*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : AY3600000
CHEMICAL NAME : Alanine, 3-(p-(bis(2-chloroethyl)amino)phenyl)-, DL-
CAS REGISTRY NUMBER : 531-76-0
OTHER CAS REGISTRY NOS. : 51-87-6
LAST UPDATED : 199604
DATA ITEMS CITED : 19
MOLECULAR FORMULA : C13-H18-Cl2-N2-O2
MOLECULAR WEIGHT : 305.23
WISWESSER LINE NOTATION : QVYZ1R DN2G2G -DL
COMPOUND DESCRIPTOR : Tumorigen
Drug
Mutagen
Reproductive Effector
SYNONYMS/TRADE NAMES :
* DL-3-(p-(Bis(2-chloroethyl)amino)phenyl)alanine
* 4-(Bis(2-chloroethyl)amino)-DL-phenylalanine
* CB-3307
* p-Di-(2-chloraethyl)-amino-DL-phenyl-alanin
* p-Di(2-chloroethyl)amino-DL-phenylalanine
* Merfalan
* Merphalan
* O-Merphalan
* NCI-CO4944
* NSC-14210
* DL-Phenylalanine, 4-(bis(2-chloroethyl)amino)-
* DL-Phenylalanine mustard
* Phenylalanin-lost
* dl-Phenylalanin-lost
* Sarcoclorin
* DL-Sarcolysin
* DL-Sarcolysine
*** HEALTH HAZARD DATA ***
** ACUTE TOXICITY DATA **
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 105 mg/kg
TOXIC EFFECTS :
Gastrointestinal - hypermotility, diarrhea
Nutritional and Gross Metabolic - weight loss or decreased weight gain
Tumorigenic - active as anti-cancer agent
REFERENCE :
AICCA6 Acta Unio Internationalis Contra Cancrum. (Louvain, Belgium) V.1-20,
1936-64. For publisher information, see IJCNAW. Volume(issue)/page/year:
20,144,1964
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 18 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
DKBSAS Doklady Biological Sciences (English Translation). Translation of
DANKAS: Biological Section. (Plenum Pub. Corp., 233 Spring St., New York, NY
10013) V.112- 1957- Volume(issue)/page/year: 171,801,1966
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 25 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
ARZNAD Arzneimittel-Forschung. Drug Research. (Editio Cantor Verlag,
Postfach 1255, W-7960 Aulendorf, Fed. Rep. Ger.) V.1- 1951-
Volume(issue)/page/year: 8,340,1958
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intracerebral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 250 ug/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
JPPMAB Journal of Pharmacy and Pharmacology. (Pharmaceutical Soc. of Great
Britain, 1 Lambeth High St., London SEI 7JN, UK) V.1- 1949-
Volume(issue)/page/year: 18,760,1966
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 26 mg/kg
TOXIC EFFECTS :
Tumorigenic - active as anti-cancer agent
REFERENCE :
ARZNAD Arzneimittel-Forschung. Drug Research. (Editio Cantor Verlag,
Postfach 1255, W-7960 Aulendorf, Fed. Rep. Ger.) V.1- 1951-
Volume(issue)/page/year: 16,634,1966
** TUMORIGENIC DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 98 mg/kg/26W-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Lungs, Thorax, or Respiration - tumors
Blood - lymphoma, including Hodgkin's disease
REFERENCE :
CANCAR Cancer (Philadelphia). (Lippincott/Harper, Journal Fulfillment
Dept., 2350 Virginia Ave., Hagerstown, MD 21740) V.1- 1948-
Volume(issue)/page/year: 40,1935,1977
** REPRODUCTIVE DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE : 8 mg/kg
SEX/DURATION : female 6 day(s) after conception
TOXIC EFFECTS :
Reproductive - Specific Developmental Abnormalities - Central Nervous System
Reproductive - Specific Developmental Abnormalities - eye/ear
Reproductive - Specific Developmental Abnormalities - craniofacial
(including nose and tongue)
REFERENCE :
DKBSAS Doklady Biological Sciences (English Translation). Translation of
DANKAS: Biological Section. (Plenum Pub. Corp., 233 Spring St., New York, NY
10013) V.112- 1957- Volume(issue)/page/year: 171,801,1966
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE : 8 mg/kg
SEX/DURATION : female 9 day(s) after conception
TOXIC EFFECTS :
Reproductive - Specific Developmental Abnormalities - musculoskeletal system
REFERENCE :
DKBSAS Doklady Biological Sciences (English Translation). Translation of
DANKAS: Biological Section. (Plenum Pub. Corp., 233 Spring St., New York, NY
10013) V.112- 1957- Volume(issue)/page/year: 171,801,1966
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE : 8 mg/kg
SEX/DURATION : female 4 day(s) after conception
TOXIC EFFECTS :
Reproductive - Fertility - post-implantation mortality (e.g. dead and/or
resorbed implants per total number of implants)
REFERENCE :
DKBSAS Doklady Biological Sciences (English Translation). Translation of
DANKAS: Biological Section. (Plenum Pub. Corp., 233 Spring St., New York, NY
10013) V.112- 1957- Volume(issue)/page/year: 171,801,1966
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE : 5 mg/kg
SEX/DURATION : female 6 day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Embryo or Fetus - fetal death
REFERENCE :
DKBSAS Doklady Biological Sciences (English Translation). Translation of
DANKAS: Biological Section. (Plenum Pub. Corp., 233 Spring St., New York, NY
10013) V.112- 1957- Volume(issue)/page/year: 171,801,1966
** MUTATION DATA **
TYPE OF TEST : Phage inhibition capacity
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 200 mg/L
REFERENCE :
ARMKA7 Archiv fuer Mikrobiologie. (Berlin, Fed. Rep. Ger.) V.1-94,
1930-73. For publisher information, see AMICCW. Volume(issue)/page/year:
51,9,1965
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Microorganism - not otherwise specified
DOSE/DURATION : 10 mmol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 23,5,1974
*** REVIEWS ***
IARC Cancer Review:Animal Sufficient Evidence
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 9,167,1975
IARC Cancer Review:Human No Adequate Data
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 9,167,1975
IARC Cancer Review:Group 2B
IMSUDL IARC Monographs, Supplement. (WHO Publications Centre USA, 49
Sheridan Ave., Albany, NY 12210) No.1- 1979- Volume(issue)/page/year:
7,56,1987
*** STATUS IN U.S. ***
EPA GENETOX PROGRAM 1988, Positive: Carcinogenicity-mouse/rat
EPA GENETOX PROGRAM 1988, Positive: D melanogaster Sex-linked lethal
NCI Carcinogenesis Studies (ipr);clear evidence:mouse
CANCAR Cancer (Philadelphia). (Lippincott/Harper, Journal Fulfillment
Dept., 2350 Virginia Ave., Hagerstown, MD 21740) V.1- 1948-
Volume(issue)/page/year: 40,1935,77
NCI Carcinogenesis Studies (ipr);no evidence:rat
CANCAR Cancer (Philadelphia). (Lippincott/Harper, Journal Fulfillment
Dept., 2350 Virginia Ave., Hagerstown, MD 21740) V.1- 1948-
Volume(issue)/page/year: 40,1935,77
*** END OF RECORD ***