*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : AR9625000
CHEMICAL NAME : Acridinium, 3,6-diamino-10-methyl-, chloride
CAS REGISTRY NUMBER : 86-40-8
LAST UPDATED : 199806
DATA ITEMS CITED : 42
MOLECULAR FORMULA : C14-H14-N3.Cl
MOLECULAR WEIGHT : 259.76
WISWESSER LINE NOTATION : T C666 BKJ B1 EZ MZ &Q &G
COMPOUND DESCRIPTOR : Drug
Mutagen
Human
SYNONYMS/TRADE NAMES :
* Avlon
* Burnol
* Chromoflavine
* C.I. 46000
* 3,6-Diamino-10-methylacridinium chloride
*** HEALTH HAZARD DATA ***
** ACUTE TOXICITY DATA **
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Human
DOSE/DURATION : 1500 ug/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
JPETAB Journal of Pharmacology and Experimental Therapeutics. (Williams &
Wilkins Co., 428 E. Preston St., Baltimore, MD 21202) V.1- 1909/10-
Volume(issue)/page/year: 38,145,1930
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 11 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
23,288,1972
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 14 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
BJEPA5 British Journal of Experimental Pathology. (Blackwell Scientific
Pub. Ltd., POB 88, Oxford, UK) V.1- 1920- Volume(issue)/page/year:
28,1,1947
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 40 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
BJEPA5 British Journal of Experimental Pathology. (Blackwell Scientific
Pub. Ltd., POB 88, Oxford, UK) V.1- 1920- Volume(issue)/page/year:
28,1,1947
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Mammal - dog
DOSE/DURATION : 25 mg/kg
TOXIC EFFECTS :
Behavioral - convulsions or effect on seizure threshold
Vascular - BP lowering not characterized in autonomic section
Lungs, Thorax, or Respiration - other changes
REFERENCE :
JPETAB Journal of Pharmacology and Experimental Therapeutics. (Williams &
Wilkins Co., 428 E. Preston St., Baltimore, MD 21202) V.1- 1909/10-
Volume(issue)/page/year: 38,145,1930
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Mammal - cat
DOSE/DURATION : 7353 ug/kg
TOXIC EFFECTS :
Sense Organs and Special Senses (Olfaction) - effect, not otherwise
specified
Sense Organs and Special Senses (Eye) - effect, not otherwise specified
Lungs, Thorax, or Respiration - other changes
REFERENCE :
LANCAO Lancet. (7 Adam St., London WC2N 6AD, UK) V.1- 1823-
Volume(issue)/page/year: 196,838,1919
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - rabbit
DOSE/DURATION : 25 mg/kg
TOXIC EFFECTS :
Behavioral - convulsions or effect on seizure threshold
Vascular - BP lowering not characterized in autonomic section
Lungs, Thorax, or Respiration - other changes
REFERENCE :
JPETAB Journal of Pharmacology and Experimental Therapeutics. (Williams &
Wilkins Co., 428 E. Preston St., Baltimore, MD 21202) V.1- 1909/10-
Volume(issue)/page/year: 38,145,1930
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - guinea pig
DOSE/DURATION : 250 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
HBAMAK "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." (Leipzig,
Ger. Dem. Rep.) Volume(issue)/page/year: 4,1292,1935
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - guinea pig
DOSE/DURATION : 250 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
HBAMAK "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." (Leipzig,
Ger. Dem. Rep.) Volume(issue)/page/year: 4,1292,1935
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - guinea pig
DOSE/DURATION : 40 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
HBAMAK "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." (Leipzig,
Ger. Dem. Rep.) Volume(issue)/page/year: 4,1292,1935
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Bird - quail
DOSE/DURATION : >100 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
EESADV Ecotoxicology and Environmental Safety. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1977- Volume(issue)/page/year:
6,149,1982
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Amphibian - frog
DOSE/DURATION : 800 mg/kg
TOXIC EFFECTS :
Peripheral Nerve and Sensation - spastic paralysis with or without sensory
change
Cardiac - other changes
Lungs, Thorax, or Respiration - other changes
REFERENCE :
ZGEMAZ Zeitschrift fuer die Gesamte Experimentelle Medizin. (Berlin, Fed.
Rep. Ger.) V.1-139, 1913-65. For publisher information, see REXMAS.
Volume(issue)/page/year: 12,195,1921
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Mammal - species unspecified
DOSE/DURATION : 65 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
JPETAB Journal of Pharmacology and Experimental Therapeutics. (Williams &
Wilkins Co., 428 E. Preston St., Baltimore, MD 21202) V.1- 1909/10-
Volume(issue)/page/year: 80,217,1944
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Bird - wild bird species
DOSE/DURATION : 56200 ug/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
AECTCV Archives of Environmental Contamination and Toxicology.
(Springer-Verlag New York, Inc., Service Center, 44 Hartz Way, Secaucus, NJ
070944) V.1- 1973- Volume(issue)/page/year: 12,355,1983
** MUTATION DATA **
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 1 ug/plate
REFERENCE :
CPBTAL Chemical and Pharmaceutical Bulletin. (Japan Pub. Trading Co., USA,
1255 Howard St., San Francisco, CA 94103) V.6- 1958-
Volume(issue)/page/year: 35,792,1987
TYPE OF TEST : DNA repair
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 2000 ppm
REFERENCE :
AGACBH Agents and Actions, A Swiss Journal of Pharmacology. (Birkhaeuser
Verlag, POB 133, CH-4010 Basel, Switzerland) V.1- 1969/70-
Volume(issue)/page/year: 4,286,1974
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 15 ppm/3H
REFERENCE :
AMNTA4 American Naturalist. (Univ. of Chicago Press, Journals Div., POB
37005, Chicago, IL 60637) V.1- 1867- Volume(issue)/page/year:
85,119,1951
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 10 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 140,13,1984
TYPE OF TEST : DNA repair
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 2000 ppm
REFERENCE :
AGACBH Agents and Actions, A Swiss Journal of Pharmacology. (Birkhaeuser
Verlag, POB 133, CH-4010 Basel, Switzerland) V.1- 1969/70-
Volume(issue)/page/year: 4,286,1974
TYPE OF TEST : DNA adduct
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 5 mg/L
REFERENCE :
MGGEAE Molecular and General Genetics. (Springer-Verlag, Heidelberger Pl.
3, D-1000 Berlin 33, Fed. Rep. Ger.) V.99- 1967-
Volume(issue)/page/year: 122,89,1973
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Bacillus subtilis
DOSE/DURATION : 6 mg/L/2H (Continuous)
REFERENCE :
CJMIAZ Canadian Journal of Microbiology. (National Research Council of
Canada, Publication Sales and Distribution, Ottawa ON K1A OR6, Canada) V.1-
1954- Volume(issue)/page/year: 14,61,1968
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Serratia marcescens
DOSE/DURATION : 1 gm/L
REFERENCE :
ATXKA8 Archiv fuer Toxikologie. (Berlin, Fed. Rep. Ger.) V.15-31, 1954-74.
For publisher information, see ARTODN. Volume(issue)/page/year: 28,93,1971
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Microorganism - not otherwise specified
DOSE/DURATION : 15 mg/L
REFERENCE :
GENRA8 Genetical Research. (Cambridge Univ. Press, 32 E. 57th St., New
York, NY 10022) V.1- 1960- Volume(issue)/page/year: 13,57,1969
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Microorganism - not otherwise specified
DOSE/DURATION : 20 ppm
REFERENCE :
APMBAY Applied Microbiology. (Washington, DC) V.1-30, 1953-75. For
publisher information, see AEMIDF. Volume(issue)/page/year: 6,45,1958
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Microorganism - not otherwise specified
DOSE/DURATION : 2 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 35,65,1976
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Microorganism - not otherwise specified
DOSE/DURATION : 100 mg/L/2D
REFERENCE :
JULRA7 Journal of Ultrastructure Research. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1975- Volume(issue)/page/year:
25,37,1968
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Microorganism - not otherwise specified
DOSE/DURATION : 107 mg/L
REFERENCE :
MIBLAO Microbiology (Moscow). (Plenum Pub. Corp., 233 Spring St., New York,
NY 10013) V.26- 1957- Volume(issue)/page/year: 42,442,1973
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Microorganism - not otherwise specified
DOSE/DURATION : 20 mg/plate
REFERENCE :
EJMBA2 Egyptian Journal of Microbiology. (National Information and
Documentation Centre, A1-Tahrir St., Awqaf P.O. Dokki, Cairo, Egypt) V.7-
1972- Volume(issue)/page/year: 16,133,1981
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Microorganism - not otherwise specified
DOSE/DURATION : 4 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 43,37,1977
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Yeast - Saccharomyces cerevisiae
DOSE/DURATION : 2000 ppm
REFERENCE :
AGACBH Agents and Actions, A Swiss Journal of Pharmacology. (Birkhaeuser
Verlag, POB 133, CH-4010 Basel, Switzerland) V.1- 1969/70-
Volume(issue)/page/year: 4,286,1974
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Yeast - Saccharomyces cerevisiae
DOSE/DURATION : 30 mg/L
REFERENCE :
MUTAEX Mutagenesis. (Oxford Univ. Press, Pinkhill House, Southfield Road,
Eynsham, Oxford OX8 1JJ, UK) V.1- 1986- Volume(issue)/page/year: 4,335,1989
TYPE OF TEST : Gene conversion and mitotic recombination
TEST SYSTEM : Yeast - Saccharomyces cerevisiae
DOSE/DURATION : 2000 ppm
REFERENCE :
AGACBH Agents and Actions, A Swiss Journal of Pharmacology. (Birkhaeuser
Verlag, POB 133, CH-4010 Basel, Switzerland) V.1- 1969/70-
Volume(issue)/page/year: 4,286,1974
TYPE OF TEST : DNA damage
TEST SYSTEM : Yeast - Saccharomyces cerevisiae
DOSE/DURATION : 50 ppm
REFERENCE :
SCIEAS Science. (American Assoc. for the Advancement of Science, 1333 H
St., NW, Washington, DC 20005) V.1- 1895- Volume(issue)/page/year:
163,1331,1969
TYPE OF TEST : Micronucleus test
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 500 mg/kg/5D (Continuous)
REFERENCE :
CNJGA8 Canadian Journal of Genetics and Cytology. (National Research
Council of Canada, Publication Sales and Distribution, Ottawa ON K1A OR6,
Canada) V.1- 1959- Volume(issue)/page/year: 21,319,1979
TYPE OF TEST : DNA damage
TEST SYSTEM : Rodent - mouse Mammary gland
DOSE/DURATION : 100 nmol/L
REFERENCE :
JOBIAO Journal of Biochemistry (Tokyo). (Japanese Biochemical Soc., c/o
Japan Academic Soc. Center, 2-4-16 Yayoi, Bunkyo-ku, Tokyo 113, Japan) V.1-
1922- Volume(issue)/page/year: 84,145,1978
TYPE OF TEST : Cytogenetic analysis
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 100 mg/kg/50D (Intermittent)
REFERENCE :
HUGEDQ Human Genetics. (Springer-Verlag New York, Inc., Service Center, 44
Hartz Way, Secaucus, NJ 07094) V.31- 1976- Volume(issue)/page/year:
40,87,1977
TYPE OF TEST : Dominant lethal test
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 50 mg/kg
REFERENCE :
HUGEDQ Human Genetics. (Springer-Verlag New York, Inc., Service Center, 44
Hartz Way, Secaucus, NJ 07094) V.31- 1976- Volume(issue)/page/year:
40,87,1977
TYPE OF TEST : Sperm Morphology
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 250 mg/kg/5D (Continuous)
REFERENCE :
CNJGA8 Canadian Journal of Genetics and Cytology. (National Research
Council of Canada, Publication Sales and Distribution, Ottawa ON K1A OR6,
Canada) V.1- 1959- Volume(issue)/page/year: 21,319,1979
TYPE OF TEST : DNA adduct
TEST SYSTEM : Rodent - hamster Cells - not otherwise specified
DOSE/DURATION : 11 ug/L
REFERENCE :
ECREAL Experimental Cell Research. (Academic Press, Inc., 1 E. First St.,
Duluth, MN 55802) V.10- 1950- Volume(issue)/page/year: 53,101,1968
TYPE OF TEST : DNA adduct
TEST SYSTEM : Mammal - species unspecified Lymphocyte
DOSE/DURATION : 10 pph
REFERENCE :
BIPMAA Biopolymers. (John Wiley & Sons, Inc., 605 Third Ave., New York, NY
10158) V.1- 1963- Volume(issue)/page/year: 11,2537,1972
*** REVIEWS ***
TOXICOLOGY REVIEW
32XPAD "Teratology," Berry, C.L., and D.E. Poswillo, eds., New York,
Springer, 1975 Volume(issue)/page/year: -,49,1975
*** STATUS IN U.S. ***
EPA TSCA Section 8(b) CHEMICAL INVENTORY
*** END OF RECORD ***