*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : WG9800000
CHEMICAL NAME : Spiro(benzofuran-2(3H),1'-(2)cyclohexene)-3,4'-dione,
7-chloro-2'4,6-trimethoxy-6'-beta- methyl-
CAS REGISTRY NUMBER : 126-07-8
BEILSTEIN REFERENCE NO. : 0095226
REFERENCE : 5-18-05-00150 (Beilstein Handbook Reference)
LAST UPDATED : 199801
DATA ITEMS CITED : 53
MOLECULAR FORMULA : C17-H17-Cl-O6
MOLECULAR WEIGHT : 352.79
WISWESSER LINE NOTATION : T56 BOXVJ FO1 HO1 IG C-& DL6V DX BUTJ CO1 E1
COMPOUND DESCRIPTOR : Agricultural Chemical
Tumorigen
Drug
Mutagen
Reproductive Effector
Natural Product
SYNONYMS/TRADE NAMES :
* Amudane
* Biogrisin-FP
* 7-Chloro-4,6,2'-trimethoxy-6'-methylgris-2'-en-3,4'-dione
* Curling factor
* Delmofulvina
* Fulcin
* Fulcine
* Fulvican grisactin
* Fulvicin
* Fulvicin-P/G
* Fulvicin-U/F
* Fulvina
* Fulvistatin
* Fungivin
* Greosin
* Gresfeed
* Gricin
* Grifulvin
* Grifulvin V
* Grisactin
* Griscofulvin
* Grisefuline
* Griseo
* Griseofulvin
* (+)-Griseofulvin
* Griseofulvin-forte
* Griseofulvinum
* Griseomix
* Grisetin
* Grisofulvin
* Grisovin
* Gris-PEG
* Grysio
* Guservin
* Lamoryl
* Likuden
* Murfulvin
* Neo-fulcin
* NSC 34533
* Poncyl
* Spirofulvin
* Sporostatin
* USAF SC-2
*** HEALTH HAZARD DATA ***
** ACUTE TOXICITY DATA **
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : >10 gm/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
NIIRDN Drugs in Japan (Ethical Drugs). (Yakugyo Jiho Co., Ltd., Tokyo,
Japan) Volume(issue)/page/year: 6,215,1982
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : >5 gm/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
2,44,1960
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 400 mg/kg
TOXIC EFFECTS :
Blood - changes in cell count (unspecified)
Blood - changes in bone marrow (not otherwise specified)
REFERENCE :
NATUAS Nature. (Nature Subscription Dept., POB 1018, Manasguan, NJ 08736)
V.1- 1869- Volume(issue)/page/year: 182,1320,1958
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : >50 gm/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
2,44,1960
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 200 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: AD277-689
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : >12 gm/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
2,44,1960
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 280 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
2,44,1960
** OTHER MULTIPLE DOSE TOXICITY DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - guinea pig
DOSE/DURATION : 109 gm/kg/52W-C
TOXIC EFFECTS :
Nutritional and Gross Metabolic - weight loss or decreased weight gain
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
2,44,1960
** TUMORIGENIC DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 462 gm/kg/2Y-I
TOXIC EFFECTS :
Tumorigenic - neoplastic by RTECS criteria
Blood - lymphoma, including Hodgkin's disease
REFERENCE :
BJCAAI British Journal of Cancer. (Macmillan Press Ltd., Houndmills,
Basingstoke, Hants. RG21 2XS, UK) V.1- 1947- Volume(issue)/page/year:
38,237,1978
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 440 gm/kg/52W-C
TOXIC EFFECTS :
Tumorigenic - neoplastic by RTECS criteria
Liver - tumors
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
26,721,1966
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 120 mg/kg/49W-I
TOXIC EFFECTS :
Tumorigenic - neoplastic by RTECS criteria
Liver - tumors
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
27,1900,1967
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 730 gm/kg/52W-C
TOXIC EFFECTS :
Tumorigenic - neoplastic by RTECS criteria
Liver - tumors
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
26,721,1966
** REPRODUCTIVE DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE : 1250 mg/kg
SEX/DURATION : female 6-15 day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death,
e.g., stunted fetus)
Reproductive - Effects on Newborn - viability index (e.g., # alive at day 4
per # born alive)
REFERENCE :
SCIEAS Science. (American Assoc. for the Advancement of Science, 1333 H
St., NW, Washington, DC 20005) V.1- 1895- Volume(issue)/page/year:
175,1483,1972
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE : 2 gm/kg
SEX/DURATION : female 11-14 day(s) after conception
TOXIC EFFECTS :
Reproductive - Specific Developmental Abnormalities - Central Nervous System
Reproductive - Specific Developmental Abnormalities - urogenital system
REFERENCE :
ANTBAL Antibiotiki. (Moscow, USSR) V.1-29, 1956-84. For publisher
information, see AMBIEH. Volume(issue)/page/year: 14,44,1969
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE : 12500 mg/kg
SEX/DURATION : female 6-15 day(s) after conception
TOXIC EFFECTS :
Reproductive - Fertility - post-implantation mortality (e.g. dead and/or
resorbed implants per total number of implants)
Reproductive - Specific Developmental Abnormalities - musculoskeletal system
REFERENCE :
SCIEAS Science. (American Assoc. for the Advancement of Science, 1333 H
St., NW, Washington, DC 20005) V.1- 1895- Volume(issue)/page/year:
175,1483,1972
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE : 500 mg/kg
SEX/DURATION : female 9 day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Embryo or Fetus - fetal death
Reproductive - Specific Developmental Abnormalities - eye/ear
REFERENCE :
ANTBAL Antibiotiki. (Moscow, USSR) V.1-29, 1956-84. For publisher
information, see AMBIEH. Volume(issue)/page/year: 14,44,1969
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE : 10 gm/kg
SEX/DURATION : female 8-9 day(s) after conception
TOXIC EFFECTS :
Reproductive - Specific Developmental Abnormalities - musculoskeletal system
REFERENCE :
40YJAX "Evaluation of Embryotoxicity, Mutagenicity and Carcinogenicity Risks
In New Drugs, Proceedings of the Symposium on Toxicological Testing for
Safety of New Drugs, 3rd, Prague, 1976," Benesova, O., et al., eds., Prague,
Czechoslovakia, Univerzita Karlova, 1979 Volume(issue)/page/year:
-,161,1979
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Mammal - domestic
DOSE : 1 gm/kg
SEX/DURATION : female 48-52 day(s) after conception
TOXIC EFFECTS :
Reproductive - Specific Developmental Abnormalities - musculoskeletal system
REFERENCE :
VEQUDU Veterinary Quarterly. (Kluwer Academic Publishers, POB 322, 3300 AH
Dordrecht, Netherlands) V.1- 1979-d/* Volume(issue)/page/year:
19,58,1997
** MUTATION DATA **
TYPE OF TEST : Mutation test systems - not otherwise specified
TEST SYSTEM : Microorganism - not otherwise specified
DOSE/DURATION : 10 mg/L
REFERENCE :
AMICCW Archives of Microbiology. (Springer-Verlag New York, Inc., Service
Center, 44 Hartz Way, Secacus, NJ 07094) V.95- 1974-
Volume(issue)/page/year: 95,57,1974
TYPE OF TEST : Specific locus test
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Insect - Drosophila melanogaster
DOSE/DURATION : 2 gm/L
REFERENCE :
GNKAA5 Genetika (Moscow). For English translation, see SOGEBZ. (V/O
Mezhdunarodnaya Kniga, 113095 Moscow, USSR) No.1- 1965-
Volume(issue)/page/year: 30(6),796,1994
TYPE OF TEST : DNA repair
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Insect - Drosophila melanogaster
DOSE/DURATION : 3 gm/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 343,229,1995
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Mold - Aspergillus nidulans
DOSE/DURATION : 17 umol/L
REFERENCE :
PHYTAJ Phytopathology. (American Phytopathological Soc., 3340 Pilot Knob
Rd., St. Paul, MN 55121) V.1- 1911- Volume(issue)/page/year: 66,217,1976
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Mold - Aspergillus nidulans
DOSE/DURATION : 400 mg/L
REFERENCE :
CJMIAZ Canadian Journal of Microbiology. (National Research Council of
Canada, Publication Sales and Distribution, Ottawa ON K1A OR6, Canada) V.1-
1954- Volume(issue)/page/year: 18,683,1972
TYPE OF TEST : Sex chromosome loss and nondisjunction
TEST SYSTEM : Mold - Aspergillus nidulans
DOSE/DURATION : 6 mg/L
REFERENCE :
JOBAAY Journal of Bacteriology. (American Soc. for Microbiology, 1913 I
St., NW, Washington, DC 20006) V.1- 1916- Volume(issue)/page/year:
119,334,1974
TYPE OF TEST : Cytogenetic analysis
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Insect - grasshopper
DOSE/DURATION : 5 mg
REFERENCE :
PISCAD Proceedings of the Indian Science Congress. (Indian Science Congress
Assoc., 14, Dr. Biresh Guha St., Calcutta, 700017, India) 1st- 1914-
Volume(issue)/page/year: 59(Pt 3),410,1972
TYPE OF TEST : Micronucleus test
TEST SYSTEM : Human Lymphocyte
DOSE/DURATION : 5 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 322,151,1994
TYPE OF TEST : DNA inhibition
TEST SYSTEM : Human Fibroblast
DOSE/DURATION : 20 mg/L/3D (Continuous)
REFERENCE :
KAMJDW Kawasaki Medical Journal. (Kawasaki Medical School, Kurashiki
701-01, Japan) V.1- 1975- Volume(issue)/page/year: 2,127,1976
TYPE OF TEST : DNA inhibition
TEST SYSTEM : Human Lymphocyte
DOSE/DURATION : 20 mg/L/3D (Continuous)
REFERENCE :
KAMJDW Kawasaki Medical Journal. (Kawasaki Medical School, Kurashiki
701-01, Japan) V.1- 1975- Volume(issue)/page/year: 2,127,1976
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Human Lymphocyte
DOSE/DURATION : 40 mg/L/3D
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 25,123,1974
TYPE OF TEST : Sex chromosome loss and nondisjunction
TEST SYSTEM : Human Lymphocyte
DOSE/DURATION : 5 mg/L
REFERENCE :
MUTAEX Mutagenesis. (Oxford Univ. Press, Pinkhill House, Southfield Road,
Eynsham, Oxford OX8 1JJ, UK) V.1- 1986- Volume(issue)/page/year: 4,165,1989
TYPE OF TEST : Cytogenetic analysis
ROUTE OF EXPOSURE : Intravenous
TEST SYSTEM : Rodent - rat
DOSE/DURATION : 200 mg/kg
REFERENCE :
NATUAS Nature. (Nature Subscription Dept., POB 1018, Manasguan, NJ 08736)
V.1- 1869- Volume(issue)/page/year: 182,1320,1958
TYPE OF TEST : Micronucleus test
TEST SYSTEM : Rodent - mouse Cells - not otherwise specified
DOSE/DURATION : 10 mg/L
REFERENCE :
JNCIAM Journal of the National Cancer Institute. (Washington, DC) V.1-60,
1940-78. For publisher information, see JJIND8. Volume(issue)/page/year:
56,357,1976
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - mouse Embryo
DOSE/DURATION : 50 mg/L
REFERENCE :
NATUAS Nature. (Nature Subscription Dept., POB 1018, Manasguan, NJ 08736)
V.1- 1869- Volume(issue)/page/year: 253,361,1975
TYPE OF TEST : Sister chromatid exchange
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 100 mg/kg
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 137,111,1984
TYPE OF TEST : Sex chromosome loss and nondisjunction
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 1 gm/kg
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 300,155,1993
TYPE OF TEST : Sperm Morphology
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 29750 mg/kg/5D (Intermittent)
REFERENCE :
CMMUAO Chemical Mutagens. Principles and Methods for Their Detection.
(Plenum Pub. Corp., 233 Spring St., New York, NY 10013) V.1- 1971-
Volume(issue)/page/year: 5,257,1978
TYPE OF TEST : Morphological transformation
TEST SYSTEM : Rodent - hamster Embryo
DOSE/DURATION : 8 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 343,7,1995
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Embryo
DOSE/DURATION : 50 mg/L
REFERENCE :
NATUAS Nature. (Nature Subscription Dept., POB 1018, Manasguan, NJ 08736)
V.1- 1869- Volume(issue)/page/year: 253,361,1975
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Fibroblast
DOSE/DURATION : 10 mg/L
REFERENCE :
CRNGDP Carcinogenesis (London). (Oxford Univ. Press, Pinkhill House,
Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1980-
Volume(issue)/page/year: 3,499,1982
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Bird - chicken Fibroblast
DOSE/DURATION : 50 mg/L
REFERENCE :
NATUAS Nature. (Nature Subscription Dept., POB 1018, Manasguan, NJ 08736)
V.1- 1869- Volume(issue)/page/year: 253,361,1975
*** REVIEWS ***
IARC Cancer Review:Animal Sufficient Evidence
IMSUDL IARC Monographs, Supplement. (WHO Publications Centre USA, 49
Sheridan Ave., Albany, NY 12210) No.1- 1979- Volume(issue)/page/year:
7,391,1987
IARC Cancer Review:Human No Adequate Data
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 7,391,1987
IARC Cancer Review:Group 2B
IMSUDL IARC Monographs, Supplement. (WHO Publications Centre USA, 49
Sheridan Ave., Albany, NY 12210) No.1- 1979- Volume(issue)/page/year:
7,56,1987
TOXICOLOGY REVIEW
LLOYA2 Lloydia. (Cincinnati, OH) V.1-41, 1938-78. For publisher
information, see JNPRDF. Volume(issue)/page/year: 38,21,1975
TOXICOLOGY REVIEW
32XPAD "Teratology," Berry, C.L., and D.E. Poswillo, eds., New York,
Springer, 1975 Volume(issue)/page/year: -,49,1975
TOXICOLOGY REVIEW
ARMIAZ Annual Review of Microbiology. (Annual Reviews, Inc., POB 10139,
Palo Alto, CA 94303) V.1- 1947- Volume(issue)/page/year: 26,279,1972
*** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA ***
NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA :
NOES - National Occupational Exposure Survey (1983)
NOES Hazard Code - X3685
No. of Facilities: 58 (estimated)
No. of Industries: 1
No. of Occupations: 1
No. of Employees: 1656 (estimated)
No. of Female Employees: 774 (estimated)
*** STATUS IN U.S. ***
EPA GENETOX PROGRAM 1988, Positive: Aspergillus-aneuploidy; Sperm
morphology-mouse
EPA GENETOX PROGRAM 1988, Positive/limited: Carcinogenicity-mouse/rat
EPA GENETOX PROGRAM 1988, Negative: S cerevisiae-homozygosis
EPA GENETOX PROGRAM 1988, Inconclusive: Aspergillus-recombination; Mammalian
micronucleus
EPA GENETOX PROGRAM 1988, Inconclusive: B subtilis rec assay; E coli polA
without S9
EPA GENETOX PROGRAM 1988, Inconclusive: Histidine reversion-Ames test
*** END OF RECORD ***