*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : AR7678000
CHEMICAL NAME : Acridine,
6-chloro-9-(3-((2-chloroethyl)amino)propyl)amino-2-me
thoxy-, dihydrochloride
CAS REGISTRY NUMBER : 17070-45-0
LAST UPDATED : 199410
DATA ITEMS CITED : 41
MOLECULAR FORMULA : C19-H21-Cl2-N3-O.2Cl-H
MOLECULAR WEIGHT : 451.25
WISWESSER LINE NOTATION : T C666 BNJ EG IM3M2G LO1 &GH 2
COMPOUND DESCRIPTOR : Mutagen
SYNONYMS/TRADE NAMES :
* ICR-191
* ICR-191A
* ICR dihydrochloride
* 2-Methoxy-6-chloro-9-(3-(2-chloroethyl)aminopropylamino) acridine,
dihydrochloride
* 1,3-Propanediamine,
N-(2-chloroethyl)-N'-(6-chloro-2-methoxy-9-acridinyl)-, dihydrochloride
*** HEALTH HAZARD DATA ***
** ACUTE TOXICITY DATA **
TYPE OF TEST : LD20 - Lethal Dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 41 mg/kg
TOXIC EFFECTS :
Tumorigenic - active as anti-cancer agent
REFERENCE :
JMCMAR Journal of Medicinal Chemistry. (American Chemical Soc.,
Distribution Office Dept. 223, POB POB 57136, West End Stn., Washington, DC
20037) V.6- 1963- Volume(issue)/page/year: 15,739,1972
** MUTATION DATA **
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 320 ng/plate
REFERENCE :
EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E.
11th St., New York, NY 10003) V.10- 1987- Volume(issue)/page/year:
16,204,1990
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 500 ng/plate
REFERENCE :
PNASA6 Proceedings of the National Academy of Sciences of the United States
of America. (National Academy of Sciences, Printing & Pub. Office, 2101
Constitution Ave., Washington, DC 20418) V.1- 1915-
Volume(issue)/page/year: 72,5135,1975
TYPE OF TEST : Mutation test systems - not otherwise specified
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 220 ug/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 192,239,1987
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 93 umol/L
REFERENCE :
MGGEAE Molecular and General Genetics. (Springer-Verlag, Heidelberger Pl.
3, D-1000 Berlin 33, Fed. Rep. Ger.) V.99- 1967-
Volume(issue)/page/year: 195,418,1984
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 400 ng/plate
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 151,49,1985
TYPE OF TEST : Mutation test systems - not otherwise specified
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 700 nmol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 203,81,1988
TYPE OF TEST : DNA repair
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 160 ng/plate
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 158,19,1985
TYPE OF TEST : Phage inhibition capacity
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 300 ng/plate
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
43,2819,1983
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Bacillus subtilis
DOSE/DURATION : 10 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 38,271,1976
TYPE OF TEST : DNA repair
TEST SYSTEM : Bacteria - Bacillus subtilis
DOSE/DURATION : 125 ug/disc
REFERENCE :
AEMIDF Applied and Environmental Microbiology. (American Soc. for
Microbiology, 1913 I St., NW, Washington, DC 20006) V.31- 1976-
Volume(issue)/page/year: 43,177,1982
TYPE OF TEST : DNA damage
TEST SYSTEM : Bacteria - Bacillus subtilis
DOSE/DURATION : 1 mmol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 42,175,1977
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Serratia marcescens
DOSE/DURATION : 1 gm/L
REFERENCE :
ATXKA8 Archiv fuer Toxikologie. (Berlin, Fed. Rep. Ger.) V.15-31, 1954-74.
For publisher information, see ARTODN. Volume(issue)/page/year: 28,93,1971
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Haemophilus influenzae
DOSE/DURATION : 90900 ug/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 80,239,1981
TYPE OF TEST : DNA inhibition
TEST SYSTEM : Bacteria - Haemophilus influenzae
DOSE/DURATION : 250 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 80,249,1981
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Microorganism - not otherwise specified
DOSE/DURATION : 25 mg/L
REFERENCE :
GENRA8 Genetical Research. (Cambridge Univ. Press, 32 E. 57th St., New
York, NY 10022) V.1- 1960- Volume(issue)/page/year: 13,57,1969
TYPE OF TEST : DNA damage
TEST SYSTEM : Microorganism - not otherwise specified
DOSE/DURATION : 2 umol/L
REFERENCE :
EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E.
11th St., New York, NY 10003) V.10- 1987- Volume(issue)/page/year:
22,46,1993
TYPE OF TEST : Gene conversion and mitotic recombination
TEST SYSTEM : Yeast - Saccharomyces cerevisiae
DOSE/DURATION : 10 umol/L
REFERENCE :
AGACBH Agents and Actions, A Swiss Journal of Pharmacology. (Birkhaeuser
Verlag, POB 133, CH-4010 Basel, Switzerland) V.1- 1969/70-
Volume(issue)/page/year: 3,99,1973
TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Human Fibroblast
DOSE/DURATION : 1 umol/L/3H
REFERENCE :
IJCNAW International Journal of Cancer. (International Union Against
Cancer, 3 rue du Conseil- General, 1205 Geneva, Switzerland) V.1- 1966-
Volume(issue)/page/year: 16,284,1975
TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Human Lymphocyte
DOSE/DURATION : 4 mg/L/24H
REFERENCE :
IMNGBK Immunogenetics. (Springer-Verlag New York, Inc., Service Center, 44
Hartz Way, Secaucus, NJ 07094) V.1- 1974- Volume(issue)/page/year:
4,437,1977
TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Human Fibroblast
DOSE/DURATION : 300 nmol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 125,95,1984
TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Rodent - rat Liver
DOSE/DURATION : 10 umol/L
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 3,11,1981
TYPE OF TEST : Micronucleus test
TEST SYSTEM : Rodent - mouse Lymphocyte
DOSE/DURATION : 50 ug/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 222,191,1989
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Rodent - mouse Bone marrow
DOSE/DURATION : 250 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 81,215,1981
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Rodent - mouse Lymphocyte
DOSE/DURATION : 2 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 95,297,1982
TYPE OF TEST : Morphological transformation
TEST SYSTEM : Rodent - mouse Embryo
DOSE/DURATION : 580 nmol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 152,113,1985
TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Rodent - mouse Testis
DOSE/DURATION : 500 nmol/L/5H
REFERENCE :
ARTODN Archives of Toxicology. (Springer-Verlag, Heidelberger Pl. 3, D-1000
Berlin 33, Fed. Rep. Ger.) V.32- 1974- Volume(issue)/page/year:
37,195,1977
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - mouse Lymphocyte
DOSE/DURATION : 250 ug/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 222,191,1989
TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Rodent - mouse Liver
DOSE/DURATION : 500 ug/L
REFERENCE :
SCGTDW Somatic Cell Genetics. (New York, NY) V.1-9, 1975-83. For
publisher information, see SCMGDN. Volume(issue)/page/year: 7,373,1981
TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Rodent - mouse Leukocyte
DOSE/DURATION : 2200 nmol/L
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
42,956,1982
TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Rodent - mouse Lymphocyte
DOSE/DURATION : 2200 nmol/L
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
42,956,1982
TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Rodent - mouse Cells - not otherwise specified
DOSE/DURATION : 2400 ug/L
REFERENCE :
43VMAC "Microsomes, Drug Oxidations, and Chemical Carcinogenesis,
International Symposium on Microsomes and Drug Oxidations, 4th, 1979," Coon,
M.J., et al., eds., New York, Academic Press, Inc., 1980
Volume(issue)/page/year: 4,1149,1980
TYPE OF TEST : Host-mediated assay
TEST SYSTEM : Rodent - mouse Yeast - Saccharomyces cerevisiae
DOSE/DURATION : 500 umol/L/kg
REFERENCE :
AGACBH Agents and Actions, A Swiss Journal of Pharmacology. (Birkhaeuser
Verlag, POB 133, CH-4010 Basel, Switzerland) V.1- 1969/70-
Volume(issue)/page/year: 3,99,1973
TYPE OF TEST : Host-mediated assay
TEST SYSTEM : Rodent - mouse Bacteria - Salmonella typhimurium
DOSE/DURATION : 46 mg/kg
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 156,93,1985
TYPE OF TEST : Sister chromatid exchange
TEST SYSTEM : Rodent - hamster Lung
DOSE/DURATION : 10 mg/L
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
44,3270,1984
TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Rodent - hamster Ovary
DOSE/DURATION : 500 ug/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 139,95,1984
TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Rodent - hamster Lung
DOSE/DURATION : 1 umol/L
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
42,817,1982
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Amphibian - frog Embryo
DOSE/DURATION : 1 umol/L
REFERENCE :
JCLLAX Journal of Cellular Physiology. (Alan R. Liss, Inc., 41 E. 11th St.,
New York, NY 10003) V.67- 1966- Volume(issue)/page/year: 94,187,1978
*** STATUS IN U.S. ***
EPA GENETOX PROGRAM 1988, Positive: Host-mediated assay; Histidine
reversion-Ames test
EPA GENETOX PROGRAM 1988, Positive: S cerevisiae gene conversion
EPA GENETOX PROGRAM 1988, Negative: In vitro UDS-human fibroblast
*** END OF RECORD ***