*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : LM5950000
CHEMICAL NAME : Fluorescein, 2',4',5',7'-tetraiodo-, disodium salt
CAS REGISTRY NUMBER : 16423-68-0
LAST UPDATED : 199710
DATA ITEMS CITED : 31
MOLECULAR FORMULA : C20-H6-I4-O5.2Na
MOLECULAR WEIGHT : 879.84
WISWESSER LINE NOTATION : T C666 BO EVJ DI FI IR BVQ& LI MQ NI &-NA- 2
COMPOUND DESCRIPTOR : Tumorigen
Mutagen
Reproductive Effector
SYNONYMS/TRADE NAMES :
* Aizen erythrosine
* Calcocid erythrosine N
* Canacert erythrosine BS
* 9-(o-Carboxyphenyl)-6-hydroxy-2,4,5,7-tetraiodo-3H-xanthene-3-one
disodium salt monohydrate
* Cerven kysela 51
* Cerven potravinarska 14
* C.I. 773
* C.I. 45430
* C.I. Acid Red 51
* C.I. Food Red 14
* Cilefa Pink B
* D&C Red No. 3
* Dolkwal erythrosine
* Dye FD and C Red No. 3
* E 127
* EBS
* Edicol Supra Erythrosine A
* Erythrosin
* Erythrosin B
* Erythrosine
* Erythrosine B
* Erythrosine 3B
* Erythrosine B (biological stain)
* Erythrosine B-FO (biological stain)
* Erythrosine bluish
* Erythrosine bluish (biological stain)
* Erythrosine BS
* Erythrosine Extra Bluish
* Erythrosine Extra Conc. A Export
* Erythrosine Extra Pure A
* Erythrosine (indicator)
* Erythrosine K-FO (biological stain)
* Erythrosine Lake
* Erythrosine sodium
* Erythrosine TB
* Erythrosine TB Extra
* FD and C Red No. 3
* Food Red 14
* Hexacert Red No. 3
* Hexacol erythrosine BS
* LB-Rot 1
* Maple erythrosine
* New Pink Bluish Geigy
* 1427 Red
* 1671 Red
* Schultz No. 887
* Tetraiodofluorescein sodium salt
* Usacert Red No. 3
*** HEALTH HAZARD DATA ***
** ACUTE TOXICITY DATA **
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 1840 mg/kg
TOXIC EFFECTS :
Behavioral - convulsions or effect on seizure threshold
REFERENCE :
SCPHA4 Scientia Pharmaceutica. (Oesterreichische
Apotheker-Verlagsgesellschaft MBH, Spitalgasse 31, 1094 Vienna 9, Austria)
V.1- 1930- Volume(issue)/page/year: 47,39,1979
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 300 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
FAONAU FAO Nutrition Meetings Report Series. (Rome, Italy) No.?-57,
1948-77. Discontinued. Volume(issue)/page/year: 38B,50,1966
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 200 mg/kg
TOXIC EFFECTS :
Behavioral - convulsions or effect on seizure threshold
REFERENCE :
SCPHA4 Scientia Pharmaceutica. (Oesterreichische
Apotheker-Verlagsgesellschaft MBH, Spitalgasse 31, 1094 Vienna 9, Austria)
V.1- 1930- Volume(issue)/page/year: 47,39,1979
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Unreported
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 1895 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
NAIZAM Nara Igaku Zasshi. Journal of the Nara Medical Association. (Nara
Igakkai, c/o Narakenritsu Ika Daigaku, Shijo-cho, Kashihara, Nara-ken,
Japan) V.1- 1950- Volume(issue)/page/year: 30,179,1979
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 1264 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
EAPHA6 Eastern Pharmacist. (507, Ashok Bhawan, 93, Neru Place, New Delhi
110 019, India) V.1- 1958- Volume(issue)/page/year: 24,125,1981
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 370 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
APPHAX Acta Poloniae Pharmaceutica. For English translation, see APPFAR.
(Ars Polona, POB 1001, 00-680 Warsaw 1, Poland) V.1- 1937-
Volume(issue)/page/year: 9,127,1952
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - rabbit
DOSE/DURATION : 200 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
FAONAU FAO Nutrition Meetings Report Series. (Rome, Italy) No.?-57,
1948-77. Discontinued. Volume(issue)/page/year: 38B,50,1966
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - gerbil
DOSE/DURATION : 1930 mg/kg
TOXIC EFFECTS :
Behavioral - convulsions or effect on seizure threshold
REFERENCE :
SCPHA4 Scientia Pharmaceutica. (Oesterreichische
Apotheker-Verlagsgesellschaft MBH, Spitalgasse 31, 1094 Vienna 9, Austria)
V.1- 1930- Volume(issue)/page/year: 47,39,1979
** OTHER MULTIPLE DOSE TOXICITY DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 90 gm/kg/90D-C
TOXIC EFFECTS :
Gastrointestinal - other changes
Endocrine - changes in thyroid weight
REFERENCE :
FCTXAV Food and Cosmetics Toxicology. (London, UK) V.1-19, 1963-81. For
publisher information, see FCTOD7. Volume(issue)/page/year: 14,525,1976
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Mammal - pig
DOSE/DURATION : 147 gm/kg/14W-I
TOXIC EFFECTS :
Endocrine - changes in thyroid weight
Blood - changes in leukocyte (WBC) count
REFERENCE :
FCTXAV Food and Cosmetics Toxicology. (London, UK) V.1-19, 1963-81. For
publisher information, see FCTOD7. Volume(issue)/page/year: 14,533,1976
** TUMORIGENIC DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 1798 gm/kg/2Y-C
TOXIC EFFECTS :
Tumorigenic - neoplastic by RTECS criteria
Endocrine - thyroid tumors
REFERENCE :
FCTOD7 Food and Chemical Toxicology. (Pergamon Press Inc., Maxwell House,
Fairview Park, Elmsford, NY 10523) V.20- 1982- Volume(issue)/page/year:
25,723,1987
** REPRODUCTIVE DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE : 482 gm/kg
SEX/DURATION : multigeneration
TOXIC EFFECTS :
Reproductive - Effects on Newborn - growth statistics (e.g.%, reduced weight
gain)
REFERENCE :
FCTOD7 Food and Chemical Toxicology. (Pergamon Press Inc., Maxwell House,
Fairview Park, Elmsford, NY 10523) V.20- 1982- Volume(issue)/page/year:
28,813,1990
** MUTATION DATA **
TYPE OF TEST : Mutation test systems - not otherwise specified
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 100 umol/L
REFERENCE :
SKEZAP Shokuhin Eiseigaku Zasshi. Food Hygiene Journal. (Nippon Shokuhin
Eisei Gakkai c/o Shokuhin Eisei Senta, 2-6-1 Jingumae, Shibuya-ku, Tokyo
150, Japan) V.1- 1960- Volume(issue)/page/year: 12,298,1971
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Bacillus subtilis
DOSE/DURATION : 100 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 206,171,1988
TYPE OF TEST : DNA repair
TEST SYSTEM : Bacteria - Bacillus subtilis
DOSE/DURATION : 2 mg/disc
REFERENCE :
TRENAF Kenkyu Nenpo--Tokyo-toritsu Eisei Kenkyusho. Annual Report of Tokyo
Metropolitan Research Laboratory of Public Health. (Tokyo-toritsu Eisei
Kenkyusho, 24-1, 3-chome, Hyakunin-cho, Shin-juku-ku, Tokyo 160, Japan) V.1-
1949/50- Volume(issue)/page/year: 27,153,1976
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Yeast - Saccharomyces cerevisiae
DOSE/DURATION : 100 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 138,153,1984
TYPE OF TEST : Gene conversion and mitotic recombination
TEST SYSTEM : Yeast - Saccharomyces cerevisiae
DOSE/DURATION : 100 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 138,153,1984
TYPE OF TEST : DNA inhibition
TEST SYSTEM : Human Leukocyte
DOSE/DURATION : 500 mg/L
REFERENCE :
NEZAAQ Nippon Eiseigaku Zasshi. Japanese Journal of Hygiene. (Nippon Eisei
Gakkai, c/o Kyoto Daigaku Igakubu, Konoe-cho, Yoshida, Sakyo-ku, Kyoto 606,
Japan) V.1- 1946- Volume(issue)/page/year: 30,574,1975
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Rodent - mouse Lymphocyte
DOSE/DURATION : 120 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 189,223,1987
TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Rodent - mouse Lymphocyte
DOSE/DURATION : 120 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 189,223,1987
TYPE OF TEST : Micronucleus test
TEST SYSTEM : Rodent - hamster Lung
DOSE/DURATION : 300 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 205,415,1988
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Fibroblast
DOSE/DURATION : 600 mg/L
REFERENCE :
FCTOD7 Food and Chemical Toxicology. (Pergamon Press Inc., Maxwell House,
Fairview Park, Elmsford, NY 10523) V.20- 1982- Volume(issue)/page/year:
22,623,1984
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Lung
DOSE/DURATION : 1100 mg/L
REFERENCE :
GMCRDC Gann Monograph on Cancer Research. (Plenum Pub. Corp., 233 Spring
St., New York, NY 10013) No. 11- 1971- Volume(issue)/page/year:
27,95,1981
*** U.S. STANDARDS AND REGULATIONS ***
EPA FIFRA 1988 PESTICIDE SUBJECT TO REGISTRATION OR RE-REGISTRATION
FEREAC Federal Register. (U.S. Government Printing Office, Supt. of
Documents, Washington, DC 20402) V.1- 1936- Volume(issue)/page/year:
54,7740,1989
*** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA ***
NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA :
NOHS - National Occupational Hazard Survey (1974)
NOHS Hazard Code - M4011
No. of Facilities: 904 (estimated)
No. of Industries: 5
No. of Occupations: 11
No. of Employees: 8221 (estimated)
NOES - National Occupational Exposure Survey (1983)
NOES Hazard Code - M4011
No. of Facilities: 2991 (estimated)
No. of Industries: 17
No. of Occupations: 39
No. of Employees: 67011 (estimated)
No. of Female Employees: 25006 (estimated)
*** STATUS IN U.S. ***
EPA GENETOX PROGRAM 1988, Negative: B subtilis rec assay; Histidine
reversion-Ames test
EPA GENETOX PROGRAM 1988, Negative: S cerevisiae gene conversion
EPA GENETOX PROGRAM 1988, Inconclusive: B subtilis rec assay
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JUNE 1998
*** END OF RECORD ***