*** CHEMICAL IDENTIFICATION ***

RTECS NUMBER            : DU3000000
CHEMICAL NAME           : (delta(sup 2,2')-Biindoline)-5,5'-disulfonic acid,
                          3,3'-dioxo-, disodium salt
CAS REGISTRY NUMBER     : 860-22-0
LAST UPDATED            : 199709
DATA ITEMS CITED        : 16
MOLECULAR FORMULA       : C16-H8-N2-O8-S2.2Na
MOLECULAR WEIGHT        : 466.36
WISWESSER LINE NOTATION : T56 BMYVJ GSWO CU- CT56 BMYVJ GSWO &-NA- 2
COMPOUND DESCRIPTOR     : Tumorigen
                          Drug
                          Mutagen
SYNONYMS/TRADE NAMES :
   * Acid Blue W
   * Acid Leather Blue IC
   * A.F. Blue No. 2
   * Airedale Blue IN
   * Amacid Brilliant Blue
   * Aniline Carmine powder
   * Atul Indigo Carmine
   * 1311 Blue
   * 12070 Blue
   * Bucacid indigotine B
   * Canacert Indigo Carmine
   * Carmine Blue (biological stain)
   * C.I. 73015
   * C.I. 75781
   * C.I. Acid Blue 74
   * C.I. Food Blue 1
   * C.I. Food Blue 1, disodium salt
   * Cilefa Blue R
   * C.I. Natural Blue 2
   * Disodium indigo-5,5-disulfonate
   * Disodium salt of 1-indigotin-S,S'-disulphonic acid
   * Dolkwal Indigo Carmine
   * E 132
   * Edicol Supra Blue X
   * FD and C Blue No. 2
   * Food Blue 2
   * Grape Blue A Geigy
   * HD Indigo Carmine
   * HD Indigo Carmine Supra
   * Hexacert Blue No. 2
   * Hexacol Indigo Carmine Supra
   * Indigo Carmine
   * Indigo Carmine A
   * Indigo Carmine AC
   * Indigo Carmine (biological stain)
   * Indigo Carmine BP
   * Indigo Carmine Conc. FQ
   * Indigo Carmine disodium salt
   * Indigo Carmine powder
   * Indigo Carmine X
   * Indigo disulfonate (biological stain)
   * Indigo extract
   * Indigo-karmin
   * Indigotin I
   * 5,5'-Indigotindisulfonic acid
   * Indigotin-5,5'-disulfonic acid disodium salt
   * Indigotine
   * Indigotine B
   * Indigotine Blue LZ
   * Indigotine conc. powder
   * Indigotine disodium salt
   * Indigotine Extra Pure A
   * Indigotine I
   * Indigotine Lake
   * Indigotine N
   * Indocarmine F
   * Intense Blue
   * L-Blau 2
   * Maple Indigo Carmine
   * Mitsui Indigo Carmine
   * Modr Kysela 74
   * Modr Pigment 63
   * Modr Potravinarska 1
   * Murabba
   * Sachsischblau
   * San-ei Indigo Carmine
   * Schultz Nr. 1309
   * Sodium indigotindisulfonate
   * Sodium 5,5'-indigotidisulfonate
   * Soluble indigo
   * Sumitomo Wool Blue SBC
   * Usacert Blue No. 2
 
                       *** HEALTH HAZARD DATA ***
 
                        ** ACUTE TOXICITY DATA **
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Oral
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 2 gm/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   SCPHA4 Scientia Pharmaceutica.  (Oesterreichische
   Apotheker-Verlagsgesellschaft MBH, Spitalgasse 31, 1094 Vienna 9, Austria)
   V.1-    1930-  Volume(issue)/page/year: 47,39,1979
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 93 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   NIIRDN Drugs in Japan (Ethical Drugs).  (Yakugyo Jiho Co., Ltd., Tokyo,
   Japan)  Volume(issue)/page/year: 6,83,1982
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Oral
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 2500 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   SCPHA4 Scientia Pharmaceutica.  (Oesterreichische
   Apotheker-Verlagsgesellschaft MBH, Spitalgasse 31, 1094 Vienna 9, Austria)
   V.1-    1930-  Volume(issue)/page/year: 47,39,1979
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Subcutaneous
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 405 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   SCPHA4 Scientia Pharmaceutica.  (Oesterreichische
   Apotheker-Verlagsgesellschaft MBH, Spitalgasse 31, 1094 Vienna 9, Austria)
   V.1-    1930-  Volume(issue)/page/year: 47,39,1979
 
                          ** TUMORIGENIC DATA **
 
TYPE OF TEST            : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE       : Subcutaneous
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 9 gm/kg/2Y-I
TOXIC EFFECTS :
   Tumorigenic - neoplastic by RTECS criteria
   Tumorigenic - tumors at site of application
REFERENCE :
   TXAPA9 Toxicology and Applied Pharmacology.  (Academic Press, Inc., 1 E.
   First St., Duluth, MN 55802) V.1-    1959-  Volume(issue)/page/year:
   8,29,1966
 
                            ** MUTATION DATA **
 
TYPE OF TEST            : Cytogenetic analysis
ROUTE OF EXPOSURE       : Oral
TEST SYSTEM             : Rodent - mouse
DOSE/DURATION           : 5400 mg/kg/90D (Continuous)
REFERENCE :
   CYTBAI Cytobios. (Faculty Press, 88 Regent St., Cambridge, UK)  V.1- 1969- 
   Volume(issue)/page/year: 54,25,1988
 
TYPE OF TEST            : Cytogenetic analysis
ROUTE OF EXPOSURE       : Oral
TEST SYSTEM             : Rodent - mouse
DOSE/DURATION           : 60 mg/kg/30D (Continuous)
REFERENCE :
   CALEDQ Cancer Letters (Shannon, Ireland).  (Elsevier Scientific Pub. Ireland
   Ltd., POB 85, Limerick, Ireland)  V.1-    1975- Volume(issue)/page/year:
   30,315,1986
 
TYPE OF TEST            : Sister chromatid exchange
ROUTE OF EXPOSURE       : Intraperitoneal
TEST SYSTEM             : Rodent - mouse
DOSE/DURATION           : 25 mg/kg
REFERENCE :
   CALEDQ Cancer Letters (Shannon, Ireland).  (Elsevier Scientific Pub. Ireland
   Ltd., POB 85, Limerick, Ireland)  V.1-    1975- Volume(issue)/page/year:
   52,33,1990
 
TYPE OF TEST            : Cytogenetic analysis
TEST SYSTEM             : Rodent - hamster Fibroblast
DOSE/DURATION           : 12 gm/L
REFERENCE :
   FCTOD7 Food and Chemical Toxicology.  (Pergamon Press Inc., Maxwell House,
   Fairview Park, Elmsford, NY 10523)  V.20-    1982- Volume(issue)/page/year:
   22,623,1984
 
TYPE OF TEST            : Cytogenetic analysis
TEST SYSTEM             : Rodent - hamster Ovary
DOSE/DURATION           : 20 umol/L/5H (Continuous)
REFERENCE :
   ENMUDM Environmental Mutagenesis.  (New York, NY)  V.1-9, 1979-87.  For
   publisher information, see EMMUEG.  Volume(issue)/page/year: 1,27,1979

         *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA ***

NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA :
   NOHS - National Occupational Hazard Survey (1974)
     NOHS Hazard Code - 84752
       No. of Facilities: 314 (estimated)
       No. of Industries: 5
       No. of Occupations: 6
       No. of Employees: 1736 (estimated)
   
   NOES - National Occupational Exposure Survey (1983)
     NOES Hazard Code - 84752
       No. of Facilities: 393 (estimated)
       No. of Industries: 7
       No. of Occupations: 28
       No. of Employees: 9691 (estimated)
       No. of Female Employees: 3035 (estimated)

                          *** STATUS IN U.S. ***
 
   EPA GENETOX PROGRAM 1988, Negative: Histidine reversion-Ames test
   
   EPA GENETOX PROGRAM 1988, Negative: S cerevisiae gene conversion
   
   EPA GENETOX PROGRAM 1988, Inconclusive: B subtilis rec assay
   
   EPA TSCA Section 8(b) CHEMICAL INVENTORY

 
                            *** END OF RECORD ***