational Hazard Survey (1974)
NOHS Hazard Code - M1444
No. of Facilities: 1184 (estimated)
No. of Industries: 17
No. of Occupations: 22
No. of Employees: 10114 (estimated)
NOES - National Occupational Exposure Survey (1983)
NOES Hazard Code - M1444
No. of Facilities: 1191 (estimated)
No. of Industries: 27
No. of Occupations: 24
No. of Employees: 19092 (estimated)
No. of Female Employees: 4705 (estimated)
*** STATUS IN U.S. ***
EPA GENETOX PROGRAM 1988, Positive: E coli polA without S9; S
cerevisiae-homozygosis
EPA GENETOX PROGRAM 1988, Positive/limited: Carcinogenicity-mouse/rat
EPA GENETOX PROGRAM 1988, Negative: Histidine reversion-Ames test
EPA GENETOX PROGRAM 1988, Inconclusive: SHE-clonal assay; Mammalian
micronucleus
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JUNE 1998
*** END OF RECORD ***