*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : NQ9450000
CHEMICAL NAME : Isocyanic acid, methyl ester
CAS REGISTRY NUMBER : 624-83-9
BEILSTEIN REFERENCE NO. : 0605318
REFERENCE : 4-04-00-00247 (Beilstein Handbook Reference)
LAST UPDATED : 199806
DATA ITEMS CITED : 59
MOLECULAR FORMULA : C2-H3-N-O
MOLECULAR WEIGHT : 57.06
WISWESSER LINE NOTATION : OCN1
COMPOUND DESCRIPTOR : Mutagen
Reproductive Effector
Human
Primary Irritant
SYNONYMS/TRADE NAMES :
* Isocyanate de methyle
* Iso-cyanatomethane
* Methylisocyanaat
* Methyl isocyanat
* Methyl isocyanate
* Methylisokyanat
* Metil isocianato
* RCRA waste number P064
* TL 1450
*** HEALTH HAZARD DATA ***
** SKIN/EYE IRRITATION DATA **
TYPE OF TEST : Standard Draize test
ROUTE OF EXPOSURE : Administration onto the skin
SPECIES OBSERVED : Rodent - rabbit
DOSE/DURATION : 10 uL/24H
REACTION SEVERITY : Moderate
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: OTS0529151
TYPE OF TEST : Standard Draize test
ROUTE OF EXPOSURE : Administration into the eye
SPECIES OBSERVED : Rodent - rabbit
DOSE/DURATION : 5 uL/24H
REACTION SEVERITY : Severe
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: OTS0529151
** ACUTE TOXICITY DATA **
TYPE OF TEST : TCLo - Lowest published toxic concentration
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Human
DOSE/DURATION : 2 ppm
TOXIC EFFECTS :
Sense Organs and Special Senses (Olfaction) - effect, not otherwise
specified
Sense Organs and Special Senses (Eye) - conjunctive irritation
Lungs, Thorax, or Respiration - other changes
REFERENCE :
ATXKA8 Archiv fuer Toxikologie. (Berlin, Fed. Rep. Ger.) V.15-31, 1954-74.
For publisher information, see ARTODN. Volume(issue)/page/year:
20,235,1964
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 51500 ug/kg
TOXIC EFFECTS :
Peripheral Nerve and Sensation - flaccid paralysis without anesthesia
(usually neuromuscular blockage)
Behavioral - somnolence (general depressed activity)
Lungs, Thorax, or Respiration - respiratory depression
REFERENCE :
IJEBA6 Indian Journal of Experimental Biology. (Publications & Information
Directorate, CSIR, Hillside Rd., New Delhi 110 012, India) V.1- 1963-
Volume(issue)/page/year: 25,531,1987
TYPE OF TEST : LC50 - Lethal concentration, 50 percent kill
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 6100 ppb/6H
TOXIC EFFECTS :
Sense Organs and Special Senses (Eye) - lacrimation
Lungs, Thorax, or Respiration - dyspnea
REFERENCE :
FAATDF Fundamental and Applied Toxicology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1981- Volume(issue)/page/year:
6,747,1986
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Administration onto the skin
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 2780 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
YKYUA6 Yakkyoku. Pharmacy. (Nanzando, 4-1-11, Yushima, Bunkyo-ku, Tokyo,
Japan) V.1- 1950- Volume(issue)/page/year: 38,391,1987
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 261 mg/kg
TOXIC EFFECTS :
Peripheral Nerve and Sensation - flaccid paralysis without anesthesia
(usually neuromuscular blockage)
Behavioral - somnolence (general depressed activity)
Lungs, Thorax, or Respiration - respiratory depression
REFERENCE :
IJEBA6 Indian Journal of Experimental Biology. (Publications & Information
Directorate, CSIR, Hillside Rd., New Delhi 110 012, India) V.1- 1963-
Volume(issue)/page/year: 25,531,1987
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 120 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
42,417,1977
TYPE OF TEST : LC50 - Lethal concentration, 50 percent kill
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 12200 ppb/6H
TOXIC EFFECTS :
Sense Organs and Special Senses (Eye) - lacrimation
Lungs, Thorax, or Respiration - dyspnea
REFERENCE :
FAATDF Fundamental and Applied Toxicology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1981- Volume(issue)/page/year:
6,747,1986
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Administration onto the skin
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 1820 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
YKYUA6 Yakkyoku. Pharmacy. (Nanzando, 4-1-11, Yushima, Bunkyo-ku, Tokyo,
Japan) V.1- 1950- Volume(issue)/page/year: 38,391,1987
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 81900 ug/kg
TOXIC EFFECTS :
Peripheral Nerve and Sensation - flaccid paralysis without anesthesia
(usually neuromuscular blockage)
Behavioral - somnolence (general depressed activity)
Lungs, Thorax, or Respiration - respiratory depression
REFERENCE :
IJEBA6 Indian Journal of Experimental Biology. (Publications & Information
Directorate, CSIR, Hillside Rd., New Delhi 110 012, India) V.1- 1963-
Volume(issue)/page/year: 25,531,1987
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Administration onto the skin
SPECIES OBSERVED : Rodent - rabbit
DOSE/DURATION : 220 uL/kg
TOXIC EFFECTS :
Skin and Appendages - dermatitis, other (after systemic exposure)
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: OTS0529151
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rabbit
DOSE/DURATION : 126 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
TXCYAC Toxicology. (Elsevier Scientific Pub. Ireland, Ltd., POB 85,
Limerick, Ireland) V.1- 1973- Volume(issue)/page/year: 51,223,1988
TYPE OF TEST : LC50 - Lethal concentration, 50 percent kill
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - guinea pig
DOSE/DURATION : 5400 ppb/6H
TOXIC EFFECTS :
Sense Organs and Special Senses (Eye) - lacrimation
Lungs, Thorax, or Respiration - dyspnea
REFERENCE :
FAATDF Fundamental and Applied Toxicology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1981- Volume(issue)/page/year:
6,747,1986
** OTHER MULTIPLE DOSE TOXICITY DATA **
TYPE OF TEST : TCLo - Lowest published toxic concentration
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 3 ppm/6H/4D-I
TOXIC EFFECTS :
Lungs, Thorax, or Respiration - fibrosis, focal (pneumoconiosis)
Endocrine - other changes
Nutritional and Gross Metabolic - weight loss or decreased weight gain
REFERENCE :
FAATDF Fundamental and Applied Toxicology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1981- Volume(issue)/page/year:
9,480,1987
TYPE OF TEST : TCLo - Lowest published toxic concentration
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 3 ppm/6H/8D-I
TOXIC EFFECTS :
Lungs, Thorax, or Respiration - changes in lung weight
Blood - pigmented or nucleated red blood cells
Nutritional and Gross Metabolic - weight loss or decreased weight gain
REFERENCE :
EVHPAZ EHP, Environmental Health Perspectives. (U.S. Government Printing
Office, Supt of Documents, Washington, DC 20402) No.1- 1972-
Volume(issue)/page/year: 72,117,1987
TYPE OF TEST : TCLo - Lowest published toxic concentration
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 3 ppm/6H/4D-I
TOXIC EFFECTS :
Sense Organs and Special Senses (Olfaction) - effect, not otherwise
specified
Lungs, Thorax, or Respiration - structural or functional change in trachea
or bronchi
Related to Chronic Data - death
REFERENCE :
FAATDF Fundamental and Applied Toxicology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1981- Volume(issue)/page/year:
9,480,1987
** REPRODUCTIVE DATA **
TYPE OF TEST : TCLo - Lowest published toxic concentration
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - rat
DOSE : 9 ppm/3H
SEX/DURATION : female 10 day(s) after conception
TOXIC EFFECTS :
Reproductive - Fertility - post-implantation mortality (e.g. dead and/or
resorbed implants per total number of implants)
Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death,
e.g., stunted fetus)
REFERENCE :
JTEHD6 Journal of Toxicology and Environmental Health. (Hemisphere Pub.,
1025 Vermont Ave., NW, Washington, DC 20005) V.1- 1975/76-
Volume(issue)/page/year: 30,1,1990
TYPE OF TEST : TCLo - Lowest published toxic concentration
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - mouse
DOSE : 9 ppm/3H
SEX/DURATION : female 8 day(s) after conception
TOXIC EFFECTS :
Reproductive - Fertility - post-implantation mortality (e.g. dead and/or
resorbed implants per total number of implants)
Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death,
e.g., stunted fetus)
REFERENCE :
JTEHD6 Journal of Toxicology and Environmental Health. (Hemisphere Pub.,
1025 Vermont Ave., NW, Washington, DC 20005) V.1- 1975/76-
Volume(issue)/page/year: 30,1,1990
TYPE OF TEST : TCLo - Lowest published toxic concentration
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - mouse
DOSE : 9 ppm/3H
SEX/DURATION : female 8 day(s) after conception
TOXIC EFFECTS :
Reproductive - Fertility - litter size (e.g. # fetuses per litter; measured
before birth)
Reproductive - Effects on Embryo or Fetus - extra-embryonic structures
(e.g., placenta, umbilical cord)
Reproductive - Specific Developmental Abnormalities - musculoskeletal system
REFERENCE :
JTEHD6 Journal of Toxicology and Environmental Health. (Hemisphere Pub.,
1025 Vermont Ave., NW, Washington, DC 20005) V.1- 1975/76-
Volume(issue)/page/year: 21,265,1987
TYPE OF TEST : TCLo - Lowest published toxic concentration
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - mouse
DOSE : 9 ppm/3H
SEX/DURATION : female 1 day(s) after conception
TOXIC EFFECTS :
Reproductive - Paternal Effects - other effects on male
Reproductive - Fertility - mating performance (e.g. # sperm positive females
per # females mated; # copulations per # estrus cycles)
REFERENCE :
JTEHD6 Journal of Toxicology and Environmental Health. (Hemisphere Pub.,
1025 Vermont Ave., NW, Washington, DC 20005) V.1- 1975/76-
Volume(issue)/page/year: 21,265,1987
TYPE OF TEST : TCLo - Lowest published toxic concentration
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - mouse
DOSE : 1 ppm/6H
SEX/DURATION : female 14-17 day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Newborn - stillbirth
REFERENCE :
EVHPAZ EHP, Environmental Health Perspectives. (U.S. Government Printing
Office, Supt of Documents, Washington, DC 20402) No.1- 1972-
Volume(issue)/page/year: 72,149,1987
TYPE OF TEST : TCLo - Lowest published toxic concentration
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - mouse
DOSE : 3 ppm/6H
SEX/DURATION : female 14-17 day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Newborn - viability index (e.g., # alive at day 4
per # born alive)
Reproductive - Effects on Newborn - weaning or lactation index (e.g., #
alive at weaning per # alive at day 4)
REFERENCE :
EVHPAZ EHP, Environmental Health Perspectives. (U.S. Government Printing
Office, Supt of Documents, Washington, DC 20402) No.1- 1972-
Volume(issue)/page/year: 72,149,1987
** MUTATION DATA **
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 50 ug/plate
REFERENCE :
IJEBA6 Indian Journal of Experimental Biology. (Publications & Information
Directorate, CSIR, Hillside Rd., New Delhi 110 012, India) V.1- 1963-
Volume(issue)/page/year: 25,548,1987
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 100 ug/plate
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 204,123,1988
TYPE OF TEST : Mutation test systems - not otherwise specified
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 1 umol/plate
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 283,97,1992
TYPE OF TEST : Micronucleus test
ROUTE OF EXPOSURE : Inhalation
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 6 ppm/6H/4D (Continuous)
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 9,37,1987
TYPE OF TEST : Mutation test systems - not otherwise specified
ROUTE OF EXPOSURE : Inhalation
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 3 ppm/6H/4D (Continuous)
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 9,29,1987
TYPE OF TEST : Cytogenetic analysis
ROUTE OF EXPOSURE : Inhalation
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 3 ppm/6H/4D (Continuous)
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 9,37,1987
TYPE OF TEST : Sister chromatid exchange
ROUTE OF EXPOSURE : Inhalation
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 1 ppm/6H/4D (Continuous)
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 9,29,1987
TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Rodent - mouse Lymphocyte
DOSE/DURATION : 8 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 174,285,1986
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Ovary
DOSE/DURATION : 10100 ug/L
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 9,19,1987
TYPE OF TEST : Sister chromatid exchange
TEST SYSTEM : Rodent - hamster Ovary
DOSE/DURATION : 900 ug/L
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 9,19,1987
*** REVIEWS ***
ACGIH TLV-TWA 0.047 mg/m3 (0.02 ppm) (skin)
DTLVS* The Threshold Limit Values (TLVs) and Biological Exposure Indices
(BEIs) booklet issues by American Conference of Governmental Industrial
Hygienists (ACGIH), Cincinnati, OH, 1996 Volume(issue)/page/year:
TLV/BEI,1997
*** U.S. STANDARDS AND REGULATIONS ***
MSHA STANDARD-air:TWA 0.02 ppm (0.05 mg/m3) (skin)
DTLVS* The Threshold Limit Values (TLVs) and Biological Exposure Indices
(BEIs) booklet issues by American Conference of Governmental Industrial
Hygienists (ACGIH), Cincinnati, OH, 1996 Volume(issue)/page/year:
3,167,1971
OSHA PEL (Gen Indu):8H TWA 0.02 ppm (0.05 mg/m3) (skin)
CFRGBR Code of Federal Regulations. (U.S. Government Printing Office, Supt.
of Documents, Washington, DC 20402) Volume(issue)/page/year:
29,1910.1000,1994
OSHA PEL (Construc):8H TWA 0.02 ppm (0.05 mg/m3) (skin)
CFRGBR Code of Federal Regulations. (U.S. Government Printing Office, Supt.
of Documents, Washington, DC 20402) Volume(issue)/page/year:
29,1926.55,1994
OSHA PEL (Shipyard):8H TWA 0.02 ppm (0.05 mg/m3) (skin)
CFRGBR Code of Federal Regulations. (U.S. Government Printing Office, Supt.
of Documents, Washington, DC 20402) Volume(issue)/page/year:
29,1915.1000,1993
OSHA PEL (Fed Cont):8H TWA 0.02 ppm (0.05 mg/m3) (skin)
CFRGBR Code of Federal Regulations. (U.S. Government Printing Office, Supt.
of Documents, Washington, DC 20402) Volume(issue)/page/year:
41,50-204.50,1994
*** OCCUPATIONAL EXPOSURE LIMITS ***
OEL-BELGIUM:TWA 0.02 ppm (0.047 mg/m3);Skin JAN 1993
OEL-DENMARK:STEL 0.01 ppm (0.03 mg/m3);Skin JAN 1993
OEL-FRANCE:TWA 0.02 ppm (0.05 mg/m3);Skin JAN 1993
OEL-GERMANY:TWA 0.01 ppm (0.025 mg/m3) JAN 1993
OEL-HUNGARY:TWA 0.05 mg/m3;STEL 0.06 mg/m3;Skin JAN 1993
OEL-THE NETHERLANDS:TWA 0.02 ppm (0.05 mg/m3);Skin JAN 1993
OEL-THE PHILIPPINES:TWA 0.02 ppm (0.05 mg/m3);Skin JAN 1993
OEL-RUSSIA:STEL 0.05 mg/m3;Skin JAN 1993
OEL-SWITZERLAND:TWA 0.01 ppm (0.025 mg/m3);STEL 0.02 ppm JAN 1993
OEL-UNITED KINGDOM:TWA 0.002 mg/m3;STEL 0.07 mg/m3;Skin JAN 1993
OEL IN BULGARIA, COLOMBIA, JORDAN, KOREA check ACGIH TLV
OEL IN NEW ZEALAND, SINGAPORE, VIETNAM check ACGIH TLV
*** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA ***
NIOSH RECOMMENDED EXPOSURE LEVEL (REL) :
NIOSH REL TO METHYL ISOCYANATE-air:10H TWA 0.02 ppm (Sk)
REFERENCE :
NIOSH* National Institute for Occupational Safety and Health, U.S. Dept. of
Health, Education, and Welfare, Reports and Memoranda.
Volume(issue)/page/year: DHHS #92-100,1992
NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA :
NOHS - National Occupational Hazard Survey (1974)
NOHS Hazard Code - 47625
No. of Facilities: 56 (estimated)
No. of Industries: 2
No. of Occupations: 3
No. of Employees: 2381 (estimated)
NOES - National Occupational Exposure Survey (1983)
NOES Hazard Code - 47625
No. of Facilities: 7 (estimated)
No. of Industries: 1
No. of Occupations: 1
No. of Employees: 1822 (estimated)
No. of Female Employees: 272 (estimated)
*** STATUS IN U.S. ***
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
On EPA IRIS database
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JUNE 1998
OSHA ANALYTICAL METHOD #54
*** END OF RECORD ***