*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : LV2500000
CHEMICAL NAME : Furo(3',4':6,7)naphtho(2,3-d)-1,3-dioxol-6(5ah)-one,
5,8,8a,9-tetrahydro-9-hydroxy-5-
(3,4,5-trimethoxyphenyl)-
CAS REGISTRY NUMBER : 518-28-5
LAST UPDATED : 199806
DATA ITEMS CITED : 36
MOLECULAR FORMULA : C22-H22-O8
MOLECULAR WEIGHT : 414.44
WISWESSER LINE NOTATION : T E5 C665 FVO NO PO OHTT&&J DR CO1 DO1 EO1& JQ
COMPOUND DESCRIPTOR : Drug
Mutagen
Reproductive Effector
Natural Product
SYNONYMS/TRADE NAMES :
* Condylox
* NSC 24818
* Podofilox
* Podophyllinic acid lactone
* Podophyllotoxin
*** HEALTH HAZARD DATA ***
** ACUTE TOXICITY DATA **
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Administration onto the skin
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 500 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
PIXXD2 PCT (Patent Cooperation Treaty) International Application. (U.S.
Patent and Trademark Office, Foreign Patents, Washington, DC 20231)
Volume(issue)/page/year: #86-04062
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 15 mg/kg
TOXIC EFFECTS :
Behavioral - somnolence (general depressed activity)
Lungs, Thorax, or Respiration - dyspnea
Gastrointestinal - nausea or vomiting
REFERENCE :
FEPRA7 Federation Proceedings, Federation of American Societies for
Experimental Biology. (Bethesda, MD) V.1-46, 1942-87.
Volume(issue)/page/year: 7,249,1948
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 8 mg/kg
TOXIC EFFECTS :
Behavioral - somnolence (general depressed activity)
Lungs, Thorax, or Respiration - dyspnea
Lungs, Thorax, or Respiration - respiratory depression
REFERENCE :
PSEBAA Proceedings of the Society for Experimental Biology and Medicine.
(Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1903/04-
Volume(issue)/page/year: 77,269,1951
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 8700 ug/kg
TOXIC EFFECTS :
Behavioral - somnolence (general depressed activity)
Lungs, Thorax, or Respiration - dyspnea
Gastrointestinal - nausea or vomiting
REFERENCE :
FEPRA7 Federation Proceedings, Federation of American Societies for
Experimental Biology. (Bethesda, MD) V.1-46, 1942-87.
Volume(issue)/page/year: 7,249,1948
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intramuscular
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 3 mg/kg
TOXIC EFFECTS :
Behavioral - somnolence (general depressed activity)
Lungs, Thorax, or Respiration - dyspnea
Gastrointestinal - nausea or vomiting
REFERENCE :
FEPRA7 Federation Proceedings, Federation of American Societies for
Experimental Biology. (Bethesda, MD) V.1-46, 1942-87.
Volume(issue)/page/year: 7,249,1948
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 100 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
PIXXD2 PCT (Patent Cooperation Treaty) International Application. (U.S.
Patent and Trademark Office, Foreign Patents, Washington, DC 20231)
Volume(issue)/page/year: #86-04062
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 30 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
ARZNAD Arzneimittel-Forschung. Drug Research. (Editio Cantor Verlag,
Postfach 1255, W-7960 Aulendorf, Fed. Rep. Ger.) V.1- 1951-
Volume(issue)/page/year: 11,327,1961
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 24600 ug/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
TOXIA6 Toxicon. (Pergamon Press Ltd., Headington Hill Hall, Oxford OX3 OBW,
UK) V.1- 1962- Volume(issue)/page/year: 8,85,1970
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 56 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
CSLNX* U.S. Army Armament Research & Development Command, Chemical Systems
Laboratory, NIOSH Exchange Chemicals. (Aberdeen Proving Ground, MD 21010)
Volume(issue)/page/year: NX#01679
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Mammal - dog
DOSE/DURATION : 1 mg/kg
TOXIC EFFECTS :
Behavioral - convulsions or effect on seizure threshold
Gastrointestinal - nausea or vomiting
Nutritional and Gross Metabolic - weight loss or decreased weight gain
REFERENCE :
AEXPBL Archiv fuer Experimentelle Pathologie und Pharmakologie. (Leipzig,
Ger. Dem. Rep.) V.1-109, 1873-1925. For publisher information, see NSAPCC.
Volume(issue)/page/year: 28,32,1891
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Mammal - cat
DOSE/DURATION : 300 ug/kg
TOXIC EFFECTS :
Behavioral - convulsions or effect on seizure threshold
Gastrointestinal - nausea or vomiting
Nutritional and Gross Metabolic - weight loss or decreased weight gain
REFERENCE :
AEXPBL Archiv fuer Experimentelle Pathologie und Pharmakologie. (Leipzig,
Ger. Dem. Rep.) V.1-109, 1873-1925. For publisher information, see NSAPCC.
Volume(issue)/page/year: 28,32,1891
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Mammal - cat
DOSE/DURATION : 1700 ug/kg
TOXIC EFFECTS :
Behavioral - somnolence (general depressed activity)
Lungs, Thorax, or Respiration - dyspnea
Gastrointestinal - nausea or vomiting
REFERENCE :
FEPRA7 Federation Proceedings, Federation of American Societies for
Experimental Biology. (Bethesda, MD) V.1-46, 1942-87.
Volume(issue)/page/year: 7,249,1948
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intramuscular
SPECIES OBSERVED : Mammal - cat
DOSE/DURATION : 4 mg/kg
TOXIC EFFECTS :
Behavioral - somnolence (general depressed activity)
Lungs, Thorax, or Respiration - dyspnea
Skin and Appendages - cutaneous sensitization, experimental (after topical
exposure)
REFERENCE :
FEPRA7 Federation Proceedings, Federation of American Societies for
Experimental Biology. (Bethesda, MD) V.1-46, 1942-87.
Volume(issue)/page/year: 7,249,1948
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Administration onto the skin
SPECIES OBSERVED : Rodent - rabbit
DOSE/DURATION : 200 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
PIXXD2 PCT (Patent Cooperation Treaty) International Application. (U.S.
Patent and Trademark Office, Foreign Patents, Washington, DC 20231)
Volume(issue)/page/year: #86-04062
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - rabbit
DOSE/DURATION : 5 mg/kg
TOXIC EFFECTS :
Behavioral - somnolence (general depressed activity)
Lungs, Thorax, or Respiration - dyspnea
Skin and Appendages - cutaneous sensitization, experimental (after topical
exposure)
REFERENCE :
FEPRA7 Federation Proceedings, Federation of American Societies for
Experimental Biology. (Bethesda, MD) V.1-46, 1942-87.
Volume(issue)/page/year: 7,249,1948
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Bird - pigeon
DOSE/DURATION : 10 mg/kg
TOXIC EFFECTS :
Behavioral - muscle contraction or spasticity
Gastrointestinal - hypermotility, diarrhea
REFERENCE :
AEXPBL Archiv fuer Experimentelle Pathologie und Pharmakologie. (Leipzig,
Ger. Dem. Rep.) V.1-109, 1873-1925. For publisher information, see NSAPCC.
Volume(issue)/page/year: 28,32,1891
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Bird - chicken
DOSE/DURATION : 6250 ug/kg
TOXIC EFFECTS :
Behavioral - muscle contraction or spasticity
Gastrointestinal - hypermotility, diarrhea
REFERENCE :
AEXPBL Archiv fuer Experimentelle Pathologie und Pharmakologie. (Leipzig,
Ger. Dem. Rep.) V.1-109, 1873-1925. For publisher information, see NSAPCC.
Volume(issue)/page/year: 28,32,1891
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Parenteral
SPECIES OBSERVED : Amphibian - frog
DOSE/DURATION : 152 mg/kg
TOXIC EFFECTS :
Behavioral - stiffness
REFERENCE :
AEXPBL Archiv fuer Experimentelle Pathologie und Pharmakologie. (Leipzig,
Ger. Dem. Rep.) V.1-109, 1873-1925. For publisher information, see NSAPCC.
Volume(issue)/page/year: 28,32,1891
** OTHER MULTIPLE DOSE TOXICITY DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 11690 ug/kg/7D-I
TOXIC EFFECTS :
Gastrointestinal - other changes
Liver - other changes
REFERENCE :
TOXID9 Toxicologist. (Soc. of Toxicology, Inc., 475 Wolf Ledge Parkway,
Akron, OH 44311) V.1- 1981- Volume(issue)/page/year: 15,291,1995
** REPRODUCTIVE DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE : 500 mg/kg
SEX/DURATION : female 1-5 day(s) after conception
TOXIC EFFECTS :
Reproductive - Fertility - female fertility index (e.g. # females pregnant
per # sperm positive females; # females pregnant per # females mated)
REFERENCE :
JRPFA4 Journal of Reproduction and Fertility. (Biochemical Soc. Book Depot,
POB 32, Commerce Way, Colchester, Essex CO2 8HP, UK) V.1- 1960-
Volume(issue)/page/year: 14,534,1967
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE : 10 mg/kg
SEX/DURATION : female 15-16 day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death,
e.g., stunted fetus)
Reproductive - Effects on Embryo or Fetus - fetal death
REFERENCE :
PSEBAA Proceedings of the Society for Experimental Biology and Medicine.
(Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1903/04-
Volume(issue)/page/year: 113,124,1963
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE : 10 mg/kg
SEX/DURATION : female 11-12 day(s) after conception
TOXIC EFFECTS :
Reproductive - Fertility - post-implantation mortality (e.g. dead and/or
resorbed implants per total number of implants)
REFERENCE :
PSEBAA Proceedings of the Society for Experimental Biology and Medicine.
(Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1903/04-
Volume(issue)/page/year: 113,124,1963
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE : 500 mg/kg
SEX/DURATION : female 1-5 day(s) after conception
TOXIC EFFECTS :
Reproductive - Fertility - female fertility index (e.g. # females pregnant
per # sperm positive females; # females pregnant per # females mated)
REFERENCE :
JRPFA4 Journal of Reproduction and Fertility. (Biochemical Soc. Book Depot,
POB 32, Commerce Way, Colchester, Essex CO2 8HP, UK) V.1- 1960-
Volume(issue)/page/year: 14,534,1967
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - mouse
DOSE : 250 ug/kg
SEX/DURATION : female 3 day(s) after conception
TOXIC EFFECTS :
Reproductive - Fertility - female fertility index (e.g. # females pregnant
per # sperm positive females; # females pregnant per # females mated)
REFERENCE :
NATUAS Nature. (Nature Subscription Dept., POB 1018, Manasguan, NJ 08736)
V.1- 1869- Volume(issue)/page/year: 176,249,1955
** MUTATION DATA **
TYPE OF TEST : Mutation test systems - not otherwise specified
TEST SYSTEM : Non-mammalian species Cells - not otherwise specified
DOSE/DURATION : 50 nmol/L
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
37,3071,1977
TYPE OF TEST : DNA inhibition
TEST SYSTEM : Human HeLa cell
DOSE/DURATION : 10 umol/L
REFERENCE :
BICHAW Biochemistry. (American Chemical Soc., Distribution Office Dept.
223, POB 57136, West End Stn., Washington, DC 20037) V.1- 1962-
Volume(issue)/page/year: 15,5435,1976
TYPE OF TEST : Mutation test systems - not otherwise specified
TEST SYSTEM : Human HeLa cell
DOSE/DURATION : 5 umol/L
REFERENCE :
BICHAW Biochemistry. (American Chemical Soc., Distribution Office Dept.
223, POB 57136, West End Stn., Washington, DC 20037) V.1- 1962-
Volume(issue)/page/year: 15,5435,1976
TYPE OF TEST : Micronucleus test
TEST SYSTEM : Rodent - mouse Cells - not otherwise specified
DOSE/DURATION : 1 mg/L
REFERENCE :
JNCIAM Journal of the National Cancer Institute. (Washington, DC) V.1-60,
1940-78. For publisher information, see JJIND8. Volume(issue)/page/year:
56,357,1976
TYPE OF TEST : Mutation test systems - not otherwise specified
TEST SYSTEM : Rodent - mouse Cells - not otherwise specified
DOSE/DURATION : 10 umol/L
REFERENCE :
JMCMAR Journal of Medicinal Chemistry. (American Chemical Soc.,
Distribution Office Dept. 223, POB POB 57136, West End Stn., Washington, DC
20037) V.6- 1963- Volume(issue)/page/year: 19,148,1976
TYPE OF TEST : Micronucleus test
TEST SYSTEM : Rodent - hamster Lung
DOSE/DURATION : 250 nmol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 392,151,1997
TYPE OF TEST : Sex chromosome loss and nondisjunction
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - hamster
DOSE/DURATION : 160 ug/kg
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 327,237,1995
TYPE OF TEST : DNA inhibition
TEST SYSTEM : Mammal - species unspecified Ascites tumor
DOSE/DURATION : 50 umol/L
REFERENCE :
PAACA3 Proceedings of the American Association for Cancer Research. (Waverly
Press, 428 E. Preston St., Baltimore, MD 21202) V.1- 1954-
Volume(issue)/page/year: 24,309,1983
*** REVIEWS ***
TOXICOLOGY REVIEW
PMDCAY Progress in Medical Chemistry. (Elsevier Science Pub. Co., Inc., 52
Vanderbilt Ave., New York, NY 10017) V.1- 1961- Volume(issue)/page/year:
10,85,1974
TOXICOLOGY REVIEW
32XPAD "Teratology," Berry, C.L., and D.E. Poswillo, eds., New York,
Springer, 1975 Volume(issue)/page/year: -,49,1975
TOXICOLOGY REVIEW
ARVPAX Annual Review of Pharmacology. (Palo Alto, CA) V.1-15, 1961-75. For
publisher information, see ARPTDI. Volume(issue)/page/year: 5,447,1965
*** STATUS IN U.S. ***
EPA GENETOX PROGRAM 1988, Inconclusive: CHO gene mutation
*** END OF RECORD ***