*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : KC0180000
CHEMICAL NAME : Epipodophyllotoxin, 4'-demethyl-,
9-(4,6-O-2-thenylidene-beta-D-glucopyranoside)
CAS REGISTRY NUMBER : 29767-20-2
OTHER CAS REGISTRY NOS. : 31514-29-1
35317-44-3
23362-13-2
LAST UPDATED : 199801
DATA ITEMS CITED : 44
MOLECULAR FORMULA : C32-H32-O13-S
MOLECULAR WEIGHT : 656.70
COMPOUND DESCRIPTOR : Drug
Mutagen
Reproductive Effector
Human
SYNONYMS/TRADE NAMES :
* 4'-Demethylepipodophyllotoxin
9-(4,6-O-2-thenylidene-beta-D-glucopyranoside)
* 4'-Demethyl-epipodophyllotoxin-beta-D-thenylidene-glucoside
* 4'-Demethyl
1-O-(4,6-O,O-(2-thenylidene)-beta-D-glucopyranosyl)epipodophyllotoxin
* Epipodophyllotoxin-beta-D-thenylidene-glucoside, 4'-demethyl-
* EPT
* NSC-122819
* Teniposide
* Vehem
* VM-26
* Vumon
*** HEALTH HAZARD DATA ***
** ACUTE TOXICITY DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Human
DOSE/DURATION : 9579 mg/kg
TOXIC EFFECTS :
Behavioral - anorexia (human)
Gastrointestinal - nausea or vomiting
Skin and Appendages - hair
REFERENCE :
EJCAAH European Journal of Cancer. (Oxford, UK) V.1-17(6), 1965-1981. For
publisher information, see EJCODS. Volume(issue)/page/year: 14,1395,1978
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Human
DOSE/DURATION : 26 mg/kg/10D-I
TOXIC EFFECTS :
Blood - agranulocytosis
Blood - aplastic anemia
Blood - changes in bone marrow (not otherwise specified)
REFERENCE :
CANCAR Cancer (Philadelphia). (Lippincott/Harper, Journal Fulfillment
Dept., 2350 Virginia Ave., Hagerstown, MD 21740) V.1- 1948-
Volume(issue)/page/year: 34,985,1974
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Human
DOSE/DURATION : 132 mg/kg/7W-I
TOXIC EFFECTS :
Gastrointestinal - nausea or vomiting
Blood - leukopenia
REFERENCE :
CTRRDO Cancer Treatment Reports. (Washington, DC) V.60-71, 1976-87. For
publisher information, see JNCIEQ. Volume(issue)/page/year: 63,7,1979
TYPE OF TEST : LD17 - Lethal Dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 50 mg/kg
TOXIC EFFECTS :
Tumorigenic - active as anti-cancer agent
REFERENCE :
CTRRDO Cancer Treatment Reports. (Washington, DC) V.60-71, 1976-87. For
publisher information, see JNCIEQ. Volume(issue)/page/year: 60,1127,1976
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 15 mg/kg
TOXIC EFFECTS :
Lungs, Thorax, or Respiration - dyspnea
Gastrointestinal - hypermotility, diarrhea
Blood - leukopenia
REFERENCE :
PESTD5 Proceedings of the European Society of Toxicology. (Amsterdam,
Netherlands) V.16-18, 1975-77. Discontinued. Volume(issue)/page/year:
17,171,1976
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 29570 ug/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
NCISP* National Cancer Institute Screening Program Data Summary,
Developmental Therapeutics Program. (Bethesda, MD 20205)
Volume(issue)/page/year: JAN1986
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 31560 ug/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
NCISP* National Cancer Institute Screening Program Data Summary,
Developmental Therapeutics Program. (Bethesda, MD 20205)
Volume(issue)/page/year: JAN1986
** OTHER MULTIPLE DOSE TOXICITY DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 168 mg/kg/4W-I
TOXIC EFFECTS :
Related to Chronic Data - death
REFERENCE :
PESTD5 Proceedings of the European Society of Toxicology. (Amsterdam,
Netherlands) V.16-18, 1975-77. Discontinued. Volume(issue)/page/year:
17,171,1976
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 18 mg/kg/7D-I
TOXIC EFFECTS :
Gastrointestinal - other changes
Liver - changes in liver weight
Related to Chronic Data - death
REFERENCE :
CCROBU Cancer Chemotherapy Reports, Part 1. (Washington, DC) V.52(6)-59,
1968-75. For publisher information, see CTRRDO. Volume(issue)/page/year:
57,165,1973
** REPRODUCTIVE DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE : 1 mg/kg
SEX/DURATION : female 7 day(s) after conception
TOXIC EFFECTS :
Reproductive - Specific Developmental Abnormalities - Central Nervous System
Reproductive - Specific Developmental Abnormalities - cardiovascular
(circulatory) system
Reproductive - Specific Developmental Abnormalities - respiratory system
REFERENCE :
TJADAB Teratology, The International Journal of Abnormal Development. (Alan
R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.1- 1968-
Volume(issue)/page/year: 18,31,1978
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE : 1 mg/kg
SEX/DURATION : female 7 day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death,
e.g., stunted fetus)
Reproductive - Effects on Embryo or Fetus - fetal death
Reproductive - Specific Developmental Abnormalities - musculoskeletal system
REFERENCE :
TJADAB Teratology, The International Journal of Abnormal Development. (Alan
R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.1- 1968-
Volume(issue)/page/year: 18,31,1978
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE : 1 mg/kg
SEX/DURATION : female 6 day(s) after conception
TOXIC EFFECTS :
Reproductive - Maternal Effects - oogenesis
REFERENCE :
TJADAB Teratology, The International Journal of Abnormal Development. (Alan
R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.1- 1968-
Volume(issue)/page/year: 18,31,1978
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE : 1 mg/kg
SEX/DURATION : female 8 day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Embryo or Fetus - cytological changes (including
somatic cell genetic material)
REFERENCE :
TJADAB Teratology, The International Journal of Abnormal Development. (Alan
R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.1- 1968-
Volume(issue)/page/year: 18,31,1978
** MUTATION DATA **
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 100 ug/plate
REFERENCE :
TCMUD8 Teratogenesis, Carcinogenesis, and Mutagenesis. (Alan R. Liss, Inc.,
41 E. 11th St., New York, NY 10003) V.1- 1980- Volume(issue)/page/year:
5,319,1985
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 100 ug/plate
REFERENCE :
JTSCDR Journal of Toxicological Sciences. (Japanese Soc. of Toxicological
Sciences, 4th Floor, Gakkai Center Bldg., 4-16, Yayoi 2-chome, Bunkyo-ku,
Tokyo 113, Japan) V.1- 1976- Volume(issue)/page/year: 11(Suppl
1),301,1986
TYPE OF TEST : DNA repair
TEST SYSTEM : Bacteria - Bacillus subtilis
DOSE/DURATION : 10 ug/disc
REFERENCE :
JTSCDR Journal of Toxicological Sciences. (Japanese Soc. of Toxicological
Sciences, 4th Floor, Gakkai Center Bldg., 4-16, Yayoi 2-chome, Bunkyo-ku,
Tokyo 113, Japan) V.1- 1976- Volume(issue)/page/year: 11(Suppl
1),301,1986
TYPE OF TEST : DNA damage
TEST SYSTEM : Human Cells - not otherwise specified
DOSE/DURATION : 500 nmol/L
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
45,3106,1985
TYPE OF TEST : DNA damage
TEST SYSTEM : Human HeLa cell
DOSE/DURATION : 100 umol/L
REFERENCE :
BICHAW Biochemistry. (American Chemical Soc., Distribution Office Dept.
223, POB 57136, West End Stn., Washington, DC 20037) V.1- 1962-
Volume(issue)/page/year: 15,5443,1976
TYPE OF TEST : DNA damage
TEST SYSTEM : Human Mammary gland
DOSE/DURATION : 1500 nmol/L
REFERENCE :
PAACA3 Proceedings of the American Association for Cancer Research. (Waverly
Press, 428 E. Preston St., Baltimore, MD 21202) V.1- 1954-
Volume(issue)/page/year: 27,278,1986
TYPE OF TEST : DNA damage
TEST SYSTEM : Human Cells - not otherwise specified
DOSE/DURATION : 10 nmol/L/1H
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
46,3809,1986
TYPE OF TEST : DNA inhibition
TEST SYSTEM : Human Cells - not otherwise specified
DOSE/DURATION : 10 umol/L
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
46,2929,1986
TYPE OF TEST : DNA inhibition
TEST SYSTEM : Human Lymphocyte
DOSE/DURATION : 1 umol/L
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
35,99,1975
TYPE OF TEST : Mutation test systems - not otherwise specified
TEST SYSTEM : Human Lymphocyte
DOSE/DURATION : 1 umol/L
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
35,99,1975
TYPE OF TEST : Mutation test systems - not otherwise specified
TEST SYSTEM : Human Lymphocyte
DOSE/DURATION : 5 mg/L
REFERENCE :
CYTOAN Cytologia. (Japan Pub. Trading Co. (USA), 1255 Howard St., San
Francisco, CA 94103) V.1- 1929- Volume(issue)/page/year: 52,161,1987
TYPE OF TEST : Mutation test systems - not otherwise specified
TEST SYSTEM : Human Lymphocyte
DOSE/DURATION : 100 nmol/L
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
35,99,1975
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Human Lymphocyte
DOSE/DURATION : 5 mg/L
REFERENCE :
CRSBAW Comptes Rendus des Seances de la Societe de Biologie et de Ses
Filiales. (SPPIF, B.P.22, F-41353 Vineuil, France) V.1- 1849-
Volume(issue)/page/year: 179,331,1985
TYPE OF TEST : DNA damage
TEST SYSTEM : Rodent - rat Cells - not otherwise specified
DOSE/DURATION : 5 umol/L
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
51,1078,1991
TYPE OF TEST : Micronucleus test
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 500 ug/kg
REFERENCE :
JTSCDR Journal of Toxicological Sciences. (Japanese Soc. of Toxicological
Sciences, 4th Floor, Gakkai Center Bldg., 4-16, Yayoi 2-chome, Bunkyo-ku,
Tokyo 113, Japan) V.1- 1976- Volume(issue)/page/year: 11(Suppl
1),301,1986
TYPE OF TEST : DNA damage
TEST SYSTEM : Rodent - mouse Leukocyte
DOSE/DURATION : 50 ug/kg
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
40,4225,1980
TYPE OF TEST : DNA damage
TEST SYSTEM : Rodent - mouse Leukocyte
DOSE/DURATION : 2 umol/L
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
44,3360,1984
TYPE OF TEST : DNA damage
TEST SYSTEM : Rodent - mouse Fibroblast
DOSE/DURATION : 400 ug/L
REFERENCE :
MUTAEX Mutagenesis. (Oxford Univ. Press, Pinkhill House, Southfield Road,
Eynsham, Oxford OX8 1JJ, UK) V.1- 1986- Volume(issue)/page/year: 2,179,1987
TYPE OF TEST : DNA inhibition
TEST SYSTEM : Rodent - mouse Cells - not otherwise specified
DOSE/DURATION : 100 ug/L
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
37,2998,1977
TYPE OF TEST : Mutation test systems - not otherwise specified
TEST SYSTEM : Rodent - mouse Cells - not otherwise specified
DOSE/DURATION : 100 ug/L
REFERENCE :
ONCOBS Oncology. (S. Karger AG, Postfach CH-4009 Basel, Switzerland) V.21-
1967- Volume(issue)/page/year: 35,217,1978
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - mouse Lymphocyte
DOSE/DURATION : 500 ug/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 187,141,1987
TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Rodent - mouse Lymphocyte
DOSE/DURATION : 500 ug/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 187,141,1987
TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Rodent - mouse Fibroblast
DOSE/DURATION : 50 ug/L
REFERENCE :
MUTAEX Mutagenesis. (Oxford Univ. Press, Pinkhill House, Southfield Road,
Eynsham, Oxford OX8 1JJ, UK) V.1- 1986- Volume(issue)/page/year: 2,179,1987
TYPE OF TEST : DNA inhibition
TEST SYSTEM : Rodent - hamster Lung
DOSE/DURATION : 4500 nmol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 243,213,1990
TYPE OF TEST : Sister chromatid exchange
TEST SYSTEM : Rodent - hamster Lung
DOSE/DURATION : 1 umol/L
REFERENCE :
CBTOE2 Cell Biology and Toxicology. (Princeton Scientific Pub., Inc., 301
N. Harrison St., CN 5279, Princeton, NJ 08540) V.1- 1984-
Volume(issue)/page/year: 2,485,1986
TYPE OF TEST : Sister chromatid exchange
TEST SYSTEM : Rodent - hamster Ovary
DOSE/DURATION : 10 ug/L
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
43,577,1983
TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Rodent - hamster Ovary
DOSE/DURATION : 40 ug/L
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
43,577,1983
TYPE OF TEST : DNA damage
TEST SYSTEM : Primate - monkey Kidney
DOSE/DURATION : 100 umol/L
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
48,1722,1988
*** REVIEWS ***
TOXICOLOGY REVIEW
EJCAAH European Journal of Cancer. (Oxford, UK) V.1-17(6), 1965-1981. For
publisher information, see EJCODS. Volume(issue)/page/year: 9,477,1973
*** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA ***
NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA :
NOES - National Occupational Exposure Survey (1983)
NOES Hazard Code - X7293
No. of Facilities: 7 (estimated)
No. of Industries: 1
No. of Occupations: 1
No. of Employees: 14 (estimated)
No. of Female Employees: 14 (estimated)
*** STATUS IN U.S. ***
EPA GENETOX PROGRAM 1988, Positive: CHO gene mutation
*** END OF RECORD ***