*** CHEMICAL IDENTIFICATION ***

RTECS NUMBER            : LM5425000
CHEMICAL NAME           : Fluorescein, disodium salt
CAS REGISTRY NUMBER     : 518-47-8
LAST UPDATED            : 199701
DATA ITEMS CITED        : 19
MOLECULAR FORMULA       : C20-H10-O5.2Na
MOLECULAR WEIGHT        : 376.28
WISWESSER LINE NOTATION : T C666 BO EVJ IR BVQ& MQ &-NA- 2
COMPOUND DESCRIPTOR     : Tumorigen
                          Drug
                          Mutagen
                          Reproductive Effector
                          Human
SYNONYMS/TRADE NAMES :
   * Aizen uranine
   * Calcocid uranine B4315
   * 9-o-Carboxyphenyl-6-hydroxy-3-isoxanthone, disodium salt
   * Certiqual fluoresceine
   * C.I. 766
   * C.I. Acid Yellow 73
   * C.I. 45350 disodium salt
   * C.I. 45350 sodium salt
   * D&C Yellow No. 8
   * Disodium 6-hydroxy-3-oxo-9-xanthene-o-benzoate
   * Fluorescein sodium
   * Fluorescein sodium B.P
   * Fluorescein, soluble
   * Fluor-I-strip A.T.
   * Ful-Glo
   * Funduscein
   * Furanium
   * Hidacid uranine
   * NCI-C54706
   * Resorcinol phthalein sodium
   * Sodium fluorescein
   * Sodium fluoresceinate
   * Sodium salt of hydroxy-o-carboxy-phenyl-fluorone
   * Soluble fluorescein
   * Soluble fluoresceine
   * Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 3',6'-dihydroxy-,  
   disodium salt
   * Uranin
   * Uranine
   * Uranine A Extra
   * Uranine O
   * Uranine SS
   * Uranine USP XII
   * Uranine WSS
   * Uranine Yellow
   * 11824 Yellow
   * 12417 Yellow
 
                       *** HEALTH HAZARD DATA ***
 
                        ** ACUTE TOXICITY DATA **
 
TYPE OF TEST            : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Human - man
DOSE/DURATION           : 14 mg/kg/10M-I
TOXIC EFFECTS :
   Cardiac - arrhythmias (including changes in conduction)
REFERENCE :
   AACRAT Anesthesia and Analgesia (New York).  (Elsevier Science Pub. Co.,
   Inc., 52 Vanderbilt Ave., New York, NY 10017)  V.36-    1957-
   Volume(issue)/page/year: 66,283,1987
 
TYPE OF TEST            : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Human - man
DOSE/DURATION           : 7142 ug/kg
TOXIC EFFECTS :
   Sense Organs and Special Senses (Eye) - hemorrhage
   Gastrointestinal - nausea or vomiting
REFERENCE :
   AJOPAA American Journal of Ophthalmology.  (Ophthalmic Pub. Co., 435 N.
   Michigan Ave., Suite 1415, Chicago, IL 60611)  Series 3: V.1-    1918-
   Volume(issue)/page/year: 103,111,1987
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Oral
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 6721 mg/kg
TOXIC EFFECTS :
   Behavioral - changes in motor activity (specific assay)
   Behavioral - ataxia
   Lungs, Thorax, or Respiration - dyspnea
REFERENCE :
   JOPRAJ Journal of Periodontology.  (American Academy of Periodontology, 211
   E. Chicago Ave., Chicago, IL 60611)  V.1-    1930- Volume(issue)/page/year:
   48,228,1977
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 1 gm/kg
TOXIC EFFECTS :
   Blood - other changes
REFERENCE :
   TXAPA9 Toxicology and Applied Pharmacology.  (Academic Press, Inc., 1 E.
   First St., Duluth, MN 55802) V.1-    1959-  Volume(issue)/page/year:
   29,97,1974
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Oral
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 4738 mg/kg
TOXIC EFFECTS :
   Behavioral - changes in motor activity (specific assay)
   Behavioral - ataxia
   Lungs, Thorax, or Respiration - dyspnea
REFERENCE :
   JOPRAJ Journal of Periodontology.  (American Academy of Periodontology, 211
   E. Chicago Ave., Chicago, IL 60611)  V.1-    1930- Volume(issue)/page/year:
   48,228,1977
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 1 gm/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   TXAPA9 Toxicology and Applied Pharmacology.  (Academic Press, Inc., 1 E.
   First St., Duluth, MN 55802) V.1-    1959-  Volume(issue)/page/year:
   29,97,1974
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Mammal - dog
DOSE/DURATION           : 1 gm/kg
TOXIC EFFECTS :
   Gastrointestinal - changes in structure or function of salivary glands
REFERENCE :
   TXAPA9 Toxicology and Applied Pharmacology.  (Academic Press, Inc., 1 E.
   First St., Duluth, MN 55802) V.1-    1959-  Volume(issue)/page/year:
   29,97,1974
 
TYPE OF TEST            : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE       : Intraperitoneal
SPECIES OBSERVED        : Rodent - guinea pig
DOSE/DURATION           : 1 gm/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   CWLTM* Chemical Warfare Laboratories Technical Memorandum.  (U.S. Army
   Chemical Center, Edgewood Arsenal, MD 21010)  Volume(issue)/page/year:
   #47-6,1959
 
                 ** OTHER MULTIPLE DOSE TOXICITY DATA **
 
TYPE OF TEST            : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 4 gm/kg/28D-I
TOXIC EFFECTS :
   Blood - pigmented or nucleated red blood cells
REFERENCE :
   TXAPA9 Toxicology and Applied Pharmacology.  (Academic Press, Inc., 1 E.
   First St., Duluth, MN 55802) V.1-    1959-  Volume(issue)/page/year:
   29,97,1974
 
TYPE OF TEST            : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Mammal - dog
DOSE/DURATION           : 4 gm/kg/28D-I
TOXIC EFFECTS :
   Gastrointestinal - nausea or vomiting
   Biochemical - Enzyme inhibition, induction, or change in blood or tissue
   levels - transaminases
   Related to Chronic Data - death
REFERENCE :
   TXAPA9 Toxicology and Applied Pharmacology.  (Academic Press, Inc., 1 E.
   First St., Duluth, MN 55802) V.1-    1959-  Volume(issue)/page/year:
   29,97,1974
 
                          ** TUMORIGENIC DATA **
 
TYPE OF TEST            : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE       : Subcutaneous
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 19 gm/kg/79W-I
TOXIC EFFECTS :
   Tumorigenic - equivocal tumorigenic agent by RTECS criteria
   Tumorigenic - tumors at site of application
REFERENCE :
   GANNA2 Gann.  Japanese Journal of Cancer Research.  (Tokyo, Japan)  V.1-75,
   1907-84.  For publisher information, see JJCREP.  Volume(issue)/page/year:
   45,446,1954
 
TYPE OF TEST            : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE       : Subcutaneous
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 16 gm/kg/1Y-I
TOXIC EFFECTS :
   Tumorigenic - equivocal tumorigenic agent by RTECS criteria
   Skin and Appendages - tumors
   Tumorigenic - tumors at site of application
REFERENCE :
   GANNA2 Gann.  Japanese Journal of Cancer Research.  (Tokyo, Japan)  V.1-75,
   1907-84.  For publisher information, see JJCREP.  Volume(issue)/page/year:
   47,51,1956
 
                          ** REPRODUCTIVE DATA **
 
TYPE OF TEST            : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Rodent - rabbit
DOSE                    : 933 mg/kg
SEX/DURATION            : female 5-8 day(s) after conception
TOXIC EFFECTS :
   Reproductive - Maternal Effects - postpartum
   Reproductive - Effects on Newborn - viability index (e.g., # alive at day 4
   per # born alive)
REFERENCE :
   AJOPAA American Journal of Ophthalmology.  (Ophthalmic Pub. Co., 435 N.
   Michigan Ave., Suite 1415, Chicago, IL 60611)  Series 3: V.1-    1918-
   Volume(issue)/page/year: 84,847,1977
 
                            ** MUTATION DATA **
 
TYPE OF TEST            : DNA adduct
TEST SYSTEM             : Bacteria - Escherichia coli
DOSE/DURATION           : 15 umol/L
REFERENCE :
   MUREAV Mutation Research.  (Elsevier Science Pub. B.V., POB 211, 1000 AE
   Amsterdam, Netherlands) V.1-    1964-  Volume(issue)/page/year: 89,95,1981
 
TYPE OF TEST            : Mutation in mammalian somatic cells
TEST SYSTEM             : Rodent - mouse Lymphocyte
DOSE/DURATION           : 200 mg/L
REFERENCE :
   SCIEAS Science.  (American Assoc. for the Advancement of Science, 1333 H
   St., NW, Washington, DC 20005)  V.1-    1895-  Volume(issue)/page/year:
   236,933,1987
 
TYPE OF TEST            : Sister chromatid exchange
TEST SYSTEM             : Rodent - hamster Ovary
DOSE/DURATION           : 50100 ug/L
REFERENCE :
   SCIEAS Science.  (American Assoc. for the Advancement of Science, 1333 H
   St., NW, Washington, DC 20005)  V.1-    1895-  Volume(issue)/page/year:
   236,933,1987

         *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA ***

NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA :
   NOHS - National Occupational Hazard Survey (1974)
     NOHS Hazard Code - M1436
       No. of Facilities: 2278 (estimated)
       No. of Industries: 18
       No. of Occupations: 21
       No. of Employees: 14785 (estimated)
   
   NOES - National Occupational Exposure Survey (1983)
     NOES Hazard Code - M1436
       No. of Facilities: 5145 (estimated)
       No. of Industries: 76
       No. of Occupations: 69
       No. of Employees: 94491 (estimated)
       No. of Female Employees: 21830 (estimated)

                          *** STATUS IN U.S. ***
 
   EPA TSCA Section 8(b) CHEMICAL INVENTORY

 
                            *** END OF RECORD ***