*** CHEMICAL IDENTIFICATION *** RTECS NUMBER : LM5425000 CHEMICAL NAME : Fluorescein, disodium salt CAS REGISTRY NUMBER : 518-47-8 LAST UPDATED : 199701 DATA ITEMS CITED : 19 MOLECULAR FORMULA : C20-H10-O5.2Na MOLECULAR WEIGHT : 376.28 WISWESSER LINE NOTATION : T C666 BO EVJ IR BVQ& MQ &-NA- 2 COMPOUND DESCRIPTOR : Tumorigen Drug Mutagen Reproductive Effector Human SYNONYMS/TRADE NAMES : * Aizen uranine * Calcocid uranine B4315 * 9-o-Carboxyphenyl-6-hydroxy-3-isoxanthone, disodium salt * Certiqual fluoresceine * C.I. 766 * C.I. Acid Yellow 73 * C.I. 45350 disodium salt * C.I. 45350 sodium salt * D&C Yellow No. 8 * Disodium 6-hydroxy-3-oxo-9-xanthene-o-benzoate * Fluorescein sodium * Fluorescein sodium B.P * Fluorescein, soluble * Fluor-I-strip A.T. * Ful-Glo * Funduscein * Furanium * Hidacid uranine * NCI-C54706 * Resorcinol phthalein sodium * Sodium fluorescein * Sodium fluoresceinate * Sodium salt of hydroxy-o-carboxy-phenyl-fluorone * Soluble fluorescein * Soluble fluoresceine * Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 3',6'-dihydroxy-, disodium salt * Uranin * Uranine * Uranine A Extra * Uranine O * Uranine SS * Uranine USP XII * Uranine WSS * Uranine Yellow * 11824 Yellow * 12417 Yellow *** HEALTH HAZARD DATA *** ** ACUTE TOXICITY DATA ** TYPE OF TEST : TDLo - Lowest published toxic dose ROUTE OF EXPOSURE : Intravenous SPECIES OBSERVED : Human - man DOSE/DURATION : 14 mg/kg/10M-I TOXIC EFFECTS : Cardiac - arrhythmias (including changes in conduction) REFERENCE : AACRAT Anesthesia and Analgesia (New York). (Elsevier Science Pub. Co., Inc., 52 Vanderbilt Ave., New York, NY 10017) V.36- 1957- Volume(issue)/page/year: 66,283,1987 TYPE OF TEST : TDLo - Lowest published toxic dose ROUTE OF EXPOSURE : Intravenous SPECIES OBSERVED : Human - man DOSE/DURATION : 7142 ug/kg TOXIC EFFECTS : Sense Organs and Special Senses (Eye) - hemorrhage Gastrointestinal - nausea or vomiting REFERENCE : AJOPAA American Journal of Ophthalmology. (Ophthalmic Pub. Co., 435 N. Michigan Ave., Suite 1415, Chicago, IL 60611) Series 3: V.1- 1918- Volume(issue)/page/year: 103,111,1987 TYPE OF TEST : LD50 - Lethal dose, 50 percent kill ROUTE OF EXPOSURE : Oral SPECIES OBSERVED : Rodent - rat DOSE/DURATION : 6721 mg/kg TOXIC EFFECTS : Behavioral - changes in motor activity (specific assay) Behavioral - ataxia Lungs, Thorax, or Respiration - dyspnea REFERENCE : JOPRAJ Journal of Periodontology. (American Academy of Periodontology, 211 E. Chicago Ave., Chicago, IL 60611) V.1- 1930- Volume(issue)/page/year: 48,228,1977 TYPE OF TEST : LD50 - Lethal dose, 50 percent kill ROUTE OF EXPOSURE : Intravenous SPECIES OBSERVED : Rodent - rat DOSE/DURATION : 1 gm/kg TOXIC EFFECTS : Blood - other changes REFERENCE : TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year: 29,97,1974 TYPE OF TEST : LD50 - Lethal dose, 50 percent kill ROUTE OF EXPOSURE : Oral SPECIES OBSERVED : Rodent - mouse DOSE/DURATION : 4738 mg/kg TOXIC EFFECTS : Behavioral - changes in motor activity (specific assay) Behavioral - ataxia Lungs, Thorax, or Respiration - dyspnea REFERENCE : JOPRAJ Journal of Periodontology. (American Academy of Periodontology, 211 E. Chicago Ave., Chicago, IL 60611) V.1- 1930- Volume(issue)/page/year: 48,228,1977 TYPE OF TEST : LD50 - Lethal dose, 50 percent kill ROUTE OF EXPOSURE : Intravenous SPECIES OBSERVED : Rodent - mouse DOSE/DURATION : 1 gm/kg TOXIC EFFECTS : Details of toxic effects not reported other than lethal dose value REFERENCE : TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year: 29,97,1974 TYPE OF TEST : LD50 - Lethal dose, 50 percent kill ROUTE OF EXPOSURE : Intravenous SPECIES OBSERVED : Mammal - dog DOSE/DURATION : 1 gm/kg TOXIC EFFECTS : Gastrointestinal - changes in structure or function of salivary glands REFERENCE : TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year: 29,97,1974 TYPE OF TEST : LDLo - Lowest published lethal dose ROUTE OF EXPOSURE : Intraperitoneal SPECIES OBSERVED : Rodent - guinea pig DOSE/DURATION : 1 gm/kg TOXIC EFFECTS : Details of toxic effects not reported other than lethal dose value REFERENCE : CWLTM* Chemical Warfare Laboratories Technical Memorandum. (U.S. Army Chemical Center, Edgewood Arsenal, MD 21010) Volume(issue)/page/year: #47-6,1959 ** OTHER MULTIPLE DOSE TOXICITY DATA ** TYPE OF TEST : TDLo - Lowest published toxic dose ROUTE OF EXPOSURE : Intravenous SPECIES OBSERVED : Rodent - rat DOSE/DURATION : 4 gm/kg/28D-I TOXIC EFFECTS : Blood - pigmented or nucleated red blood cells REFERENCE : TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year: 29,97,1974 TYPE OF TEST : TDLo - Lowest published toxic dose ROUTE OF EXPOSURE : Intravenous SPECIES OBSERVED : Mammal - dog DOSE/DURATION : 4 gm/kg/28D-I TOXIC EFFECTS : Gastrointestinal - nausea or vomiting Biochemical - Enzyme inhibition, induction, or change in blood or tissue levels - transaminases Related to Chronic Data - death REFERENCE : TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year: 29,97,1974 ** TUMORIGENIC DATA ** TYPE OF TEST : TDLo - Lowest published toxic dose ROUTE OF EXPOSURE : Subcutaneous SPECIES OBSERVED : Rodent - rat DOSE/DURATION : 19 gm/kg/79W-I TOXIC EFFECTS : Tumorigenic - equivocal tumorigenic agent by RTECS criteria Tumorigenic - tumors at site of application REFERENCE : GANNA2 Gann. Japanese Journal of Cancer Research. (Tokyo, Japan) V.1-75, 1907-84. For publisher information, see JJCREP. Volume(issue)/page/year: 45,446,1954 TYPE OF TEST : TD - Toxic dose (other than lowest) ROUTE OF EXPOSURE : Subcutaneous SPECIES OBSERVED : Rodent - rat DOSE/DURATION : 16 gm/kg/1Y-I TOXIC EFFECTS : Tumorigenic - equivocal tumorigenic agent by RTECS criteria Skin and Appendages - tumors Tumorigenic - tumors at site of application REFERENCE : GANNA2 Gann. Japanese Journal of Cancer Research. (Tokyo, Japan) V.1-75, 1907-84. For publisher information, see JJCREP. Volume(issue)/page/year: 47,51,1956 ** REPRODUCTIVE DATA ** TYPE OF TEST : TDLo - Lowest published toxic dose ROUTE OF EXPOSURE : Intravenous SPECIES OBSERVED : Rodent - rabbit DOSE : 933 mg/kg SEX/DURATION : female 5-8 day(s) after conception TOXIC EFFECTS : Reproductive - Maternal Effects - postpartum Reproductive - Effects on Newborn - viability index (e.g., # alive at day 4 per # born alive) REFERENCE : AJOPAA American Journal of Ophthalmology. (Ophthalmic Pub. Co., 435 N. Michigan Ave., Suite 1415, Chicago, IL 60611) Series 3: V.1- 1918- Volume(issue)/page/year: 84,847,1977 ** MUTATION DATA ** TYPE OF TEST : DNA adduct TEST SYSTEM : Bacteria - Escherichia coli DOSE/DURATION : 15 umol/L REFERENCE : MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 89,95,1981 TYPE OF TEST : Mutation in mammalian somatic cells TEST SYSTEM : Rodent - mouse Lymphocyte DOSE/DURATION : 200 mg/L REFERENCE : SCIEAS Science. (American Assoc. for the Advancement of Science, 1333 H St., NW, Washington, DC 20005) V.1- 1895- Volume(issue)/page/year: 236,933,1987 TYPE OF TEST : Sister chromatid exchange TEST SYSTEM : Rodent - hamster Ovary DOSE/DURATION : 50100 ug/L REFERENCE : SCIEAS Science. (American Assoc. for the Advancement of Science, 1333 H St., NW, Washington, DC 20005) V.1- 1895- Volume(issue)/page/year: 236,933,1987 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - M1436 No. of Facilities: 2278 (estimated) No. of Industries: 18 No. of Occupations: 21 No. of Employees: 14785 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - M1436 No. of Facilities: 5145 (estimated) No. of Industries: 76 No. of Occupations: 69 No. of Employees: 94491 (estimated) No. of Female Employees: 21830 (estimated) *** STATUS IN U.S. *** EPA TSCA Section 8(b) CHEMICAL INVENTORY *** END OF RECORD ***