*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : AR8670000
CHEMICAL NAME : Acridine, 3,6-diamino-
CAS REGISTRY NUMBER : 92-62-6
BEILSTEIN REFERENCE NO. : 0166050
REFERENCE : 5-22-11-00322 (Beilstein Handbook Reference)
LAST UPDATED : 199806
DATA ITEMS CITED : 42
MOLECULAR FORMULA : C13-H11-N3
MOLECULAR WEIGHT : 209.27
WISWESSER LINE NOTATION : T C666 BNJ EZ MZ
COMPOUND DESCRIPTOR : Tumorigen
Drug
Mutagen
SYNONYMS/TRADE NAMES :
* 3,6-Acridinediamine
* 2,8-Diaminoacridine
* 3,6-Diaminoacridine
* 2,8-Diaminoacridinium
* 3,6-Diaminoacridinium
* 3,7-Diamino-5-azaanthracene
* Isoflav base
* Proflavin
* Proflavine
*** HEALTH HAZARD DATA ***
** ACUTE TOXICITY DATA **
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : >1 gm/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
JMCMAR Journal of Medicinal Chemistry. (American Chemical Soc.,
Distribution Office Dept. 223, POB POB 57136, West End Stn., Washington, DC
20037) V.6- 1963- Volume(issue)/page/year: 18,320,1975
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 50 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
CHTHBK Chemotherapy (Basel). (S. Karger Pub., Inc., 79 Fifth Ave., New
York, NY 10003) V.13- 1968- Volume(issue)/page/year: 16,371,1971
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 140 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
BJEPA5 British Journal of Experimental Pathology. (Blackwell Scientific
Pub. Ltd., POB 88, Oxford, UK) V.1- 1920- Volume(issue)/page/year:
28,1,1947
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Mammal - cat
DOSE/DURATION : 11111 ug/kg
TOXIC EFFECTS :
Sense Organs and Special Senses (Olfaction) - effect, not otherwise
specified
Sense Organs and Special Senses (Eye) - effect, not otherwise specified
Lungs, Thorax, or Respiration - other changes
REFERENCE :
LANCAO Lancet. (7 Adam St., London WC2N 6AD, UK) V.1- 1823-
Volume(issue)/page/year: 196,838,1919
** MUTATION DATA **
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 1 ug/plate
REFERENCE :
CPBTAL Chemical and Pharmaceutical Bulletin. (Japan Pub. Trading Co., USA,
1255 Howard St., San Francisco, CA 94103) V.6- 1958-
Volume(issue)/page/year: 35,792,1987
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 10 ug/plate
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 25,187,1974
TYPE OF TEST : DNA repair
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 1 mg/plate
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 54,101,1978
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Microorganism - not otherwise specified
DOSE/DURATION : 4 mg/L
REFERENCE :
JMOBAK Journal of Molecular Biology. (Academic Press, Inc., 1 E. First St.,
Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year: 3,762,1961
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Microorganism - not otherwise specified
DOSE/DURATION : 7500 ug/L
REFERENCE :
JOVIAM Journal of Virology. (American Soc. for Microbiology, 1913 I St.,
NW, Washington, DC 20006) V.1- 1967- Volume(issue)/page/year:
16,1000,1975
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Microorganism - not otherwise specified
DOSE/DURATION : 10 umol/L
REFERENCE :
GENTAE Genetics. (Genetics Business Office, POB 64025, Baltimore, MD 21264)
V.1- 1916- Volume(issue)/page/year: 90,1,1978
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Microorganism - not otherwise specified
DOSE/DURATION : 5 mg/L
REFERENCE :
VIRLAX Virology. (Academic Press, Inc., 1 E. First St., Duluth, MN 55802)
V.1- 1955- Volume(issue)/page/year: 37,335,1969
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Yeast - Saccharomyces cerevisiae
DOSE/DURATION : 25 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 82,87,1981
TYPE OF TEST : Gene conversion and mitotic recombination
TEST SYSTEM : Yeast - Saccharomyces cerevisiae
DOSE/DURATION : 1 umol/L
REFERENCE :
MGGEAE Molecular and General Genetics. (Springer-Verlag, Heidelberger Pl.
3, D-1000 Berlin 33, Fed. Rep. Ger.) V.99- 1967-
Volume(issue)/page/year: 144,131,1976
TYPE OF TEST : Specific locus test
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Insect - silkworm
DOSE/DURATION : 50 mg
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 22,295,1974
TYPE OF TEST : DNA damage
TEST SYSTEM : Fish - salmon Sperm
DOSE/DURATION : 100 mmol
REFERENCE :
ABBIA4 Archives of Biochemistry and Biophysics. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.31- 1951- Volume(issue)/page/year:
120,292,1967
TYPE OF TEST : DNA inhibition
TEST SYSTEM : Human Fibroblast
DOSE/DURATION : 10 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 183,89,1987
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Human HeLa cell
DOSE/DURATION : 1 mg/L/1H
REFERENCE :
ECREAL Experimental Cell Research. (Academic Press, Inc., 1 E. First St.,
Duluth, MN 55802) V.10- 1950- Volume(issue)/page/year: 39,296,1965
TYPE OF TEST : Morphological transformation
TEST SYSTEM : Rodent - rat Embryo
DOSE/DURATION : 370 ng/plate
REFERENCE :
JJATDK JAT, Journal of Applied Toxicology. (John Wiley & Sons Ltd., Baffins
Lane, Chichester, W. Sussex PO19 1UD, UK) V.1- 1981-
Volume(issue)/page/year: 1,190,1981
TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Rodent - rat Liver
DOSE/DURATION : 1 umol/L
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 3,11,1981
TYPE OF TEST : Morphological transformation
TEST SYSTEM : Rodent - mouse Embryo
DOSE/DURATION : 1 umol/L
REFERENCE :
MOPMA3 Molecular Pharmacology. (Williams & Wilkins Co., 428 E. Preston St.,
Baltimore, MD 21202) V.1- 1965- Volume(issue)/page/year: 21,739,1982
TYPE OF TEST : DNA damage
TEST SYSTEM : Rodent - mouse Ascites tumor
DOSE/DURATION : 48 umol/L
REFERENCE :
BBACAQ Biochimica et Biophysica Acta. (Elsevier Science Pub. B.V., POB 211,
1000 AE Amsterdam, Netherlands) V.1- 1947- Volume(issue)/page/year:
374,96,1974
TYPE OF TEST : DNA inhibition
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 100 mg/kg
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 46,305,1977
TYPE OF TEST : DNA inhibition
TEST SYSTEM : Rodent - mouse Embryo
DOSE/DURATION : 1 umol/L
REFERENCE :
MOPMA3 Molecular Pharmacology. (Williams & Wilkins Co., 428 E. Preston St.,
Baltimore, MD 21202) V.1- 1965- Volume(issue)/page/year: 21,739,1982
TYPE OF TEST : Mutation test systems - not otherwise specified
TEST SYSTEM : Rodent - mouse Embryo
DOSE/DURATION : 1 umol/L
REFERENCE :
MOPMA3 Molecular Pharmacology. (Williams & Wilkins Co., 428 E. Preston St.,
Baltimore, MD 21202) V.1- 1965- Volume(issue)/page/year: 21,739,1982
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Lung
DOSE/DURATION : 1 mg/L
REFERENCE :
JNCIAM Journal of the National Cancer Institute. (Washington, DC) V.1-60,
1940-78. For publisher information, see JJIND8. Volume(issue)/page/year:
59,289,1977
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Fibroblast
DOSE/DURATION : 4 umol/L
REFERENCE :
ECREAL Experimental Cell Research. (Academic Press, Inc., 1 E. First St.,
Duluth, MN 55802) V.10- 1950- Volume(issue)/page/year: 85,239,1974
TYPE OF TEST : Sister chromatid exchange
TEST SYSTEM : Rodent - hamster Lung
DOSE/DURATION : 1 mg/L
REFERENCE :
JNCIAM Journal of the National Cancer Institute. (Washington, DC) V.1-60,
1940-78. For publisher information, see JJIND8. Volume(issue)/page/year:
59,289,1977
TYPE OF TEST : Sister chromatid exchange
TEST SYSTEM : Rodent - hamster Ovary
DOSE/DURATION : 20 ug/L
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 4,65,1982
TYPE OF TEST : Sister chromatid exchange
TEST SYSTEM : Rodent - hamster Fibroblast
DOSE/DURATION : 1 umol/L
REFERENCE :
ECREAL Experimental Cell Research. (Academic Press, Inc., 1 E. First St.,
Duluth, MN 55802) V.10- 1950- Volume(issue)/page/year: 85,239,1974
TYPE OF TEST : DNA damage
TEST SYSTEM : Mammal - species unspecified Lymphocyte
DOSE/DURATION : 13500 nmol/L
REFERENCE :
ZEKBAI Zeitschrift fuer Krebsforschung. (Berlin, Fed. Rep. Ger.) V.1-75,
1903-71. For publisher information, see JCROD7. Volume(issue)/page/year:
67,127,1965
*** REVIEWS ***
IARC Cancer Review:Animal Inadequate Evidence
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 24,195,1980
IARC Cancer Review:Human No Adequate Data
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 24,195,1980
*** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA ***
NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA :
NOES - National Occupational Exposure Survey (1983)
NOES Hazard Code - X6466
No. of Facilities: 7 (estimated)
No. of Industries: 1
No. of Occupations: 2
No. of Employees: 628 (estimated)
No. of Female Employees: 621 (estimated)
*** STATUS IN U.S. ***
EPA GENETOX PROGRAM 1988, Positive: D melanogaster-whole sex chrom. loss
EPA GENETOX PROGRAM 1988, Positive: L5178Y cells in vitro-TK test; E coli
polA without S9
EPA GENETOX PROGRAM 1988, Positive: Histidine reversion-Ames test
EPA GENETOX PROGRAM 1988, Positive/dose response: In vitro SCE-nonhuman
EPA GENETOX PROGRAM 1988, Negative: In vivo SCE-nonhuman
EPA GENETOX PROGRAM 1988, Inconclusive: Carcinogenicity-mouse/rat
EPA GENETOX PROGRAM 1988, Inconclusive: D melanogaster-nondisjunction
EPA GENETOX PROGRAM 1988, Inconclusive: S cerevisiae gene conversion
EPA TSCA Section 8(b) CHEMICAL INVENTORY
*** END OF RECORD ***