*** CHEMICAL IDENTIFICATION *** RTECS NUMBER : DD0525000 CHEMICAL NAME : Benzidine, 3,3'-dichloro- CAS REGISTRY NUMBER : 91-94-1 BEILSTEIN REFERENCE NO. : 2108022 REFERENCE : 4-13-00-00384 (Beilstein Handbook Reference) LAST UPDATED : 199712 DATA ITEMS CITED : 53 MOLECULAR FORMULA : C12-H10-Cl2-N2 MOLECULAR WEIGHT : 253.14 WISWESSER LINE NOTATION : ZR BG DR DZ CG COMPOUND DESCRIPTOR : Tumorigen Mutagen Reproductive Effector SYNONYMS/TRADE NAMES : * (1,1'-Biphenyl)-4,4'-diamine, 3,3'-dichloro- * C.I. 23060 * Curithane C126 * 4,4'-Diamino-3,3'-dichlorobiphenyl * 4,4'-Diamino-3,3'-dichlorodiphenyl * 3,3'-Dichlorbenzidin * 3,3'-Dichlorobenzidina * Dichlorobenzidine * o,o'-Dichlorobenzidine * 3,3'-Dichlorobenzidine * Dichlorobenzidine base * 3,3'-Dichlorobenzidine * 3,3'-Dichloro-4,4'-biphenyldiamine * 3,3'-Dichlorobiphenyl-4,4'-diamine * 3,3'-Dichloro-4,4'-diaminobiphenyl * 3,3'-Dichloro-4,4'-diamino(1,1-biphenyl) * RCRA waste number U073 *** HEALTH HAZARD DATA *** ** TUMORIGENIC DATA ** TYPE OF TEST : TDLo - Lowest published toxic dose ROUTE OF EXPOSURE : Oral SPECIES OBSERVED : Rodent - rat DOSE/DURATION : 17 gm/kg/50W-C TOXIC EFFECTS : Tumorigenic - Carcinogenic by RTECS criteria Blood - tumors Skin and Appendages - tumors REFERENCE : TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year: 31,159,1975 TYPE OF TEST : TDLo - Lowest published toxic dose ROUTE OF EXPOSURE : Subcutaneous SPECIES OBSERVED : Rodent - rat DOSE/DURATION : 3600 mg/kg/61W-I TOXIC EFFECTS : Tumorigenic - equivocal tumorigenic agent by RTECS criteria Skin and Appendages - breast REFERENCE : VOONAW Voprosy Onkologii. Problems of Oncology. For English translation, see PONCAU. (V/O Mezhdunarodnaya Kniga, 113095 Moscow, USSR) V.1-10, 1928-37; V.1- 1955- Volume(issue)/page/year: 21(6),110,1975 TYPE OF TEST : TDLo - Lowest published toxic dose ROUTE OF EXPOSURE : Oral SPECIES OBSERVED : Rodent - mouse DOSE/DURATION : 5100 mg/kg/43W-I TOXIC EFFECTS : Tumorigenic - equivocal tumorigenic agent by RTECS criteria Liver - tumors REFERENCE : VOONAW Voprosy Onkologii. Problems of Oncology. For English translation, see PONCAU. (V/O Mezhdunarodnaya Kniga, 113095 Moscow, USSR) V.1-10, 1928-37; V.1- 1955- Volume(issue)/page/year: 5(5),524,1959 TYPE OF TEST : TDLo - Lowest published toxic dose ROUTE OF EXPOSURE : Subcutaneous SPECIES OBSERVED : Rodent - mouse DOSE/DURATION : 320 mg/kg female 15-21 day(s) after conception TOXIC EFFECTS : Tumorigenic - Carcinogenic by RTECS criteria Reproductive - Tumorigenic effects - transplacental tumorigenesis Lungs, Thorax, or Respiration - tumors REFERENCE : BEXBAN Bulletin of Experimental Biology and Medicine (English Translation). Translation of BEBMAE. (Plenum Pub. Corp., 233 Spring St., New York, NY 10013) V.41- 1956- Volume(issue)/page/year: 78,1402,1974 TYPE OF TEST : TDLo - Lowest published toxic dose ROUTE OF EXPOSURE : Subcutaneous SPECIES OBSERVED : Rodent - mouse DOSE/DURATION : 5200 mg/kg/47W-I TOXIC EFFECTS : Tumorigenic - equivocal tumorigenic agent by RTECS criteria Liver - tumors Tumorigenic - tumors at site of application REFERENCE : VOONAW Voprosy Onkologii. Problems of Oncology. For English translation, see PONCAU. (V/O Mezhdunarodnaya Kniga, 113095 Moscow, USSR) V.1-10, 1928-37; V.1- 1955- Volume(issue)/page/year: 5(5),524,1959 TYPE OF TEST : TDLo - Lowest published toxic dose ROUTE OF EXPOSURE : Oral SPECIES OBSERVED : Mammal - dog DOSE/DURATION : 17 gm/kg/7Y-I TOXIC EFFECTS : Tumorigenic - equivocal tumorigenic agent by RTECS criteria Liver - tumors Kidney, Ureter, Bladder - tumors REFERENCE : DUPON* E.I. Dupont de Nemours and Company, Technical Sheet. (1007 Market St., Wilmington, DE 19898) Volume(issue)/page/year: HL623-74,1974 TYPE OF TEST : TDLo - Lowest published toxic dose ROUTE OF EXPOSURE : Oral SPECIES OBSERVED : Rodent - hamster DOSE/DURATION : 176 gm/kg/70W-C TOXIC EFFECTS : Tumorigenic - equivocal tumorigenic agent by RTECS criteria Liver - tumors Kidney, Ureter, Bladder - tumors REFERENCE : PAACA3 Proceedings of the American Association for Cancer Research. (Waverly Press, 428 E. Preston St., Baltimore, MD 21202) V.1- 1954- Volume(issue)/page/year: 10,78,1969 TYPE OF TEST : TD - Toxic dose (other than lowest) ROUTE OF EXPOSURE : Oral SPECIES OBSERVED : Rodent - rat DOSE/DURATION : 20 gm/kg/52W-I TOXIC EFFECTS : Tumorigenic - equivocal tumorigenic agent by RTECS criteria Gastrointestinal - tumors Skin and Appendages - tumors REFERENCE : VOONAW Voprosy Onkologii. Problems of Oncology. For English translation, see PONCAU. (V/O Mezhdunarodnaya Kniga, 113095 Moscow, USSR) V.1-10, 1928-37; V.1- 1955- Volume(issue)/page/year: 5(5),524,1959 TYPE OF TEST : TD - Toxic dose (other than lowest) ROUTE OF EXPOSURE : Oral SPECIES OBSERVED : Rodent - rat DOSE/DURATION : 21 gm/kg/50W-C TOXIC EFFECTS : Tumorigenic - Carcinogenic by RTECS criteria Blood - tumors Skin and Appendages - tumors REFERENCE : TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year: 31,159,1975 TYPE OF TEST : TD - Toxic dose (other than lowest) ROUTE OF EXPOSURE : Subcutaneous SPECIES OBSERVED : Rodent - rat DOSE/DURATION : 7 gm/kg/43W-I TOXIC EFFECTS : Tumorigenic - equivocal tumorigenic agent by RTECS criteria Tumorigenic - tumors at site of application REFERENCE : VOONAW Voprosy Onkologii. Problems of Oncology. For English translation, see PONCAU. (V/O Mezhdunarodnaya Kniga, 113095 Moscow, USSR) V.1-10, 1928-37; V.1- 1955- Volume(issue)/page/year: 5(5),524,1959 ** MUTATION DATA ** TYPE OF TEST : Mutation in microorganisms TEST SYSTEM : Bacteria - Salmonella typhimurium DOSE/DURATION : 50 ug/plate REFERENCE : CALEDQ Cancer Letters (Shannon, Ireland). (Elsevier Scientific Pub. Ireland Ltd., POB 85, Limerick, Ireland) V.1- 1975- Volume(issue)/page/year: 4,21,1977 TYPE OF TEST : Mutation in microorganisms TEST SYSTEM : Bacteria - Salmonella typhimurium DOSE/DURATION : 10 nmol/plate REFERENCE : MUTAEX Mutagenesis. (Oxford Univ. Press, Pinkhill House, Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1986- Volume(issue)/page/year: 2,225,1987 TYPE OF TEST : Unscheduled DNA synthesis TEST SYSTEM : Human HeLa cell DOSE/DURATION : 100 nmol/L REFERENCE : CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year: 38,2621,1978 TYPE OF TEST : Morphological transformation TEST SYSTEM : Rodent - rat Embryo DOSE/DURATION : 5 mg/L REFERENCE : JNCIAM Journal of the National Cancer Institute. (Washington, DC) V.1-60, 1940-78. For publisher information, see JJIND8. Volume(issue)/page/year: 51,799,1973 TYPE OF TEST : Body fluid assay TEST SYSTEM : Rodent - rat Bacteria - Salmonella typhimurium DOSE/DURATION : 40 mg/kg REFERENCE : SAIGBL Sangyo Igaku. Japanese Journal of Industrial Health. (Nippon Sangyo Eisei Igakkai, Kosu Eisei Bldg., 1-29-8, Shinjuku, Shinjuku-ku, Tokyo 160, Japan) V.1- 1959- Volume(issue)/page/year: 23,426,1981 TYPE OF TEST : Micronucleus test ROUTE OF EXPOSURE : Oral TEST SYSTEM : Rodent - mouse DOSE/DURATION : 1 gm/kg REFERENCE : MUTAEX Mutagenesis. (Oxford Univ. Press, Pinkhill House, Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1986- Volume(issue)/page/year: 2,267,1987 TYPE OF TEST : Mutation test systems - not otherwise specified ROUTE OF EXPOSURE : Intraperitoneal TEST SYSTEM : Rodent - mouse DOSE/DURATION : 100 mg/kg REFERENCE : MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 319,19,1993 TYPE OF TEST : Cytogenetic analysis ROUTE OF EXPOSURE : Intraperitoneal TEST SYSTEM : Rodent - mouse DOSE/DURATION : 100 mg/kg REFERENCE : MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 319,19,1993 TYPE OF TEST : Morphological transformation TEST SYSTEM : Rodent - hamster Embryo DOSE/DURATION : 12500 ug/L REFERENCE : AETODY Advances in Modern Environmental Toxicology. (Senate Press, Inc., P.O. Box 252, Princeton Junction, NJ 08550) V.1- 1980- Volume(issue)/page/year: 1,241,1980 TYPE OF TEST : Morphological transformation TEST SYSTEM : Rodent - hamster Kidney DOSE/DURATION : 80 ug/L REFERENCE : BJCAAI British Journal of Cancer. (Macmillan Press Ltd., Houndmills, Basingstoke, Hants. RG21 2XS, UK) V.1- 1947- Volume(issue)/page/year: 37,873,1978 TYPE OF TEST : DNA adduct TEST SYSTEM : Mammal - species unspecified Lymphocyte DOSE/DURATION : 25500 nmol/L REFERENCE : CBINA8 Chemico-Biological Interactions. (Elsevier Scientific Pub. Ireland Ltd., POB 85, Limerick, Ireland) V.1- 1969- Volume(issue)/page/year: 38,369,1982 *** REVIEWS *** ACGIH TLV-Animal carcinogen, NO TWA (skin) DTLVS* The Threshold Limit Values (TLVs) and Biological Exposure Indices (BEIs) booklet issues by American Conference of Governmental Industrial Hygienists (ACGIH), Cincinnati, OH, 1996 Volume(issue)/page/year: TLV/BEI,1997 IARC Cancer Review:Animal Sufficient Evidence IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210) V.1- 1972- Volume(issue)/page/year: 4,49,1974 IARC Cancer Review:Animal Sufficient Evidence IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210) V.1- 1972- Volume(issue)/page/year: 29,239,1982 IARC Cancer Review:Human Inadequate Evidence IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210) V.1- 1972- Volume(issue)/page/year: 29,239,1982 IARC Cancer Review:Group 2B IMSUDL IARC Monographs, Supplement. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210) No.1- 1979- Volume(issue)/page/year: 7,193,1987 TOXICOLOGY REVIEW ADCSAJ Advances in Chemistry Series. (American Chemical Soc., Distribution Office Dept. 223, POB 57136, West End Stn., Washington, DC 20037) No.1- 1950- Volume(issue)/page/year: 13,271,1970 *** U.S. STANDARDS AND REGULATIONS *** MSHA STANDARD:skin-HUMAN CARCINOGEN, NO ASSIGNED TWA DTLVS* The Threshold Limit Values (TLVs) and Biological Exposure Indices (BEIs) booklet issues by American Conference of Governmental Industrial Hygienists (ACGIH), Cincinnati, OH, 1996 Volume(issue)/page/year: 3,77,1971 OSHA PEL (Gen Indu):see CFR 29,1910.1007 CFRGBR Code of Federal Regulations. (U.S. Government Printing Office, Supt. of Documents, Washington, DC 20402) Volume(issue)/page/year: 29,1910.1000,1994 OSHA PEL (Construc):see CFR 29,1926.1107 CFRGBR Code of Federal Regulations. (U.S. Government Printing Office, Supt. of Documents, Washington, DC 20402) Volume(issue)/page/year: 29,1926.55,1994 OSHA PEL (Shipyard):see CFR 29,1915.1007 CFRGBR Code of Federal Regulations. (U.S. Government Printing Office, Supt. of Documents, Washington, DC 20402) Volume(issue)/page/year: 29,1915.1000,1993 OSHA-cancer suspect agent CFRGBR Code of Federal Regulations. (U.S. Government Printing Office, Supt. of Documents, Washington, DC 20402) Volume(issue)/page/year: 29,1910.1007,1987 *** OCCUPATIONAL EXPOSURE LIMITS *** OEL-AUSTRALIA;Skin;Carcinogen JAN 1993 OEL-FRANCE;Carcinogen JAN 1993 OEL-GERMANY;Skin;Carcinogen JAN 1993 OEL-SWEDEN;Carcinogen JAN 1993 OEL-SWITZERLAND:TWA 0.1 mg/m3;Skin;Carcinogen JAN 1993 OEL-SWITZERLAND;Carcinogen JAN 1993 OEL IN BULGARIA, COLOMBIA, JORDAN, KOREA check ACGIH TLV OEL IN NEW ZEALAND, SINGAPORE, VIETNAM check ACGIH TLV *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH RECOMMENDED EXPOSURE LEVEL (REL) : NIOSH REL TO 3,3'-DICHLOROBENZIDINE-air:CA use 29 CFR 1910.1007 REFERENCE : NIOSH* National Institute for Occupational Safety and Health, U.S. Dept. of Health, Education, and Welfare, Reports and Memoranda. Volume(issue)/page/year: DHHS #92-100,1992 *** STATUS IN U.S. *** ATSDR TOXICOLOGY PROFILE NTIS** National Technical Information Service. (Springfield, VA 22161) Formerly U.S. Clearinghouse for Scientific & Technical Information. Volume(issue)/page/year: PB/90/171455/AS EPA GENETOX PROGRAM 1988, Positive: Carcinogenicity-mouse/rat EPA GENETOX PROGRAM 1988, Positive: Cell transform.-RLV F344 rat embryo EPA GENETOX PROGRAM 1988, Positive: Cell transform.-SA7/SHE; Histidine reversion-Ames test EPA GENETOX PROGRAM 1988, Inconclusive: In vitro UDS-human fibroblast EPA TSCA Section 8(b) CHEMICAL INVENTORY EPA TSCA Section 8(d) unpublished health/safety studies On EPA IRIS database EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JUNE 1998 NIOSH Analytical Method, 1994: 3,3'-Dichlorobenzidine, 5509 NTP 7th Annual Report on Carcinogens, 1992 : anticipated to be carcinogen OSHA ANALYTICAL METHOD #ID-65 *** END OF RECORD ***