*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : XT1925000
CHEMICAL NAME : Toluene, 2,6-dinitro-
CAS REGISTRY NUMBER : 606-20-2
BEILSTEIN REFERENCE NO. : 2052046
REFERENCE : 4-05-00-00866 (Beilstein Handbook Reference)
LAST UPDATED : 199710
DATA ITEMS CITED : 34
MOLECULAR FORMULA : C7-H6-N2-O4
MOLECULAR WEIGHT : 182.15
WISWESSER LINE NOTATION : WNR B1 CNW
COMPOUND DESCRIPTOR : Tumorigen
Mutagen
Primary Irritant
SYNONYMS/TRADE NAMES :
* Benzene, 2-methyl-1,3-dinitro-
* 2,6-Dinitrotoluene
* 2,6-DNT
* 1-Methyl-2,6-dinitrobenzene
* 2-Methyl-1,3-dinitrobenzene
* RCRA waste number U106
*** HEALTH HAZARD DATA ***
** SKIN/EYE IRRITATION DATA **
TYPE OF TEST : Standard Draize test
ROUTE OF EXPOSURE : Administration onto the skin
SPECIES OBSERVED : Rodent - rabbit
DOSE/DURATION : 500 mg/24H
REACTION SEVERITY : Mild
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: AD-B011-150
** ACUTE TOXICITY DATA **
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 177 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: PB214-270
TYPE OF TEST : LC50 - Lethal concentration, 50 percent kill
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 240 mg/m3/6H
TOXIC EFFECTS :
Behavioral - general anesthetic
Lungs, Thorax, or Respiration - fibrosing alveolitis
Lungs, Thorax, or Respiration - changes in lung weight
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: OTS0533663
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 621 mg/kg
TOXIC EFFECTS :
Behavioral - somnolence (general depressed activity)
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: AD-A080-146
** OTHER MULTIPLE DOSE TOXICITY DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 13500 mg/kg/90D-I
TOXIC EFFECTS :
Blood - normocytic anemia
Blood - methemoglobinemia-carboxyhemoglobin
Related to Chronic Data - death
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
37,116,1976
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 27 gm/kg/90D-C
TOXIC EFFECTS :
Blood - normocytic anemia
Blood - methemoglobinemia-carboxyhemoglobin
Related to Chronic Data - death
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
37,116,1976
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Mammal - dog
DOSE/DURATION : 9 gm/kg/90D-C
TOXIC EFFECTS :
Blood - normocytic anemia
Blood - methemoglobinemia-carboxyhemoglobin
Related to Chronic Data - death
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
37,116,1976
** TUMORIGENIC DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 2555 mg/kg/1Y-C
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Liver - tumors
REFERENCE :
JJIND8 JNCI, Journal of the National Cancer Institute. (Washington, DC)
V.61-79, 1978-87. For publisher information, see JNCIEQ.
Volume(issue)/page/year: 79,1313,1987
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 5110 mg/kg/1Y-C
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Liver - tumors
REFERENCE :
PAACA3 Proceedings of the American Association for Cancer Research. (Waverly
Press, 428 E. Preston St., Baltimore, MD 21202) V.1- 1954-
Volume(issue)/page/year: 24,91,1983
** MUTATION DATA **
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 100 ug/plate
REFERENCE :
DTESD7 52 Vanderbilt Ave., New York, NY 10017) V.1-15, 1977-87.
Discontinued 52 Vanderbilt Ave., New York, NY 10017) V.1- 1977-
Volume(issue)/page/year: 2,249,1977
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 1 mg/plate
REFERENCE :
SAIGBL Sangyo Igaku. Japanese Journal of Industrial Health. (Nippon Sangyo
Eisei Igakkai, Kosu Eisei Bldg., 1-29-8, Shinjuku, Shinjuku-ku, Tokyo 160,
Japan) V.1- 1959- Volume(issue)/page/year: 29,34,1987
TYPE OF TEST : Morphological transformation
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Rodent - rat
DOSE/DURATION : 58800 ug/kg/3W (Continuous)
REFERENCE :
CRNGDP Carcinogenesis (London). (Oxford Univ. Press, Pinkhill House,
Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1980-
Volume(issue)/page/year: 7,1797,1986
TYPE OF TEST : DNA adduct
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - rat
DOSE/DURATION : 219 ug/kg
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 301,79,1993
TYPE OF TEST : DNA adduct
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Rodent - rat
DOSE/DURATION : 10 mg/kg
REFERENCE :
JTEHD6 Journal of Toxicology and Environmental Health. (Hemisphere Pub.,
1025 Vermont Ave., NW, Washington, DC 20005) V.1- 1975/76-
Volume(issue)/page/year: 11,555,1983
TYPE OF TEST : DNA damage
TEST SYSTEM : Rodent - rat Liver
DOSE/DURATION : 3 mmol/L
REFERENCE :
SinJF# Personal Communication from J.F. Sina, Merck Institute for
Therapeutic Research, West Point, PA 19486, Oct. 26, 1982
Volume(issue)/page/year: 26OCT1982
TYPE OF TEST : Mutation test systems - not otherwise specified
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Rodent - rat
DOSE/DURATION : 10 mg/kg
REFERENCE :
JTEHD6 Journal of Toxicology and Environmental Health. (Hemisphere Pub.,
1025 Vermont Ave., NW, Washington, DC 20005) V.1- 1975/76-
Volume(issue)/page/year: 11,555,1983
TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Rodent - rat Cells - not otherwise specified
DOSE/DURATION : 10 umol/L
REFERENCE :
CBTOE2 Cell Biology and Toxicology. (Princeton Scientific Pub., Inc., 301
N. Harrison St., CN 5279, Princeton, NJ 08540) V.1- 1984-
Volume(issue)/page/year: 7,111,1991
TYPE OF TEST : Unscheduled DNA synthesis
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Rodent - rat
DOSE/DURATION : 5 mg/kg
REFERENCE :
CRNGDP Carcinogenesis (London). (Oxford Univ. Press, Pinkhill House,
Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1980-
Volume(issue)/page/year: 3,241,1982
TYPE OF TEST : DNA inhibition
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - rat
DOSE/DURATION : 20 mg/kg/8W (Intermittent)
REFERENCE :
CBTOE2 Cell Biology and Toxicology. (Princeton Scientific Pub., Inc., 301
N. Harrison St., CN 5279, Princeton, NJ 08540) V.1- 1984-
Volume(issue)/page/year: 7,111,1991
TYPE OF TEST : Body fluid assay
TEST SYSTEM : Rodent - rat Bacteria - Salmonella typhimurium
DOSE/DURATION : 75 mg/kg
REFERENCE :
CALEDQ Cancer Letters (Shannon, Ireland). (Elsevier Scientific Pub. Ireland
Ltd., POB 85, Limerick, Ireland) V.1- 1975- Volume(issue)/page/year:
52,13,1990
TYPE OF TEST : Cytogenetic analysis
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Rodent - rat
DOSE/DURATION : 1260 mg/kg/6W (Continuous)
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: AD-A062-015
TYPE OF TEST : DNA adduct
ROUTE OF EXPOSURE : Administration onto the skin
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 192 umol/kg
REFERENCE :
CRNGDP Carcinogenesis (London). (Oxford Univ. Press, Pinkhill House,
Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1980-
Volume(issue)/page/year: 5,231,1984
TYPE OF TEST : Body fluid assay
TEST SYSTEM : Rodent - mouse Bacteria - Salmonella typhimurium
DOSE/DURATION : 75 mg/kg
REFERENCE :
CALEDQ Cancer Letters (Shannon, Ireland). (Elsevier Scientific Pub. Ireland
Ltd., POB 85, Limerick, Ireland) V.1- 1975- Volume(issue)/page/year:
52,13,1990
*** REVIEWS ***
IARC Cancer Review:Animal Sufficient Evidence
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 65,309,1996
IARC Cancer Review:Human Inadequate Evidence
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 65,309,1996
IARC Cancer Review:Group 2B
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 65,309,1996
*** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA ***
NIOSH RECOMMENDED EXPOSURE LEVEL (REL) :
NIOSH REL TO 2,6-DINITROTOLUENE-air:10H CA TWA 1.5 mg/m3 (Sk)
REFERENCE :
NIOSH* National Institute for Occupational Safety and Health, U.S. Dept. of
Health, Education, and Welfare, Reports and Memoranda.
Volume(issue)/page/year: DHHS #92-100,1992
*** STATUS IN U.S. ***
ATSDR TOXICOLOGY PROFILE
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: PB/90/171430/AS
EPA GENETOX PROGRAM 1988, Inconclusive: CHO gene mutation
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
On EPA IRIS database
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JUNE 1998
NIOSH CURRENT INTELLIGENCE BULLETIN #44
*** END OF RECORD ***