*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : CZ7350000
CHEMICAL NAME : Benzene, m-dinitro-
CAS REGISTRY NUMBER : 99-65-0
LAST UPDATED : 199712
DATA ITEMS CITED : 37
MOLECULAR FORMULA : C6-H4-N2-O4
MOLECULAR WEIGHT : 168.12
WISWESSER LINE NOTATION : WNR CNW
COMPOUND DESCRIPTOR : Mutagen
Reproductive Effector
Human
Primary Irritant
SYNONYMS/TRADE NAMES :
* Benzene, 1,3-dinitro-
* Binitrobenzene
* m-Dinitrobenzene
* 1,3-Dinitrobenzene
* 2,4-Dinitrobenzene
* m-Dinitrobenzene
* 1,3-Dinitrobenzol
* Dwunitrobenzen
*** HEALTH HAZARD DATA ***
** SKIN/EYE IRRITATION DATA **
TYPE OF TEST : Standard Draize test
ROUTE OF EXPOSURE : Administration into the eye
SPECIES OBSERVED : Rodent - rabbit
DOSE/DURATION : 100 mg
REFERENCE :
ATDAEI Acute Toxicity Data. Journal of the American College of Toxicology,
Part B. (Mary Ann Liebert, Inc., 1651 Third Ave., New York, NY 10128) V.1-
1990- Volume(issue)/page/year: 1,168,1992
** ACUTE TOXICITY DATA **
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Human
DOSE/DURATION : 28 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
34ZIAG "Toxicology of Drugs and Chemicals," Deichmann, W.B., New York,
Academic Press, Inc., 1969 Volume(issue)/page/year: -,226,1969
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Administration onto the skin
SPECIES OBSERVED : Human - man
DOSE/DURATION : 4 mg/kg/2D-I
TOXIC EFFECTS :
Behavioral - changes in motor activity (specific assay)
Lungs, Thorax, or Respiration - cyanosis
REFERENCE :
LANCAO Lancet. (7 Adam St., London WC2N 6AD, UK) V.1- 1823-
Volume(issue)/page/year: 2,582,1901
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 59500 ug/kg
TOXIC EFFECTS :
Behavioral - somnolence (general depressed activity)
Lungs, Thorax, or Respiration - dyspnea
Skin and Appendages - hair
REFERENCE :
ATDAEI Acute Toxicity Data. Journal of the American College of Toxicology,
Part B. (Mary Ann Liebert, Inc., 1651 Third Ave., New York, NY 10128) V.1-
1990- Volume(issue)/page/year: 1,168,1992
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 28 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
AEPPAE Naunyn-Schmiedeberg's Archiv fuer Experimentelle Pathologie und
Pharmakologie. (Berlin, Ger.) V.110-253, 1925-66. For publisher
information, see NSAPCC. Volume(issue)/page/year: 207,446,1949
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 74700 ug/kg
TOXIC EFFECTS :
Behavioral - somnolence (general depressed activity)
Behavioral - tremor
Lungs, Thorax, or Respiration - respiratory stimulation
REFERENCE :
ATDAEI Acute Toxicity Data. Journal of the American College of Toxicology,
Part B. (Mary Ann Liebert, Inc., 1651 Third Ave., New York, NY 10128) V.1-
1990- Volume(issue)/page/year: 1,168,1992
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 200 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
JAPMA8 Journal of the American Pharmaceutical Association, Scientific
Edition. (Washington, DC) V.29-49, 1940-60. For publisher information, see
JPMSAE. Volume(issue)/page/year: 48,419,1959
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Mammal - dog
DOSE/DURATION : 600 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: AD-A066-307
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Mammal - dog
DOSE/DURATION : 10 mg/kg
TOXIC EFFECTS :
Peripheral Nerve and Sensation - spastic paralysis with or without sensory
change
Behavioral - convulsions or effect on seizure threshold
Blood - methemoglobinemia-carboxyhemoglobin
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: AD-A066-307
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Mammal - cat
DOSE/DURATION : 27 mg/kg
TOXIC EFFECTS :
Behavioral - coma
Lungs, Thorax, or Respiration - cyanosis
Blood - other hemolysis with or without anemia
REFERENCE :
LANCAO Lancet. (7 Adam St., London WC2N 6AD, UK) V.1- 1823-
Volume(issue)/page/year: 2,582,1901
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rabbit
DOSE/DURATION : 400 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: AD-A066-307
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Administration onto the skin
SPECIES OBSERVED : Rodent - rabbit
DOSE/DURATION : 1900 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
ATDAEI Acute Toxicity Data. Journal of the American College of Toxicology,
Part B. (Mary Ann Liebert, Inc., 1651 Third Ave., New York, NY 10128) V.1-
1990- Volume(issue)/page/year: 1,168,1992
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Bird - wild bird species
DOSE/DURATION : 42 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
21,315,1972
** OTHER MULTIPLE DOSE TOXICITY DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 156 mg/kg/90D-I
TOXIC EFFECTS :
Endocrine - changes in spleen weight
Blood - methemoglobinemia-carboxyhemoglobin
REFERENCE :
TOXID9 Toxicologist. (Soc. of Toxicology, Inc., 475 Wolf Ledge Parkway,
Akron, OH 44311) V.1- 1981- Volume(issue)/page/year: 30,200,1996
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 265 mg/kg/8W-C
TOXIC EFFECTS :
Blood - pigmented or nucleated red blood cells
Nutritional and Gross Metabolic - weight loss or decreased weight gain
Related to Chronic Data - changes in testicular weight
REFERENCE :
JTEHD6 Journal of Toxicology and Environmental Health. (Hemisphere Pub.,
1025 Vermont Ave., NW, Washington, DC 20005) V.1- 1975/76-
Volume(issue)/page/year: 7,829,1981
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 296 mg/kg/16W-C
TOXIC EFFECTS :
Endocrine - changes in spleen weight
Nutritional and Gross Metabolic - weight loss or decreased weight gain
Related to Chronic Data - changes in testicular weight
REFERENCE :
JTEHD6 Journal of Toxicology and Environmental Health. (Hemisphere Pub.,
1025 Vermont Ave., NW, Washington, DC 20005) V.1- 1975/76-
Volume(issue)/page/year: 7,829,1981
** REPRODUCTIVE DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE : 24 mg/kg
SEX/DURATION : male 1 day(s) pre-mating
TOXIC EFFECTS :
Reproductive - Paternal Effects - spermatogenesis (incl. genetic material,
sperm morphology, motility, and count)
Reproductive - Paternal Effects - testes, epididymis, sperm duct
REFERENCE :
FAATDF Fundamental and Applied Toxicology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1981- Volume(issue)/page/year:
14,284,1990
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE : 33600 ug/kg
SEX/DURATION : female 16 week(s) pre-mating
TOXIC EFFECTS :
Reproductive - Maternal Effects - ovaries, fallopian tubes
REFERENCE :
JTEHD6 Journal of Toxicology and Environmental Health. (Hemisphere Pub.,
1025 Vermont Ave., NW, Washington, DC 20005) V.1- 1975/76-
Volume(issue)/page/year: 7,829,1981
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE : 48 mg/kg
SEX/DURATION : male 1 day(s) pre-mating
TOXIC EFFECTS :
Reproductive - Fertility - male fertility index (e.g. # males impregnating
females per # males exposed to fertile nonpregnant females)
REFERENCE :
FAATDF Fundamental and Applied Toxicology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1981- Volume(issue)/page/year:
14,284,1990
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE : 90 mg/kg
SEX/DURATION : male 12 week(s) pre-mating
TOXIC EFFECTS :
Reproductive - Paternal Effects - spermatogenesis (incl. genetic material,
sperm morphology, motility, and count)
REFERENCE :
JTEHD6 Journal of Toxicology and Environmental Health. (Hemisphere Pub.,
1025 Vermont Ave., NW, Washington, DC 20005) V.1- 1975/76-
Volume(issue)/page/year: 19,477,1986
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE : 48 mg/kg
SEX/DURATION : male 1 day(s) pre-mating
TOXIC EFFECTS :
Reproductive - Paternal Effects - spermatogenesis (incl. genetic material,
sperm morphology, motility, and count)
Reproductive - Paternal Effects - testes, epididymis, sperm duct
REFERENCE :
JTEHD6 Journal of Toxicology and Environmental Health. (Hemisphere Pub.,
1025 Vermont Ave., NW, Washington, DC 20005) V.1- 1975/76-
Volume(issue)/page/year: 28,67,1989
** MUTATION DATA **
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 3300 ng/plate
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 2,531,1980
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 50 ug/plate
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 44,9,1977
TYPE OF TEST : Mutation test systems - not otherwise specified
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 12500 ug/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 272,91,1992
*** REVIEWS ***
ACGIH TLV-TWA 1.0 mg/m3 (0.15 ppm) (skin)
DTLVS* The Threshold Limit Values (TLVs) and Biological Exposure Indices
(BEIs) booklet issues by American Conference of Governmental Industrial
Hygienists (ACGIH), Cincinnati, OH, 1996 Volume(issue)/page/year:
TLV/BEI,1997
*** U.S. STANDARDS AND REGULATIONS ***
MSHA STANDARD-air:TWA 0.15 ppm (1 mg/m3) (skin)
DTLVS* The Threshold Limit Values (TLVs) and Biological Exposure Indices
(BEIs) booklet issues by American Conference of Governmental Industrial
Hygienists (ACGIH), Cincinnati, OH, 1996 Volume(issue)/page/year: 3,92,1971
OSHA PEL (Gen Indu):8H TWA 1 mg/m3 (skin)
CFRGBR Code of Federal Regulations. (U.S. Government Printing Office, Supt.
of Documents, Washington, DC 20402) Volume(issue)/page/year:
29,1910.1000,1994
OSHA PEL (Construc):8H TWA 1 mg/m3 (skin)
CFRGBR Code of Federal Regulations. (U.S. Government Printing Office, Supt.
of Documents, Washington, DC 20402) Volume(issue)/page/year:
29,1926.55,1994
OSHA PEL (Shipyard):8H TWA 1 mg/m3 (skin)
CFRGBR Code of Federal Regulations. (U.S. Government Printing Office, Supt.
of Documents, Washington, DC 20402) Volume(issue)/page/year:
29,1915.1000,1993
OSHA PEL (Fed Cont):8H TWA 1 mg/m3 (skin)
CFRGBR Code of Federal Regulations. (U.S. Government Printing Office, Supt.
of Documents, Washington, DC 20402) Volume(issue)/page/year:
41,50-204.50,1994
*** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA ***
NIOSH RECOMMENDED EXPOSURE LEVEL (REL) :
NIOSH REL TO m-DINITROBENZENE-air:10H TWA 1 mg/m3 (Sk)
REFERENCE :
NIOSH* National Institute for Occupational Safety and Health, U.S. Dept. of
Health, Education, and Welfare, Reports and Memoranda.
Volume(issue)/page/year: DHHS #92-100,1992
NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA :
NOHS - National Occupational Hazard Survey (1974)
NOHS Hazard Code - 27046
No. of Facilities: 184 (estimated)
No. of Industries: 2
No. of Occupations: 3
No. of Employees: 1754 (estimated)
NOES - National Occupational Exposure Survey (1983)
NOES Hazard Code - 27046
No. of Facilities: 41 (estimated)
No. of Industries: 2
No. of Occupations: 4
No. of Employees: 2489 (estimated)
No. of Female Employees: 1914 (estimated)
*** STATUS IN U.S. ***
EPA GENETOX PROGRAM 1988, Positive: Histidine reversion-Ames test
EPA TSCA Section 8(b) CHEMICAL INVENTORY
On EPA IRIS database
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JUNE 1998
*** END OF RECORD ***