*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : MO3150000
CHEMICAL NAME : 2,5-Hexanedione
CAS REGISTRY NUMBER : 110-13-4
BEILSTEIN REFERENCE NO. : 0506525
REFERENCE : 4-01-00-03688 (Beilstein Handbook Reference)
LAST UPDATED : 199710
DATA ITEMS CITED : 39
MOLECULAR FORMULA : C6-H10-O2
MOLECULAR WEIGHT : 114.16
WISWESSER LINE NOTATION : 1V2V1
COMPOUND DESCRIPTOR : Mutagen
Reproductive Effector
Primary Irritant
SYNONYMS/TRADE NAMES :
* Acetone, acetonyl-
* Acetonyl acetone
* Diacetonyl
* alpha,beta-Diacetylethane
* 1,2-Diacetylethane
* 2,5-Diketohexane
*** HEALTH HAZARD DATA ***
** SKIN/EYE IRRITATION DATA **
TYPE OF TEST : Standard Draize test
ROUTE OF EXPOSURE : Administration into the eye
SPECIES OBSERVED : Rodent - rabbit
DOSE/DURATION : 100 mg
REACTION SEVERITY : Severe
REFERENCE :
AJOPAA American Journal of Ophthalmology. (Ophthalmic Pub. Co., 435 N.
Michigan Ave., Suite 1415, Chicago, IL 60611) Series 3: V.1- 1918-
Volume(issue)/page/year: 29,1363,1946
TYPE OF TEST : Standard Draize test
ROUTE OF EXPOSURE : Administration into the eye
SPECIES OBSERVED : Rodent - rabbit
DOSE/DURATION : 100 mg/24H
REACTION SEVERITY : Moderate
REFERENCE :
85JCAE "Prehled Prumyslove Toxikologie; Organicke Latky," Marhold, J.,
Prague, Czechoslovakia, Avicenum, 1986 Volume(issue)/page/year: -,285,1986
** ACUTE TOXICITY DATA **
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 2076 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
IJEBA6 Indian Journal of Experimental Biology. (Publications & Information
Directorate, CSIR, Hillside Rd., New Delhi 110 012, India) V.1- 1963-
Volume(issue)/page/year: 24,371,1986
TYPE OF TEST : LCLo - Lowest published lethal concentration
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 2000 ppm/4H
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
JIHTAB Journal of Industrial Hygiene and Toxicology. (Cambridge, MA)
V.18-31, 1936-49. For publisher information, see AEHLAU.
Volume(issue)/page/year: 31,343,1949
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 800 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
38MKAJ "Patty's Industrial Hygiene and Toxicology," 3rd rev. ed., Clayton,
G.D., and F.E. Clayton, eds., New York, John Wiley & Sons, Inc., 1978-82.
Vol. 3 originally pub. in 1979; pub. as 2nd rev. ed. in 1985.
Volume(issue)/page/year: 2C,4777,1982
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 2386 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
JAPMA8 Journal of the American Pharmaceutical Association, Scientific
Edition. (Washington, DC) V.29-49, 1940-60. For publisher information, see
JPMSAE. Volume(issue)/page/year: 45,669,1956
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 1600 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
38MKAJ "Patty's Industrial Hygiene and Toxicology," 3rd rev. ed., Clayton,
G.D., and F.E. Clayton, eds., New York, John Wiley & Sons, Inc., 1978-82.
Vol. 3 originally pub. in 1979; pub. as 2nd rev. ed. in 1985.
Volume(issue)/page/year: 2C,4777,1982
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Administration onto the skin
SPECIES OBSERVED : Rodent - guinea pig
DOSE/DURATION : 6600 uL/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
JIHTAB Journal of Industrial Hygiene and Toxicology. (Cambridge, MA)
V.18-31, 1936-49. For publisher information, see AEHLAU.
Volume(issue)/page/year: 26,269,1944
** OTHER MULTIPLE DOSE TOXICITY DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 38 gm/kg/15W-C
TOXIC EFFECTS :
Peripheral Nerve and Sensation - structural change in nerve or sheath
Behavioral - muscle weakness
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
135,58,1995
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 28 gm/kg/4W-C
TOXIC EFFECTS :
Behavioral - muscle weakness
Biochemical - Metabolism (Intermediary) - other proteins
Related to Chronic Data - changes in testicular weight
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
88,370,1987
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 23520 mg/kg/6W-C
TOXIC EFFECTS :
Peripheral Nerve and Sensation - structural change in nerve or sheath
Biochemical - Metabolism (Intermediary) - lipids including transport
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
54,210,1980
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 15982 mg/kg/46D-C
TOXIC EFFECTS :
Peripheral Nerve and Sensation - recording from peripheral motor nerve
REFERENCE :
TOLED5 Toxicology Letters. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1977- Volume(issue)/page/year: 23,141,1984
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 16800 mg/kg/8W-C
TOXIC EFFECTS :
Spinal Cord - other degenerative changes
Autonomic Nervous System - other (direct) parasympathomimetic
Biochemical - Metabolism (Intermediary) - other proteins
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
78,235,1985
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 21 gm/kg/21W-C
TOXIC EFFECTS :
Peripheral Nerve and Sensation - recording from afferent nerve
Peripheral Nerve and Sensation - recording from peripheral motor nerve
Nutritional and Gross Metabolic - weight loss or decreased weight gain
REFERENCE :
TOLED5 Toxicology Letters. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1977- Volume(issue)/page/year: 30,27,1986
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 9800 mg/kg/35D-I
TOXIC EFFECTS :
Peripheral Nerve and Sensation - recording from afferent nerve
Peripheral Nerve and Sensation - recording from peripheral motor nerve
Nutritional and Gross Metabolic - weight loss or decreased weight gain
REFERENCE :
JTEHD6 Journal of Toxicology and Environmental Health. (Hemisphere Pub.,
1025 Vermont Ave., NW, Washington, DC 20005) V.1- 1975/76-
Volume(issue)/page/year: 13,835,1984
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 24 gm/kg/12W-I
TOXIC EFFECTS :
Autonomic Nervous System - other (direct) parasympathomimetic
Behavioral - muscle weakness
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
84,36,1986
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 18551 mg/kg/13W-I
TOXIC EFFECTS :
Spinal Cord - other degenerative changes
Peripheral Nerve and Sensation - sensory change involving peripheral nerve
Peripheral Nerve and Sensation - recording from peripheral motor nerve
REFERENCE :
ENVRAL Environmental Research. (Academic Press, Inc., 1 E. First St.,
Duluth, MN 55802) V.1- 1967- Volume(issue)/page/year: 63,229,1993
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 19200 mg/kg/32D-I
TOXIC EFFECTS :
Peripheral Nerve and Sensation - recording from peripheral motor nerve
Nutritional and Gross Metabolic - weight loss or decreased weight gain
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
84,250,1986
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 16 mL/kg/20D-I
TOXIC EFFECTS :
Peripheral Nerve and Sensation - structural change in nerve or sheath
REFERENCE :
PHTOEH Pharmacology and Toxicology (Copenhagen). (Munksgaard International
Pub., POB 2148, DK-1016 Copenhagen K, Denmark) V.60- 1987-
Volume(issue)/page/year: 72,236,1993
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 16 gm/kg/8W-I
TOXIC EFFECTS :
Peripheral Nerve and Sensation - spastic paralysis with or without sensory
change
Peripheral Nerve and Sensation - structural change in nerve or sheath
Nutritional and Gross Metabolic - weight loss or decreased weight gain
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
139,15,1996
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 6300 mg/kg/28D-I
TOXIC EFFECTS :
Behavioral - somnolence (general depressed activity)
Behavioral - ataxia
Nutritional and Gross Metabolic - weight loss or decreased weight gain
REFERENCE :
NETEEC Neurotoxicology and Teratology. (Pergamon Press Inc., Maxwell House,
Fairview Park, Elmsford, NY 10523) V.9- 1987- Volume(issue)/page/year:
14,273,1992
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 5250 mg/kg/3W-I
TOXIC EFFECTS :
Brain and Coverings - recordings from specific areas of CNS
Nutritional and Gross Metabolic - weight loss or decreased weight gain
REFERENCE :
NETEEC Neurotoxicology and Teratology. (Pergamon Press Inc., Maxwell House,
Fairview Park, Elmsford, NY 10523) V.9- 1987- Volume(issue)/page/year:
12,623,1990
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Primate - monkey
DOSE/DURATION : 6210 mg/kg/17W-I
TOXIC EFFECTS :
Spinal Cord - other degenerative changes
Peripheral Nerve and Sensation - structural change in nerve or sheath
Behavioral - muscle weakness
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
98,166,1989
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Mammal - cat
DOSE/DURATION : 1440 mg/kg/6W-I
TOXIC EFFECTS :
Behavioral - ataxia
Behavioral - changes in psychophysiological tests
Peripheral Nerve and Sensation - recording from peripheral motor nerve
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
104,47,1990
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - guinea pig
DOSE/DURATION : 94517 mg/kg/24W-C
TOXIC EFFECTS :
Sense Organs and Special Senses (Eye) - miosis (pupillary constriction)
Nutritional and Gross Metabolic - weight loss or decreased weight gain
Related to Chronic Data - death
REFERENCE :
AIHAAP American Industrial Hygiene Association Journal. (AIHA, 475 Wolf
Ledges Pkwy., Akron, OH 44311) V.19- 1958- Volume(issue)/page/year:
39,94,1978
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Bird - chicken
DOSE/DURATION : 1575 mg/kg/3W-C
TOXIC EFFECTS :
Behavioral - somnolence (general depressed activity)
Behavioral - ataxia
Related to Chronic Data - death
REFERENCE :
TOXID9 Toxicologist. (Soc. of Toxicology, Inc., 475 Wolf Ledge Parkway,
Akron, OH 44311) V.1- 1981- Volume(issue)/page/year: 12,282,1992
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Bird - chicken
DOSE/DURATION : 9 gm/kg/90D-I
TOXIC EFFECTS :
Spinal Cord - other degenerative changes
Behavioral - ataxia
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
62,369,1982
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Bird - chicken
DOSE/DURATION : 9 gm/kg/90D-I
TOXIC EFFECTS :
Spinal Cord - demyelination
Spinal Cord - other degenerative changes
Peripheral Nerve and Sensation - spastic paralysis with or without sensory
change
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
62,369,1982
** REPRODUCTIVE DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE : 4200 mg/kg
SEX/DURATION : male 42 day(s) pre-mating
TOXIC EFFECTS :
Reproductive - Paternal Effects - testes, epididymis, sperm duct
Reproductive - Paternal Effects - other effects on male
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
62,262,1982
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE : 28 gm/kg
SEX/DURATION : male 28 day(s) pre-mating
TOXIC EFFECTS :
Reproductive - Paternal Effects - spermatogenesis (incl. genetic material,
sperm morphology, motility, and count)
REFERENCE :
EXMPA6 Experimental and Molecular Pathology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1962- Volume(issue)/page/year:
38,149,1983
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE : 28 gm/kg
SEX/DURATION : male 28 day(s) pre-mating
TOXIC EFFECTS :
Reproductive - Paternal Effects - testes, epididymis, sperm duct
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
88,370,1987
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE : 14934 mg/kg
SEX/DURATION : male 3 week(s) pre-mating
TOXIC EFFECTS :
Reproductive - Paternal Effects - testes, epididymis, sperm duct
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
94,76,1988
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE : 3400 mg/kg
SEX/DURATION : female 12-16 day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death,
e.g., stunted fetus)
REFERENCE :
TOLED5 Toxicology Letters. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1977- Volume(issue)/page/year: 59,59,1991
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE : 93 gm/kg
SEX/DURATION : female 6 week(s) pre-mating
TOXIC EFFECTS :
Reproductive - Maternal Effects - oogenesis
REFERENCE :
TXCYAC Toxicology. (Elsevier Scientific Pub. Ireland, Ltd., POB 85,
Limerick, Ireland) V.1- 1973- Volume(issue)/page/year: 76,197,1992
** MUTATION DATA **
TYPE OF TEST : Sex chromosome loss and nondisjunction
TEST SYSTEM : Yeast - Saccharomyces cerevisiae
DOSE/DURATION : 105 mmol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 341,83,1994
*** REVIEWS ***
TOXICOLOGY REVIEW
ARPTDI Annual Review of Pharmacology and Toxicology. (Annual Reviews, Inc.,
POB 10139, Palo Alto, CA 94303) V.16- 1976- Volume(issue)/page/year:
22,145,1982
*** STATUS IN U.S. ***
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JUNE 1998
*** END OF RECORD ***