*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : DJ4200000
CHEMICAL NAME : Benzo(e)pyrene
CAS REGISTRY NUMBER : 192-97-2
BEILSTEIN REFERENCE NO. : 1911334
REFERENCE : 4-05-00-02689 (Beilstein Handbook Reference)
LAST UPDATED : 199710
DATA ITEMS CITED : 40
MOLECULAR FORMULA : C20-H12
MOLECULAR WEIGHT : 252.32
WISWESSER LINE NOTATION : L D6666 B6 2AB TJ
COMPOUND DESCRIPTOR : Tumorigen
Mutagen
Reproductive Effector
SYNONYMS/TRADE NAMES :
* 1,2-Benzopyrene
* 4,5-Benzopyrene
* 1,2-Benzpyrene
* B(e)P
*** HEALTH HAZARD DATA ***
** TUMORIGENIC DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 360 mg/kg/43W-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Gastrointestinal - tumors
REFERENCE :
VRRAAT Vestnik Rentgenologii i Radiologii. Bulletin of Roentgenology and
Radiology. (V/O Mezhdunarodnaya Kniga, 113095 Moscow, USSR) V.1- 1920-
Volume(issue)/page/year: 20,276,1938
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Administration onto the skin
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 240 mg/kg/30W-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Skin and Appendages - tumors
REFERENCE :
CALEDQ Cancer Letters (Shannon, Ireland). (Elsevier Scientific Pub. Ireland
Ltd., POB 85, Limerick, Ireland) V.1- 1975- Volume(issue)/page/year:
7,51,1979
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 160 mg/kg
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Tumorigenic - facilitates action of known carcinogen
REFERENCE :
AJCAA7 American Journal of Cancer. (New York, NY) V.15-40, 1931-40. For
publisher information, see CNREA8. Volume(issue)/page/year: 27,474,1936
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - guinea pig
DOSE/DURATION : 140 mg/kg/37W-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Tumorigenic - facilitates action of known carcinogen
REFERENCE :
AJCAA7 American Journal of Cancer. (New York, NY) V.15-40, 1931-40. For
publisher information, see CNREA8. Volume(issue)/page/year: 27,474,1936
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Administration onto the skin
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 516 mg/kg/43W-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Skin and Appendages - tumors
REFERENCE :
CANCAR Cancer (Philadelphia). (Lippincott/Harper, Journal Fulfillment
Dept., 2350 Virginia Ave., Hagerstown, MD 21740) V.1- 1948-
Volume(issue)/page/year: 12,1079,1959
** REPRODUCTIVE DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE : 300 mg/kg
SEX/DURATION : female 8 day(s) after conception
TOXIC EFFECTS :
Reproductive - Fertility - post-implantation mortality (e.g. dead and/or
resorbed implants per total number of implants)
Reproductive - Specific Developmental Abnormalities - musculoskeletal system
REFERENCE :
SEIJBO Senten Ijo. Congenital Anomalies. (Nippon Senten Ijo Gakkai, c/o
Kinki Daigaku Igakubu Kaibagaku Kyoshitsu, 380 Nishiyama, Sayama-cho,
Mirami-Kawachi-gun, Osaka-fu, Japan) V.1-26, 1960-86. For publisher
information, see CGANE7. Volume(issue)/page/year: 21,97,1981
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE : 150 mg/kg
SEX/DURATION : female 8 day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death,
e.g., stunted fetus)
REFERENCE :
SEIJBO Senten Ijo. Congenital Anomalies. (Nippon Senten Ijo Gakkai, c/o
Kinki Daigaku Igakubu Kaibagaku Kyoshitsu, 380 Nishiyama, Sayama-cho,
Mirami-Kawachi-gun, Osaka-fu, Japan) V.1-26, 1960-86. For publisher
information, see CGANE7. Volume(issue)/page/year: 21,97,1981
** MUTATION DATA **
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 1 ug/plate
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 6(Suppl
2),1,1984
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Yeast - Saccharomyces cerevisiae
DOSE/DURATION : 100 mg/L
REFERENCE :
MUTAEX Mutagenesis. (Oxford Univ. Press, Pinkhill House, Southfield Road,
Eynsham, Oxford OX8 1JJ, UK) V.1- 1986- Volume(issue)/page/year: 4,335,1989
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Human Lymphocyte
DOSE/DURATION : 2 mg/L
REFERENCE :
MUTAEX Mutagenesis. (Oxford Univ. Press, Pinkhill House, Southfield Road,
Eynsham, Oxford OX8 1JJ, UK) V.1- 1986- Volume(issue)/page/year: 4,335,1989
TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Human HeLa cell
DOSE/DURATION : 1 umol/L
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
38,2621,1978
TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Human Cells - not otherwise specified
DOSE/DURATION : 12 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 130,127,1984
TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Human Lymphocyte
DOSE/DURATION : 8 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 371,123,1996
TYPE OF TEST : DNA damage
TEST SYSTEM : Rodent - rat Liver
DOSE/DURATION : 10 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 368,59,1996
TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Rodent - rat Liver
DOSE/DURATION : 7900 nmol/L
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
42,3010,1982
TYPE OF TEST : Micronucleus test
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 100 mg/kg
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 192,185,1987
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Rodent - mouse Lymphocyte
DOSE/DURATION : 625 ug/L
REFERENCE :
EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E.
11th St., New York, NY 10003) V.10- 1987- Volume(issue)/page/year:
12(Suppl 13),103,1988
TYPE OF TEST : Morphological transformation
TEST SYSTEM : Rodent - mouse Embryo
DOSE/DURATION : 10 mg/L
REFERENCE :
EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E.
11th St., New York, NY 10003) V.10- 1987- Volume(issue)/page/year:
12,21,1988
TYPE OF TEST : DNA adduct
ROUTE OF EXPOSURE : Administration onto the skin
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 192 umol/kg
REFERENCE :
CRNGDP Carcinogenesis (London). (Oxford Univ. Press, Pinkhill House,
Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1980-
Volume(issue)/page/year: 5,231,1984
TYPE OF TEST : Morphological transformation
TEST SYSTEM : Rodent - hamster Kidney
DOSE/DURATION : 25 ug/L
REFERENCE :
TOLED5 Toxicology Letters. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1977- Volume(issue)/page/year: 7,143,1980
TYPE OF TEST : Morphological transformation
TEST SYSTEM : Rodent - hamster Embryo
DOSE/DURATION : 10 mg/L
REFERENCE :
JNCIAM Journal of the National Cancer Institute. (Washington, DC) V.1-60,
1940-78. For publisher information, see JJIND8. Volume(issue)/page/year:
42,867,1969
TYPE OF TEST : DNA damage
TEST SYSTEM : Rodent - hamster Lung
DOSE/DURATION : 100 umol/L/4H
REFERENCE :
BBRCA9 Biochemical and Biophysical Research Communications. (Academic
Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1959-
Volume(issue)/page/year: 72,732,1976
TYPE OF TEST : Sister chromatid exchange
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - hamster
DOSE/DURATION : 900 mg/kg/24H
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 66,65,1979
TYPE OF TEST : Sister chromatid exchange
TEST SYSTEM : Rodent - hamster Ovary
DOSE/DURATION : 1 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 53,284,1978
*** REVIEWS ***
IARC Cancer Review:Animal Limited Evidence
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 3,137,1973
IARC Cancer Review:Animal Inadequate Evidence
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 32,225,1983
IARC Cancer Review:Human No Adequate Data
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 32,225,1983
IARC Cancer Review:Group 3
IMSUDL IARC Monographs, Supplement. (WHO Publications Centre USA, 49
Sheridan Ave., Albany, NY 12210) No.1- 1979- Volume(issue)/page/year:
7,56,1987
*** STATUS IN U.S. ***
EPA GENETOX PROGRAM 1988, Positive: Histidine reversion-Ames test
EPA GENETOX PROGRAM 1988, Positive: In vitro UDS-human fibroblast
EPA GENETOX PROGRAM 1988, Negative: Cell transform.-BALB/c-3T3; SHE-clonal
assay
EPA GENETOX PROGRAM 1988, Negative: Cell transform.-RLV F344 rat embryo; E
coli polA with S9
EPA GENETOX PROGRAM 1988, Negative: V79 cell culture-gene mutation; S
cerevisiae-homozygosis
EPA GENETOX PROGRAM 1988, Inconclusive: Carcinogenicity-mouse/rat
EPA GENETOX PROGRAM 1988, Inconclusive: L5178Y cells in vitro-TK test; E
coli polA without S9
EPA GENETOX PROGRAM 1988, Inconclusive: In vivo SCE-nonhuman
EPA TSCA Section 8(d) unpublished health/safety studies
On EPA IRIS database
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JUNE 1998
NIOSH Analytical Method, 1994: Polynuclear aromatic hydrocarbons by HPLC,
5506; by GC, 5515
*** END OF RECORD ***