*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : LM5300000
CHEMICAL NAME : Fluorescein, 4,7-dichloro-2',4',5',7'-tetrabromo-,
dipotassium salt
CAS REGISTRY NUMBER : 6441-77-6
LAST UPDATED : 199504
DATA ITEMS CITED : 14
MOLECULAR FORMULA : C20-H6-Br4-Cl2-O5.2K
MOLECULAR WEIGHT : 795.00
WISWESSER LINE NOTATION : T C666 BO EV IX HHJ DE FE LE MO NE I-& DT56 BVOXJ FG
IG &-KA-
COMPOUND DESCRIPTOR : Mutagen
Reproductive Effector
SYNONYMS/TRADE NAMES :
* C.I. 45405
* C.I. Acid Red 98
* 4,6-Dichloro-2',4',5',7'-tetrabromofluorescein dipotassium salt
* Fluorescein, 2',4',5',7'-tetrabromo-4,7-dichloro-, dipotassium salt
* Phloxin
* Phloxine
* Phloxine K
* 2,4,5,7-Tetrabromo-12,15-dichlorofluorescein, dipotassium salt
* Toyo Acid Phloxine
*** HEALTH HAZARD DATA ***
** ACUTE TOXICITY DATA **
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 2870 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
SKEZAP Shokuhin Eiseigaku Zasshi. Food Hygiene Journal. (Nippon Shokuhin
Eisei Gakkai c/o Shokuhin Eisei Senta, 2-6-1 Jingumae, Shibuya-ku, Tokyo
150, Japan) V.1- 1960- Volume(issue)/page/year: 16,34,1975
** REPRODUCTIVE DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE : 75 gm/kg
SEX/DURATION : female 1-20 day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death,
e.g., stunted fetus)
Reproductive - Effects on Newborn - growth statistics (e.g.%, reduced weight
gain)
REFERENCE :
SKEZAP Shokuhin Eiseigaku Zasshi. Food Hygiene Journal. (Nippon Shokuhin
Eisei Gakkai c/o Shokuhin Eisei Senta, 2-6-1 Jingumae, Shibuya-ku, Tokyo
150, Japan) V.1- 1960- Volume(issue)/page/year: 16,34,1975
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE : 6720 mg/kg
SEX/DURATION : female 28 day(s) pre-mating - 7 day(s) post-birth
TOXIC EFFECTS :
Reproductive - Effects on Newborn - growth statistics (e.g.%, reduced weight
gain)
REFERENCE :
FCTOD7 Food and Chemical Toxicology. (Pergamon Press Inc., Maxwell House,
Fairview Park, Elmsford, NY 10523) V.20- 1982- Volume(issue)/page/year:
31,1013,1993
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE : 6360 mg/kg
SEX/DURATION : female 28 day(s) pre-mating - 4 day(s) post-birth
TOXIC EFFECTS :
Reproductive - Effects on Newborn - behavioral
REFERENCE :
FCTOD7 Food and Chemical Toxicology. (Pergamon Press Inc., Maxwell House,
Fairview Park, Elmsford, NY 10523) V.20- 1982- Volume(issue)/page/year:
31,1013,1993
** MUTATION DATA **
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 15 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 16,165,1972
TYPE OF TEST : DNA damage
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 1 mg/disc
REFERENCE :
FCTXAV Food and Cosmetics Toxicology. (London, UK) V.1-19, 1963-81. For
publisher information, see FCTOD7. Volume(issue)/page/year: 18,215,1980
TYPE OF TEST : Mutation test systems - not otherwise specified
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 100 umol/L
REFERENCE :
SKEZAP Shokuhin Eiseigaku Zasshi. Food Hygiene Journal. (Nippon Shokuhin
Eisei Gakkai c/o Shokuhin Eisei Senta, 2-6-1 Jingumae, Shibuya-ku, Tokyo
150, Japan) V.1- 1960- Volume(issue)/page/year: 12,298,1971
TYPE OF TEST : DNA repair
TEST SYSTEM : Bacteria - Bacillus subtilis
DOSE/DURATION : 2 mg/disc
REFERENCE :
TRENAF Kenkyu Nenpo--Tokyo-toritsu Eisei Kenkyusho. Annual Report of Tokyo
Metropolitan Research Laboratory of Public Health. (Tokyo-toritsu Eisei
Kenkyusho, 24-1, 3-chome, Hyakunin-cho, Shin-juku-ku, Tokyo 160, Japan) V.1-
1949/50- Volume(issue)/page/year: 27,153,1976
TYPE OF TEST : DNA inhibition
TEST SYSTEM : Human Leukocyte
DOSE/DURATION : 500 mg/L
REFERENCE :
NEZAAQ Nippon Eiseigaku Zasshi. Japanese Journal of Hygiene. (Nippon Eisei
Gakkai, c/o Kyoto Daigaku Igakubu, Konoe-cho, Yoshida, Sakyo-ku, Kyoto 606,
Japan) V.1- 1946- Volume(issue)/page/year: 30,574,1975
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Human Leukocyte
DOSE/DURATION : 1 mg/L
REFERENCE :
NEZAAQ Nippon Eiseigaku Zasshi. Japanese Journal of Hygiene. (Nippon Eisei
Gakkai, c/o Kyoto Daigaku Igakubu, Konoe-cho, Yoshida, Sakyo-ku, Kyoto 606,
Japan) V.1- 1946- Volume(issue)/page/year: 30,574,1975
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Human Fibroblast
DOSE/DURATION : 1 mg/L
REFERENCE :
NEZAAQ Nippon Eiseigaku Zasshi. Japanese Journal of Hygiene. (Nippon Eisei
Gakkai, c/o Kyoto Daigaku Igakubu, Konoe-cho, Yoshida, Sakyo-ku, Kyoto 606,
Japan) V.1- 1946- Volume(issue)/page/year: 30,574,1975
*** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA ***
NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA :
NOES - National Occupational Exposure Survey (1983)
NOES Hazard Code - X4118
No. of Facilities: 19 (estimated)
No. of Industries: 1
No. of Occupations: 2
No. of Employees: 400 (estimated)
No. of Female Employees: 340 (estimated)
*** STATUS IN U.S. ***
EPA GENETOX PROGRAM 1988, Positive: B subtilis rec assay
EPA GENETOX PROGRAM 1988, Negative: B subtilis rec assay
*** END OF RECORD ***