*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : YS5000000
CHEMICAL NAME : Urea,
1-(2-chloroethyl)-3-(4-methylcyclohexyl)-1-nitroso-
CAS REGISTRY NUMBER : 13909-09-6
LAST UPDATED : 199510
DATA ITEMS CITED : 44
MOLECULAR FORMULA : C10-H18-Cl-N3-O2
MOLECULAR WEIGHT : 247.76
WISWESSER LINE NOTATION : L6TJ AMVNNO&2G D1
COMPOUND DESCRIPTOR : Tumorigen
Drug
Mutagen
Human
Primary Irritant
SYNONYMS/TRADE NAMES :
* 1-(2-Chloroethyl)-3-(4-methyl-cyclohexyl)-1-nitrosourea
* 1-(2-Chloroethyl)-3-(trans-4-methyl-cyclohexyl)-1-nitrosourea
* N-(2-Chloroethyl)-N'-(trans-4-methylcyclohexyl)-N-nitrosourea
* ICIG 1110
* Lomustine, methyl-
* ME-Ccnu
* Methyl-ccnu
* trans-Methyl-ccnu
* NCI-C04955
* NSC-95441
* Semustine
* Urea, N-(2-chloroethyl)-N'-(4-methylcyclohexyl)-N-nitroso-
* Urea, 1-(2-chloroethyl)-3-(4-methylcyclohexyl)-1-nitroso-, trans-
*** HEALTH HAZARD DATA ***
** SKIN/EYE IRRITATION DATA **
TYPE OF TEST : Rinsed with water
ROUTE OF EXPOSURE : Administration onto the skin
SPECIES OBSERVED : Rodent - rabbit
DOSE/DURATION : 5 mg/24H
REFERENCE :
TXCYAC Toxicology. (Elsevier Scientific Pub. Ireland, Ltd., POB 85,
Limerick, Ireland) V.1- 1973- Volume(issue)/page/year: 14,117,1979
** ACUTE TOXICITY DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Human
DOSE/DURATION : 90 mg/kg
TOXIC EFFECTS :
Gastrointestinal - nausea or vomiting
Blood - leukopenia
Blood - thrombocytopenia
REFERENCE :
CCYPBY Cancer Chemotherapy Reports, Part 3. (Washington, DC) V.1-6,
1968-75. For publisher information, see CTRRDO. Volume(issue)/page/year:
4,257,1973
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Human
DOSE/DURATION : 6 mg/kg
TOXIC EFFECTS :
Gastrointestinal - nausea or vomiting
Blood - leukopenia
Blood - thrombocytopenia
REFERENCE :
CTRRDO Cancer Treatment Reports. (Washington, DC) V.60-71, 1976-87. For
publisher information, see JNCIEQ. Volume(issue)/page/year: 60,709,1976
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Human - child
DOSE/DURATION : 5550 mg/kg/30W-I
TOXIC EFFECTS :
Kidney, Ureter, Bladder - changes in both tubules and glomeruli
REFERENCE :
NEJMAG New England Journal of Medicine. (Massachusetts Medical Soc., 10
Shattuck St., Boston, MA 02115) V.198- 1928- Volume(issue)/page/year:
300,1200,1979
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Human - child
DOSE/DURATION : 37950 mg/kg/2Y-I
TOXIC EFFECTS :
Kidney, Ureter, Bladder - changes in both tubules and glomeruli
REFERENCE :
NEJMAG New England Journal of Medicine. (Massachusetts Medical Soc., 10
Shattuck St., Boston, MA 02115) V.198- 1928- Volume(issue)/page/year:
300,1200,1979
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 100 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
82,540,1986
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 49900 ug/kg
TOXIC EFFECTS :
Behavioral - changes in motor activity (specific assay)
Behavioral - withdrawal
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: PB269-473
TYPE OF TEST : LD10 - Lethal Dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 37 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
34,194,1974
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Unreported
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 30 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
EJMCA5 European Journal of Medicinal Chemistry--Chimie Therapeutique.
(Editions Scientifiques Elsevier, 29 rue Buffon, F-75005, Paris, France)
V.9- 1974- Volume(issue)/page/year: 12,397,1977
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Mammal - dog
DOSE/DURATION : 25 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
ACRSAJ Advances in Cancer Research. (Academic Press, Inc., 465 S. Lincoln
Dr., Troy, MO 63379) V.1- 1953- Volume(issue)/page/year: 16,273,1972
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Mammal - dog
DOSE/DURATION : 14 mg/kg
TOXIC EFFECTS :
Gastrointestinal - hypermotility, diarrhea
Blood - agranulocytosis
Nutritional and Gross Metabolic - body temperature decrease
REFERENCE :
CTRRDO Cancer Treatment Reports. (Washington, DC) V.60-71, 1976-87. For
publisher information, see JNCIEQ. Volume(issue)/page/year: 60,1559,1976
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Primate - monkey
DOSE/DURATION : 100 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
CCYPBY Cancer Chemotherapy Reports, Part 3. (Washington, DC) V.1-6,
1968-75. For publisher information, see CTRRDO. Volume(issue)/page/year:
4(3),13,1973
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Primate - monkey
DOSE/DURATION : 45 mg/kg
TOXIC EFFECTS :
Gastrointestinal - hypermotility, diarrhea
Blood - changes in bone marrow (not otherwise specified)
Blood - other changes
REFERENCE :
CTRRDO Cancer Treatment Reports. (Washington, DC) V.60-71, 1976-87. For
publisher information, see JNCIEQ. Volume(issue)/page/year: 60,1559,1976
** TUMORIGENIC DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Human
DOSE/DURATION : 22 mg/kg/60W-C
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Blood - leukemia
REFERENCE :
NEJMAG New England Journal of Medicine. (Massachusetts Medical Soc., 10
Shattuck St., Boston, MA 02115) V.198- 1928- Volume(issue)/page/year:
309,1079,1983
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 30 mg/kg/7W-I
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Lungs, Thorax, or Respiration - tumors
Skin and Appendages - tumors
REFERENCE :
CANCAR Cancer (Philadelphia). (Lippincott/Harper, Journal Fulfillment
Dept., 2350 Virginia Ave., Hagerstown, MD 21740) V.1- 1948-
Volume(issue)/page/year: 40,1935,1977
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 32 mg/kg/60W-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Gastrointestinal - tumors
REFERENCE :
DTESD7 52 Vanderbilt Ave., New York, NY 10017) V.1-15, 1977-87.
Discontinued 52 Vanderbilt Ave., New York, NY 10017) V.1- 1977-
Volume(issue)/page/year: 8,273,1980
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 117 mg/kg/26W-I
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Blood - leukemia
Blood - lymphoma, including Hodgkin's disease
REFERENCE :
CANCAR Cancer (Philadelphia). (Lippincott/Harper, Journal Fulfillment
Dept., 2350 Virginia Ave., Hagerstown, MD 21740) V.1- 1948-
Volume(issue)/page/year: 40,1935,1977
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 64 mg/kg/60W-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Gastrointestinal - tumors
REFERENCE :
DTESD7 52 Vanderbilt Ave., New York, NY 10017) V.1-15, 1977-87.
Discontinued 52 Vanderbilt Ave., New York, NY 10017) V.1- 1977-
Volume(issue)/page/year: 8,273,1980
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 127 mg/kg/60W-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Brain and Coverings - tumors
Gastrointestinal - tumors
REFERENCE :
DTESD7 52 Vanderbilt Ave., New York, NY 10017) V.1-15, 1977-87.
Discontinued 52 Vanderbilt Ave., New York, NY 10017) V.1- 1977-
Volume(issue)/page/year: 8,273,1980
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 203 mg/kg/48W-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Lungs, Thorax, or Respiration - tumors
Gastrointestinal - tumors
REFERENCE :
DTESD7 52 Vanderbilt Ave., New York, NY 10017) V.1-15, 1977-87.
Discontinued 52 Vanderbilt Ave., New York, NY 10017) V.1- 1977-
Volume(issue)/page/year: 8,273,1980
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Human - woman
DOSE/DURATION : 73 mg/kg/3Y-I
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Blood - leukemia
REFERENCE :
NEJMAG New England Journal of Medicine. (Massachusetts Medical Soc., 10
Shattuck St., Boston, MA 02115) V.198- 1928- Volume(issue)/page/year:
302,120,1980
** MUTATION DATA **
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 100 nmol/plate
REFERENCE :
JJIND8 JNCI, Journal of the National Cancer Institute. (Washington, DC)
V.61-79, 1978-87. For publisher information, see JNCIEQ.
Volume(issue)/page/year: 65,149,1980
TYPE OF TEST : DNA adduct
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 50 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 89,95,1981
TYPE OF TEST : Gene conversion and mitotic recombination
TEST SYSTEM : Yeast - Saccharomyces cerevisiae
DOSE/DURATION : 238 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 204,239,1988
TYPE OF TEST : DNA inhibition
TEST SYSTEM : Human Leukocyte
DOSE/DURATION : 1 umol/L
REFERENCE :
BBACAQ Biochimica et Biophysica Acta. (Elsevier Science Pub. B.V., POB 211,
1000 AE Amsterdam, Netherlands) V.1- 1947- Volume(issue)/page/year:
425,463,1976
TYPE OF TEST : Mutation test systems - not otherwise specified
TEST SYSTEM : Human Lymphocyte
DOSE/DURATION : 2 mg/L
REFERENCE :
TOLED5 Toxicology Letters. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1977- Volume(issue)/page/year: 44,153,1988
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Human Lymphocyte
DOSE/DURATION : 2 mg/L
REFERENCE :
TOLED5 Toxicology Letters. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1977- Volume(issue)/page/year: 44,153,1988
TYPE OF TEST : Sister chromatid exchange
TEST SYSTEM : Human Lymphocyte
DOSE/DURATION : 1 mg/L
REFERENCE :
TOLED5 Toxicology Letters. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1977- Volume(issue)/page/year: 44,153,1988
TYPE OF TEST : Micronucleus test
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 4 mg/kg
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 286,101,1993
TYPE OF TEST : Micronucleus test
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 5 mg/kg
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 286,101,1993
TYPE OF TEST : Unscheduled DNA synthesis
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 4 mg/kg
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
35,2420,1975
TYPE OF TEST : DNA inhibition
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 4 mg/kg
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
35,2420,1975
TYPE OF TEST : DNA inhibition
TEST SYSTEM : Rodent - mouse Cells - not otherwise specified
DOSE/DURATION : 10 umol/L
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
43,5837,1983
TYPE OF TEST : DNA inhibition
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - hamster
DOSE/DURATION : 40 mg/kg
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
34,1957,1974
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Ovary
DOSE/DURATION : 5 mg/L
REFERENCE :
NEOLA4 Neoplasma. (Karger-Libri, P.O. Box, CH-4009 Basel, Switzerland) V.4-
1957- Volume(issue)/page/year: 33,33,1986
TYPE OF TEST : Sister chromatid exchange
TEST SYSTEM : Rodent - hamster Ovary
DOSE/DURATION : 1 mg/L
REFERENCE :
NEOLA4 Neoplasma. (Karger-Libri, P.O. Box, CH-4009 Basel, Switzerland) V.4-
1957- Volume(issue)/page/year: 33,33,1986
TYPE OF TEST : DNA damage
TEST SYSTEM : Mammal - species unspecified Lymphocyte
DOSE/DURATION : 2 mmol/L
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
44,1887,1984
*** REVIEWS ***
IARC Cancer Review:Animal Limited Evidence
IMSUDL IARC Monographs, Supplement. (WHO Publications Centre USA, 49
Sheridan Ave., Albany, NY 12210) No.1- 1979- Volume(issue)/page/year:
7,150,1987
IARC Cancer Review:Human Sufficient Evidence
IMSUDL IARC Monographs, Supplement. (WHO Publications Centre USA, 49
Sheridan Ave., Albany, NY 12210) No.1- 1979- Volume(issue)/page/year:
7,150,1987
IARC Cancer Review:Group 1
IMSUDL IARC Monographs, Supplement. (WHO Publications Centre USA, 49
Sheridan Ave., Albany, NY 12210) No.1- 1979- Volume(issue)/page/year:
7,150,1987
*** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA ***
NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA :
NOES - National Occupational Exposure Survey (1983)
NOES Hazard Code - X1943
No. of Facilities: 8 (estimated)
No. of Industries: 1
No. of Occupations: 1
No. of Employees: 229 (estimated)
No. of Female Employees: 82 (estimated)
*** STATUS IN U.S. ***
NCI Carcinogenesis Studies (ipr);clear evidence:rat
CANCAR Cancer (Philadelphia). (Lippincott/Harper, Journal Fulfillment
Dept., 2350 Virginia Ave., Hagerstown, MD 21740) V.1- 1948-
Volume(issue)/page/year: 40,1935,77
NCI Carcinogenesis Studies (ipr);no evidence:mouse
CANCAR Cancer (Philadelphia). (Lippincott/Harper, Journal Fulfillment
Dept., 2350 Virginia Ave., Hagerstown, MD 21740) V.1- 1948-
Volume(issue)/page/year: 40,1935,77
NTP 7th Annual Report on Carcinogens, 1992 : known to be carcinogenic
*** END OF RECORD ***