*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : MH6125000
CHEMICAL NAME : Heliotrine
CAS REGISTRY NUMBER : 303-33-3
BEILSTEIN REFERENCE NO. : 0292841
LAST UPDATED : 199803
DATA ITEMS CITED : 31
MOLECULAR FORMULA : C16-H27-N-O5
MOLECULAR WEIGHT : 313.44
WISWESSER LINE NOTATION : T55 AN CUTJ D1OVXQY1&1&Y1&O1 FQ
COMPOUND DESCRIPTOR : Tumorigen
Drug
Mutagen
Reproductive Effector
Natural Product
SYNONYMS/TRADE NAMES :
* (+)-Heliotrine
* 7S-Heliotrine
* Heliotron
*** HEALTH HAZARD DATA ***
** ACUTE TOXICITY DATA **
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 50 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
NATUAS Nature. (Nature Subscription Dept., POB 1018, Manasguan, NJ 08736)
V.1- 1869- Volume(issue)/page/year: 179,361,1957
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 296 mg/kg
TOXIC EFFECTS :
Gastrointestinal - hypermotility, diarrhea
Gastrointestinal - other changes
Kidney, Ureter, Bladder - hematuria
REFERENCE :
JPBAA7 Journal of Pathology and Bacteriology. (London, UK) V.1-96,
1892-1968. For publisher information, see JPTLAS. Volume(issue)/page/year:
75,17,1958
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 274 mg/kg
TOXIC EFFECTS :
Liver - other changes
REFERENCE :
AMPLAO AMA Archives of Pathology. (Chicago, IL) V.50(4)-69, 1950-60. For
publisher information, see APLMAS. Volume(issue)/page/year: 64,152,1957
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 251 mg/kg
TOXIC EFFECTS :
Liver - other changes
REFERENCE :
AMPLAO AMA Archives of Pathology. (Chicago, IL) V.50(4)-69, 1950-60. For
publisher information, see APLMAS. Volume(issue)/page/year: 64,152,1957
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Mammal - species unspecified
DOSE/DURATION : 250 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
PAREAQ Pharmacological Reviews. (Williams & Wilkins, 428 E. Preston St.,
Baltimore, MD 21202) V.1- 1949- Volume(issue)/page/year: 22,429,1970
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Unreported
SPECIES OBSERVED : Mammal - species unspecified
DOSE/DURATION : 260 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
PAREAQ Pharmacological Reviews. (Williams & Wilkins, 428 E. Preston St.,
Baltimore, MD 21202) V.1- 1949- Volume(issue)/page/year: 22,429,1970
** OTHER MULTIPLE DOSE TOXICITY DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 900 mg/kg/9D-I
TOXIC EFFECTS :
Liver - hepatitis (hepatocellular necrosis), zonal
Blood - macrocytosis
Biochemical - Enzyme inhibition, induction, or change in blood or tissue
levels - transaminases
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
101,271,1989
** TUMORIGENIC DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 460 mg/kg/6D-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Gastrointestinal - tumors
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
35,2020,1975
** REPRODUCTIVE DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE : 50 mg/kg
SEX/DURATION : female 10 day(s) after conception
TOXIC EFFECTS :
Reproductive - Fertility - post-implantation mortality (e.g. dead and/or
resorbed implants per total number of implants)
REFERENCE :
BJEPA5 British Journal of Experimental Pathology. (Blackwell Scientific
Pub. Ltd., POB 88, Oxford, UK) V.1- 1920- Volume(issue)/page/year:
42,369,1961
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE : 100 mg/kg
SEX/DURATION : female 9 day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death,
e.g., stunted fetus)
Reproductive - Specific Developmental Abnormalities - musculoskeletal system
REFERENCE :
BJEPA5 British Journal of Experimental Pathology. (Blackwell Scientific
Pub. Ltd., POB 88, Oxford, UK) V.1- 1920- Volume(issue)/page/year:
42,369,1961
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE : 150 mg/kg
SEX/DURATION : female 16 day(s) after conception
TOXIC EFFECTS :
Reproductive - Specific Developmental Abnormalities - blood and lymphatic
systems (including spleen and marrow)
REFERENCE :
BJEPA5 British Journal of Experimental Pathology. (Blackwell Scientific
Pub. Ltd., POB 88, Oxford, UK) V.1- 1920- Volume(issue)/page/year:
42,369,1961
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE : 200 mg/kg
SEX/DURATION : female 12 day(s) after conception
TOXIC EFFECTS :
Reproductive - Specific Developmental Abnormalities - homeostasis
Reproductive - Specific Developmental Abnormalities - craniofacial
(including nose and tongue)
Reproductive - Specific Developmental Abnormalities - Central Nervous System
REFERENCE :
BJEPA5 British Journal of Experimental Pathology. (Blackwell Scientific
Pub. Ltd., POB 88, Oxford, UK) V.1- 1920- Volume(issue)/page/year:
42,369,1961
** MUTATION DATA **
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 1 mg/plate
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 68,211,1979
TYPE OF TEST : Cytogenetic analysis
ROUTE OF EXPOSURE : Parenteral
TEST SYSTEM : Insect - Drosophila melanogaster
DOSE/DURATION : 500 umol/L
REFERENCE :
ZEVBA5 Zeitschrift fuer Vererbungslehre. (Berlin, Fed. Rep. Ger.) V.89-98,
1958-66. For publisher information, see MGGEAE. Volume(issue)/page/year:
94,115,1963
TYPE OF TEST : Sex chromosome loss and nondisjunction
ROUTE OF EXPOSURE : Parenteral
TEST SYSTEM : Insect - Drosophila melanogaster
DOSE/DURATION : 20 umol/L
REFERENCE :
ZEVBA5 Zeitschrift fuer Vererbungslehre. (Berlin, Fed. Rep. Ger.) V.89-98,
1958-66. For publisher information, see MGGEAE. Volume(issue)/page/year:
91,74,1960
TYPE OF TEST : Sex chromosome loss and nondisjunction
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Insect - Drosophila melanogaster
DOSE/DURATION : 1 mmol/L
REFERENCE :
TCMUD8 Teratogenesis, Carcinogenesis, and Mutagenesis. (Alan R. Liss, Inc.,
41 E. 11th St., New York, NY 10003) V.1- 1980- Volume(issue)/page/year:
8,205,1988
TYPE OF TEST : Dominant lethal test
ROUTE OF EXPOSURE : Parenteral
TEST SYSTEM : Insect - Drosophila melanogaster
DOSE/DURATION : 1 mmol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 8,139,1969
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Mold - Aspergillus nidulans
DOSE/DURATION : 20 mmol/L/4H
REFERENCE :
NATUAS Nature. (Nature Subscription Dept., POB 1018, Manasguan, NJ 08736)
V.1- 1869- Volume(issue)/page/year: 210,593,1966
TYPE OF TEST : DNA inhibition
TEST SYSTEM : Human Liver
DOSE/DURATION : 25 mg/L
REFERENCE :
IJEVAW International Journal of Environmental Studies. (Gordon & Breach
Science Pub. Inc., c/o STBS Ltd., 1 Bedford St., London WCE 9PP, UK) V.1-
1970- Volume(issue)/page/year: 1,107,1971
TYPE OF TEST : Mutation test systems - not otherwise specified
TEST SYSTEM : Human Liver
DOSE/DURATION : 10 mg/L
REFERENCE :
IJEVAW International Journal of Environmental Studies. (Gordon & Breach
Science Pub. Inc., c/o STBS Ltd., 1 Bedford St., London WCE 9PP, UK) V.1-
1970- Volume(issue)/page/year: 1,107,1971
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Human Lymphocyte
DOSE/DURATION : 100 umol/L
REFERENCE :
PLMEAA Planta Medica. (Georg Thieme Verlag, Postfach 732, D-7000 Stuttgart
1, Fed. Rep. Ger.) V.1- 1953- Volume(issue)/page/year: 51,89,1985
TYPE OF TEST : Mutation test systems - not otherwise specified
ROUTE OF EXPOSURE : Unreported
TEST SYSTEM : Rodent - rat
DOSE/DURATION : 150 ug/kg
REFERENCE :
ANYAA9 Annals of the New York Academy of Sciences. (New York Academy of
Sciences, 2 E. 63rd St., New York, NY 10021) V.1- 1877-
Volume(issue)/page/year: 163,837,1969
TYPE OF TEST : Micronucleus test
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 225 mg/kg
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 285,27,1993
TYPE OF TEST : Sister chromatid exchange
TEST SYSTEM : Rodent - hamster Lung
DOSE/DURATION : 1200 ug/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 142,209,1985
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Mammal - species unspecified Leukocyte
DOSE/DURATION : 50 umol/L
REFERENCE :
AJBSAM Australian Journal of Biological Sciences. (Commonwealth Scientific
and Industrial Research Organization, 314 Albert St., E. Melbourne, Vic.
3002, Australia) V.6- 1953- Volume(issue)/page/year: 21,469,1968
*** REVIEWS ***
TOXICOLOGY REVIEW
32XPAD "Teratology," Berry, C.L., and D.E. Poswillo, eds., New York,
Springer, 1975 Volume(issue)/page/year: -,49,1975
*** STATUS IN U.S. ***
EPA GENETOX PROGRAM 1988, Positive: Aspergillus-forward mutation
EPA GENETOX PROGRAM 1988, Positive: D melanogaster-whole sex chrom. loss
EPA GENETOX PROGRAM 1988, Positive: D melanogaster Sex-linked lethal
EPA GENETOX PROGRAM 1988, Inconclusive: D melanogaster-nondisjunction
EPA GENETOX PROGRAM 1988, Inconclusive: D melanogaster-reciprocal
translocation
*** END OF RECORD ***