*** CHEMICAL IDENTIFICATION *** RTECS NUMBER : UR2450000 CHEMICAL NAME : Pyrene CAS REGISTRY NUMBER : 129-00-0 LAST UPDATED : 199710 DATA ITEMS CITED : 52 MOLECULAR FORMULA : C16-H10 MOLECULAR WEIGHT : 202.26 WISWESSER LINE NOTATION : L666 B6 2AB PJ COMPOUND DESCRIPTOR : Tumorigen Mutagen Primary Irritant SYNONYMS/TRADE NAMES : * Benzo(def)phenanthrene * Coal tar pitch volatiles:pyrene * Pyren * beta-Pyrene *** HEALTH HAZARD DATA *** ** SKIN/EYE IRRITATION DATA ** TYPE OF TEST : Standard Draize test ROUTE OF EXPOSURE : Administration onto the skin SPECIES OBSERVED : Rodent - rabbit DOSE/DURATION : 500 mg/24H REACTION SEVERITY : Mild REFERENCE : 28ZPAK "Sbornik Vysledku Toxixologickeho Vysetreni Latek A Pripravku," Marhold, J.V., Institut Pro Vychovu Vedoucicn Pracovniku Chemickeho Prumyclu Praha, Czechoslovakia, 1972 Volume(issue)/page/year: -,26,1972 ** ACUTE TOXICITY DATA ** TYPE OF TEST : LD50 - Lethal dose, 50 percent kill ROUTE OF EXPOSURE : Oral SPECIES OBSERVED : Rodent - rat DOSE/DURATION : 2700 mg/kg TOXIC EFFECTS : Sense Organs and Special Senses (Eye) - conjunctive irritation Behavioral - excitement Behavioral - muscle contraction or spasticity REFERENCE : GTPZAB Gigiena Truda i Professional'nye Zabolevaniya. Labor Hygiene and Occupational Diseases. (V/O Mezhdunarodnaya Kniga, 113095 Moscow, USSR) V.1-36, 1957-1992. For publisher information, see MTPEEI Volume(issue)/page/year: 15(2),59,1971 TYPE OF TEST : LC50 - Lethal concentration, 50 percent kill ROUTE OF EXPOSURE : Inhalation SPECIES OBSERVED : Rodent - rat DOSE/DURATION : 170 mg/m3 TOXIC EFFECTS : Sense Organs and Special Senses (Eye) - conjunctive irritation Behavioral - excitement Behavioral - muscle contraction or spasticity REFERENCE : GTPZAB Gigiena Truda i Professional'nye Zabolevaniya. Labor Hygiene and Occupational Diseases. (V/O Mezhdunarodnaya Kniga, 113095 Moscow, USSR) V.1-36, 1957-1992. For publisher information, see MTPEEI Volume(issue)/page/year: 15(2),59,1971 TYPE OF TEST : LD50 - Lethal dose, 50 percent kill ROUTE OF EXPOSURE : Oral SPECIES OBSERVED : Rodent - mouse DOSE/DURATION : 800 mg/kg TOXIC EFFECTS : Sense Organs and Special Senses (Eye) - conjunctive irritation Behavioral - excitement Behavioral - muscle contraction or spasticity REFERENCE : GTPZAB Gigiena Truda i Professional'nye Zabolevaniya. Labor Hygiene and Occupational Diseases. (V/O Mezhdunarodnaya Kniga, 113095 Moscow, USSR) V.1-36, 1957-1992. For publisher information, see MTPEEI Volume(issue)/page/year: 15(2),59,1971 TYPE OF TEST : LD50 - Lethal dose, 50 percent kill ROUTE OF EXPOSURE : Intraperitoneal SPECIES OBSERVED : Rodent - mouse DOSE/DURATION : 514 mg/kg TOXIC EFFECTS : Details of toxic effects not reported other than lethal dose value REFERENCE : PMRSDJ Progress in Mutation Research. (Elsevier Science Pub. Co., Inc., 52 Vanderbilt Ave., New York, NY 10017) V.1- 1981- Volume(issue)/page/year: 1,682,1981 ** OTHER MULTIPLE DOSE TOXICITY DATA ** TYPE OF TEST : TDLo - Lowest published toxic dose ROUTE OF EXPOSURE : Oral SPECIES OBSERVED : Rodent - rat DOSE/DURATION : 12150 mg/kg/45D-I TOXIC EFFECTS : Behavioral - muscle contraction or spasticity Blood - normocytic anemia Blood - changes in erythrocyte (RBC) count REFERENCE : GTPZAB Gigiena Truda i Professional'nye Zabolevaniya. Labor Hygiene and Occupational Diseases. (V/O Mezhdunarodnaya Kniga, 113095 Moscow, USSR) V.1-36, 1957-1992. For publisher information, see MTPEEI Volume(issue)/page/year: 15(2),59,1971 ** TUMORIGENIC DATA ** TYPE OF TEST : TDLo - Lowest published toxic dose ROUTE OF EXPOSURE : Administration onto the skin SPECIES OBSERVED : Rodent - mouse DOSE/DURATION : 10 gm/kg/3W-I TOXIC EFFECTS : Tumorigenic - equivocal tumorigenic agent by RTECS criteria Skin and Appendages - tumors REFERENCE : BJCAAI British Journal of Cancer. (Macmillan Press Ltd., Houndmills, Basingstoke, Hants. RG21 2XS, UK) V.1- 1947- Volume(issue)/page/year: 10,363,1956 ** MUTATION DATA ** TYPE OF TEST : Mutation in microorganisms TEST SYSTEM : Bacteria - Salmonella typhimurium DOSE/DURATION : 5 ug/plate REFERENCE : DTESD7 52 Vanderbilt Ave., New York, NY 10017) V.1-15, 1977-87. Discontinued 52 Vanderbilt Ave., New York, NY 10017) V.1- 1977- Volume(issue)/page/year: 2,249,1977 TYPE OF TEST : Mutation in microorganisms TEST SYSTEM : Bacteria - Salmonella typhimurium DOSE/DURATION : 300 ng/plate REFERENCE : ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For publisher information, see EMMUEG. Volume(issue)/page/year: 6(Suppl 2),1,1984 TYPE OF TEST : Mutation test systems - not otherwise specified TEST SYSTEM : Bacteria - Escherichia coli DOSE/DURATION : 50 nmol/tube REFERENCE : MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 147,79,1985 TYPE OF TEST : Mutation in microorganisms TEST SYSTEM : Bacteria - Escherichia coli DOSE/DURATION : 1 ug/plate REFERENCE : PMRSDJ Progress in Mutation Research. (Elsevier Science Pub. Co., Inc., 52 Vanderbilt Ave., New York, NY 10017) V.1- 1981- Volume(issue)/page/year: 1,387,1981 TYPE OF TEST : DNA adduct TEST SYSTEM : Bacteria - Escherichia coli DOSE/DURATION : 10 umol/L REFERENCE : PNCCA2 Proceedings, National Cancer Conference. (Philadelphia, PA) V.1-7, 1949-72. For publisher information, see CANCAR. Volume(issue)/page/year: 5,39,1965 TYPE OF TEST : Micronucleus test ROUTE OF EXPOSURE : Multiple routes TEST SYSTEM : Non-mammalian species DOSE/DURATION : 900 nmol/L REFERENCE : MUTAEX Mutagenesis. (Oxford Univ. Press, Pinkhill House, Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1986- Volume(issue)/page/year: 4,17,1989 TYPE OF TEST : DNA adduct TEST SYSTEM : Non-mammalian species DOSE/DURATION : 8600 nmol/L REFERENCE : GANMAX Gann Monograph. (Tokyo, Japan) No.1-10, 1966-71. For publisher information, see GMCRDC. Volume(issue)/page/year: 6,49,1968 TYPE OF TEST : DNA adduct TEST SYSTEM : Fish - salmon Testis DOSE/DURATION : 5 ug/1H (Continuous) REFERENCE : BIJOAK Biochemical Journal. (Biochemical Soc. Book Depot, POB 32, Commerce Way, Colchester, Essex CO2 8HP, UK) V.1- 1906- Volume(issue)/page/year: 110,159,1968 TYPE OF TEST : Unscheduled DNA synthesis TEST SYSTEM : Human Fibroblast DOSE/DURATION : 100 mg/L REFERENCE : TXCYAC Toxicology. (Elsevier Scientific Pub. Ireland, Ltd., POB 85, Limerick, Ireland) V.1- 1973- Volume(issue)/page/year: 21,151,1981 TYPE OF TEST : Sister chromatid exchange TEST SYSTEM : Human Lymphocyte DOSE/DURATION : 100 umol/L REFERENCE : MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 211,77,1989 TYPE OF TEST : Mutation in mammalian somatic cells TEST SYSTEM : Human Cells - not otherwise specified DOSE/DURATION : 12 umol/L REFERENCE : MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 130,127,1984 TYPE OF TEST : Unscheduled DNA synthesis TEST SYSTEM : Rodent - rat Liver DOSE/DURATION : 2500 umol/L REFERENCE : CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year: 42,3010,1982 TYPE OF TEST : Mutation in mammalian somatic cells TEST SYSTEM : Rodent - rat Embryo DOSE/DURATION : 10 mg/L REFERENCE : JTEHD6 Journal of Toxicology and Environmental Health. (Hemisphere Pub., 1025 Vermont Ave., NW, Washington, DC 20005) V.1- 1975/76- Volume(issue)/page/year: 4,79,1978 TYPE OF TEST : Mutation in microorganisms TEST SYSTEM : Rodent - mouse Lymphocyte DOSE/DURATION : 10100 nmol/L REFERENCE : MUTAEX Mutagenesis. (Oxford Univ. Press, Pinkhill House, Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1986- Volume(issue)/page/year: 3,193,1988 TYPE OF TEST : Morphological transformation TEST SYSTEM : Rodent - mouse Embryo DOSE/DURATION : 10 mg/L REFERENCE : EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.10- 1987- Volume(issue)/page/year: 12,21,1988 TYPE OF TEST : DNA damage TEST SYSTEM : Rodent - mouse Lymphocyte DOSE/DURATION : 50 umol/L REFERENCE : MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 203,155,1988 TYPE OF TEST : Mutation in mammalian somatic cells TEST SYSTEM : Rodent - mouse Lymphocyte DOSE/DURATION : 1510 umol/L REFERENCE : MUTAEX Mutagenesis. (Oxford Univ. Press, Pinkhill House, Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1986- Volume(issue)/page/year: 3,193,1988 TYPE OF TEST : Morphological transformation TEST SYSTEM : Rodent - hamster Embryo DOSE/DURATION : 10 mg/L REFERENCE : CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year: 31,1118,1971 TYPE OF TEST : Morphological transformation TEST SYSTEM : Rodent - hamster Kidney DOSE/DURATION : 62500 ug/L REFERENCE : PMRSDJ Progress in Mutation Research. (Elsevier Science Pub. Co., Inc., 52 Vanderbilt Ave., New York, NY 10017) V.1- 1981- Volume(issue)/page/year: 1,626,1981 TYPE OF TEST : Unscheduled DNA synthesis TEST SYSTEM : Rodent - hamster Liver DOSE/DURATION : 100 umol/L REFERENCE : ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For publisher information, see EMMUEG. Volume(issue)/page/year: 5,1,1983 TYPE OF TEST : Cytogenetic analysis TEST SYSTEM : Rodent - hamster Lung DOSE/DURATION : 10 mg/L REFERENCE : ESKHA5 Eisei Shikenjo Hokoku. Bulletin of the Institute of Hygienic Sciences. (Kokuritsu Eisei Shikenjo Kagaku, 18-1 Bushitsu Johobu, Setagaya-ku, Tokyo 158, Japan) V.1- 1886- Volume(issue)/page/year: (103),64,1985 *** REVIEWS *** IARC Cancer Review:Animal Inadequate Evidence IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210) V.1- 1972- Volume(issue)/page/year: 32,431,1983 IARC Cancer Review:Human No Adequate Data IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210) V.1- 1972- Volume(issue)/page/year: 32,431,1983 IARC Cancer Review:Group 3 IMSUDL IARC Monographs, Supplement. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210) No.1- 1979- Volume(issue)/page/year: 7,56,1987 *** U.S. STANDARDS AND REGULATIONS *** OSHA PEL (Gen Indu):8H TWA 0.2 mg/m3 CFRGBR Code of Federal Regulations. (U.S. Government Printing Office, Supt. of Documents, Washington, DC 20402) Volume(issue)/page/year: 29,1910.1000,1994 OSHA PEL (Construc):8H TWA 0.2 mg/m3 CFRGBR Code of Federal Regulations. (U.S. Government Printing Office, Supt. of Documents, Washington, DC 20402) Volume(issue)/page/year: 29,1926.55,1994 OSHA PEL (Shipyard):8H TWA 0.2 mg/m3 CFRGBR Code of Federal Regulations. (U.S. Government Printing Office, Supt. of Documents, Washington, DC 20402) Volume(issue)/page/year: 29,1915.1000,1993 OSHA PEL (Fed Cont):8H TWA 0.2 mg/m3 CFRGBR Code of Federal Regulations. (U.S. Government Printing Office, Supt. of Documents, Washington, DC 20402) Volume(issue)/page/year: 41,50-204.50,1994 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - T1328 No. of Facilities: 11 (estimated) No. of Industries: 1 No. of Occupations: 1 No. of Employees: 32 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - T1328 No. of Facilities: 560 (estimated) No. of Industries: 3 No. of Occupations: 3 No. of Employees: 9387 (estimated) *** STATUS IN U.S. *** EPA GENETOX PROGRAM 1988, Negative: Cell transform.-BALB/c-3T3; SHE-clonal assay EPA GENETOX PROGRAM 1988, Negative: Cell transform.-mouse embryo EPA GENETOX PROGRAM 1988, Negative: Cell transform.-RLV F344 rat embryo EPA GENETOX PROGRAM 1988, Negative: In vitro cytogenetics-nonhuman; Host-mediated assay EPA GENETOX PROGRAM 1988, Negative: Histidine reversion-Ames test; In vivo SCE-nonhuman EPA GENETOX PROGRAM 1988, Negative: Sperm morphology-mouse; In vitro UDS-human fibroblast EPA GENETOX PROGRAM 1988, Negative: V79 cell culture-gene mutation EPA GENETOX PROGRAM 1988, Inconclusive: Carcinogenicity-mouse/rat; Mammalian micronucleus EPA GENETOX PROGRAM 1988, Inconclusive: In vitro SCE-nonhuman EPA TSCA Section 8(b) CHEMICAL INVENTORY EPA TSCA Section 8(d) unpublished health/safety studies On EPA IRIS database EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JUNE 1998 NIOSH Analytical Method, 1994: Polynuclear aromatic hydrocarbons by HPLC, 5506; by GC, 5515 OSHA ANALYTICAL METHOD #ID-58 *** END OF RECORD ***