*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : BO9000000
CHEMICAL NAME : Ammonium,
(4-(bis(p-(dimethylamino)phenyl)methylene)-2,5-cycloh
exadien-1-ylidene) dimethyl-, chloride
CAS REGISTRY NUMBER : 548-62-9
LAST UPDATED : 199710
DATA ITEMS CITED : 48
MOLECULAR FORMULA : C25-H30-N3.Cl
MOLECULAR WEIGHT : 408.03
WISWESSER LINE NOTATION : L6Y DYJ AUYR DN1&1&R DN1&1& DUK1&1 &Q &G
COMPOUND DESCRIPTOR : Tumorigen
Mutagen
Reproductive Effector
Primary Irritant
SYNONYMS/TRADE NAMES :
* Adergon
* Aizen Crystal Violet
* Aizen Crystal Violet Extra Pure
* Aniline Violet
* Aniline Violet Pyoktanine
* Atmonil
* Avermin
* Axuris
* Badil
* Basic Violet 3
* Basic Violet BN
* Bismuth Violet
* Blaues pyoktanin
* Brilliant Violet 5B
* Calcozine Violet C
* Calcozine Violet 6BN
* C.I. 42555
* C.I. Basic Violet 3
* Crystal Violet
* Crystal Violet 6B
* Crystal Violet O
* Crystal Violet 5BO
* Crystal Violet 6BO
* Crystal Violet 10B
* Crystal Violet AO
* Crystal Violet AON
* Crystal Violet BP
* Crystal Violet BPC
* Crystal Violet Chloride
* Crystal Violet Extra Pure
* Crystal Violet Extra Pure APN
* Crystal Violet Extra Pure APNX
* Crystal Violet FN
* Crystal Violet HL2
* Crystal Violet Pure DSC
* Crystal Violet Pure DSC Brilliant
* Crystal Violet SS
* Crystal Violet Technical
* Crystal Violet USP
* Gentersal
* Gentian Violet
* Gentianaviolett
* Gentiaverm
* Genticid
* Gentioletten
* Hectograph Violet SR
* Hecto Violet R
* Hexamethyl-p-rosaniline chloride
* Hexamethyl p-rosaniline hydrochloride
* Hexamethyl Violet
* Hidaco Brilliant Crystal Violet
* Hidaco Crystal Violet
* Kristall-violett
* Meroxyl
* Meroxylan
* Meroxylan-wander
* Meroxyl-wander
* Methylrosanilinchlorid
* Methylrosaniline chloride
* Methylrosanilinum chloratum
* Methyl Violet 5BNO
* Methyl Violet 5BO
* Methyl Violet 10B
* Methyl Violet 10BD
* Methyl Violet 10BK
* Methyl Violet 10BN
* Methyl Violet 10BNS
* Methyl Violet 10BO
* Methylviolett
* Mitsui Crystal Violet
* NCI-C55969
* Oxiuran
* Oxycolor
* Oxyozyl
* Paper Blue R
* Pararosaniline, N,N,N',N',N'',N''-hexamethyl-, chloride
* Plastoresin Violet 5BO
* Pyoktanin
* Pyoverm
* Vermicid
* Vianin
* Viocid
* 12416 Violet
* Violet 6BN
* Violet 5BO
* Violet CP
* Violet gencianova
* Violet krystalova
* Violet xxiii
* Violet zasadita 3
*** HEALTH HAZARD DATA ***
** SKIN/EYE IRRITATION DATA **
TYPE OF TEST : Standard Draize test
ROUTE OF EXPOSURE : Administration onto the skin
SPECIES OBSERVED : Human
DOSE/DURATION : 3 mg/3D (Intermittent)
REACTION SEVERITY : Mild
REFERENCE :
85DKA8 "Cutaneous Toxicity, Proceedings of the 3rd Conference, 1976," Drill,
V.A., and P. Lazar, eds., New York, Academic Press, Inc. 1977
Volume(issue)/page/year: -,127,1977
TYPE OF TEST : Standard Draize test
ROUTE OF EXPOSURE : Administration onto the skin
SPECIES OBSERVED : Human
DOSE/DURATION : 2 mg/2D (Intermittent)
REACTION SEVERITY : Mild
REFERENCE :
ADVEA4 Acta Dermato-Venereologica. (Almqvist & Wiksell, POB 45150, S-10430
Stockholm, Sweden) V.1- 1920- Volume(issue)/page/year: 52,55,1972
TYPE OF TEST : Standard Draize test
ROUTE OF EXPOSURE : Administration onto the skin
SPECIES OBSERVED : Rodent - guinea pig
DOSE/DURATION : 6 mg/3D (Intermittent)
REFERENCE :
ADVEA4 Acta Dermato-Venereologica. (Almqvist & Wiksell, POB 45150, S-10430
Stockholm, Sweden) V.1- 1920- Volume(issue)/page/year: 52,55,1972
** ACUTE TOXICITY DATA **
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 420 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
ARZNAD Arzneimittel-Forschung. Drug Research. (Editio Cantor Verlag,
Postfach 1255, W-7960 Aulendorf, Fed. Rep. Ger.) V.1- 1951-
Volume(issue)/page/year: 1,5,1951
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 8900 ug/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
ARZNAD Arzneimittel-Forschung. Drug Research. (Editio Cantor Verlag,
Postfach 1255, W-7960 Aulendorf, Fed. Rep. Ger.) V.1- 1951-
Volume(issue)/page/year: 1,5,1951
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 96 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
ARZNAD Arzneimittel-Forschung. Drug Research. (Editio Cantor Verlag,
Postfach 1255, W-7960 Aulendorf, Fed. Rep. Ger.) V.1- 1951-
Volume(issue)/page/year: 1,5,1951
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 5100 ug/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
ARZNAD Arzneimittel-Forschung. Drug Research. (Editio Cantor Verlag,
Postfach 1255, W-7960 Aulendorf, Fed. Rep. Ger.) V.1- 1951-
Volume(issue)/page/year: 1,5,1951
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 20 mg/kg
TOXIC EFFECTS :
Lungs, Thorax, or Respiration - acute pulmonary edema
REFERENCE :
PSEBAA Proceedings of the Society for Experimental Biology and Medicine.
(Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1903/04-
Volume(issue)/page/year: 31,825,1934
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Mammal - dog
DOSE/DURATION : 1 gm/kg
TOXIC EFFECTS :
Behavioral - food intake (animal)
Gastrointestinal - nausea or vomiting
Nutritional and Gross Metabolic - weight loss or decreased weight gain
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
22,1,1972
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Mammal - cat
DOSE/DURATION : 100 mg/kg
TOXIC EFFECTS :
Gastrointestinal - hypermotility, diarrhea
Gastrointestinal - nausea or vomiting
Nutritional and Gross Metabolic - weight loss or decreased weight gain
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
22,1,1972
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rabbit
DOSE/DURATION : 150 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
ARZNAD Arzneimittel-Forschung. Drug Research. (Editio Cantor Verlag,
Postfach 1255, W-7960 Aulendorf, Fed. Rep. Ger.) V.1- 1951-
Volume(issue)/page/year: 1,5,1951
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rabbit
DOSE/DURATION : 5 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
ARZNAD Arzneimittel-Forschung. Drug Research. (Editio Cantor Verlag,
Postfach 1255, W-7960 Aulendorf, Fed. Rep. Ger.) V.1- 1951-
Volume(issue)/page/year: 1,5,1951
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraduodenal
SPECIES OBSERVED : Rodent - rabbit
DOSE/DURATION : 160 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
ARZNAD Arzneimittel-Forschung. Drug Research. (Editio Cantor Verlag,
Postfach 1255, W-7960 Aulendorf, Fed. Rep. Ger.) V.1- 1951-
Volume(issue)/page/year: 1,5,1951
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - guinea pig
DOSE/DURATION : 100 mg/kg
TOXIC EFFECTS :
Behavioral - ataxia
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
22,1,1972
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - guinea pig
DOSE/DURATION : 10 mg/kg
TOXIC EFFECTS :
Lungs, Thorax, or Respiration - acute pulmonary edema
REFERENCE :
PSEBAA Proceedings of the Society for Experimental Biology and Medicine.
(Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1903/04-
Volume(issue)/page/year: 31,825,1934
** OTHER MULTIPLE DOSE TOXICITY DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 4 mg/kg/2D-I
TOXIC EFFECTS :
Gastrointestinal - peritonitis
Related to Chronic Data - death
REFERENCE :
ARZNAD Arzneimittel-Forschung. Drug Research. (Editio Cantor Verlag,
Postfach 1255, W-7960 Aulendorf, Fed. Rep. Ger.) V.1- 1951-
Volume(issue)/page/year: 1,5,1951
** TUMORIGENIC DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 10950 mg/kg/2Y-C
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Endocrine - thyroid tumors
REFERENCE :
FCTOD7 Food and Chemical Toxicology. (Pergamon Press Inc., Maxwell House,
Fairview Park, Elmsford, NY 10523) V.20- 1982- Volume(issue)/page/year:
27,239,1989
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 25750 mg/kg/2Y-C
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Kidney, Ureter, Bladder - tumors
Reproductive - Tumorigenic effects - ovarian tumors
REFERENCE :
FAATDF Fundamental and Applied Toxicology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1981- Volume(issue)/page/year:
5,902,1985
** REPRODUCTIVE DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE : 100 mg/kg
SEX/DURATION : female 6-15 day(s) after conception
TOXIC EFFECTS :
Reproductive - Specific Developmental Abnormalities - musculoskeletal system
Reproductive - Specific Developmental Abnormalities - urogenital system
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: PB83-155754
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE : 40 mg/kg
SEX/DURATION : female 6-15 day(s) after conception
TOXIC EFFECTS :
Reproductive - Fertility - post-implantation mortality (e.g. dead and/or
resorbed implants per total number of implants)
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: PB83-155754
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE : 100 mg/kg
SEX/DURATION : female 6-15 day(s) after conception
TOXIC EFFECTS :
Reproductive - Specific Developmental Abnormalities - other developmental
abnormalities
REFERENCE :
TJADAB Teratology, The International Journal of Abnormal Development. (Alan
R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.1- 1968-
Volume(issue)/page/year: 33,64C,1986
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rabbit
DOSE : 7 mg/kg
SEX/DURATION : female 6-19 day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death,
e.g., stunted fetus)
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: PB83-182519
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rabbit
DOSE : 7 mg/kg
SEX/DURATION : female 6-19 day(s) after conception
TOXIC EFFECTS :
Reproductive - Fertility - post-implantation mortality (e.g. dead and/or
resorbed implants per total number of implants)
Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death,
e.g., stunted fetus)
REFERENCE :
TJADAB Teratology, The International Journal of Abnormal Development. (Alan
R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.1- 1968-
Volume(issue)/page/year: 33,64C,1986
** MUTATION DATA **
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 100 ng/plate
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 89,21,1981
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 500 ng/plate
REFERENCE :
TCMUD8 Teratogenesis, Carcinogenesis, and Mutagenesis. (Alan R. Liss, Inc.,
41 E. 11th St., New York, NY 10003) V.1- 1980- Volume(issue)/page/year:
10,449,1990
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 50 ug/plate
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 140,165,1984
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 25 ug/plate
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 140,165,1984
TYPE OF TEST : DNA repair
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 100 ng/well
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 3,429,1981
TYPE OF TEST : DNA adduct
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 10 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 89,95,1981
TYPE OF TEST : DNA repair
TEST SYSTEM : Bacteria - Bacillus subtilis
DOSE/DURATION : 2 mg/disc
REFERENCE :
TRENAF Kenkyu Nenpo--Tokyo-toritsu Eisei Kenkyusho. Annual Report of Tokyo
Metropolitan Research Laboratory of Public Health. (Tokyo-toritsu Eisei
Kenkyusho, 24-1, 3-chome, Hyakunin-cho, Shin-juku-ku, Tokyo 160, Japan) V.1-
1949/50- Volume(issue)/page/year: 27,153,1976
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Microorganism - not otherwise specified
DOSE/DURATION : 1 ppm
REFERENCE :
POASAD Proceedings of the Oklahoma Academy of Science. (Oklahoma Academy of
Science, c/o Executive Secretary-Treasurer, Southwestern Oklahoma State
Univ., Weatherford, OK 73096) V.1- 1910/1920- Volume(issue)/page/year:
34,114,1953
TYPE OF TEST : DNA inhibition
TEST SYSTEM : Human HeLa cell
DOSE/DURATION : 10 umol/L
REFERENCE :
RAREAE Radiation Research. (Academic Press, Inc., 1 E. First St., Duluth,
MN 55802) V.1- 1954- Volume(issue)/page/year: 37,334,1969
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Human HeLa cell
DOSE/DURATION : 500 ug/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 58,269,1978
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Human Lymphocyte
DOSE/DURATION : 500 ug/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 58,269,1978
TYPE OF TEST : DNA inhibition
TEST SYSTEM : Rodent - rat Liver
DOSE/DURATION : 10 nmol/L
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 8,99,1986
TYPE OF TEST : DNA damage
TEST SYSTEM : Rodent - mouse Lymphocyte
DOSE/DURATION : 1 mg/kg
REFERENCE :
TCMUD8 Teratogenesis, Carcinogenesis, and Mutagenesis. (Alan R. Liss, Inc.,
41 E. 11th St., New York, NY 10003) V.1- 1980- Volume(issue)/page/year:
10,449,1990
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Ovary
DOSE/DURATION : 500 ug/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 58,269,1978
TYPE OF TEST : DNA adduct
TEST SYSTEM : Mammal - species unspecified Lymphocyte
DOSE/DURATION : 10 pph
REFERENCE :
BIPMAA Biopolymers. (John Wiley & Sons, Inc., 605 Third Ave., New York, NY
10158) V.1- 1963- Volume(issue)/page/year: 11,2537,1972
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Mammal - species unspecified Cells - not otherwise
specified
DOSE/DURATION : 500 ug/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 58,269,1978
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Non-mammalian species Cells - not otherwise specified
DOSE/DURATION : 500 ug/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 58,269,1978
*** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA ***
NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA :
NOHS - National Occupational Hazard Survey (1974)
NOHS Hazard Code - M1517
No. of Facilities: 9858 (estimated)
No. of Industries: 58
No. of Occupations: 52
No. of Employees: 79824 (estimated)
NOES - National Occupational Exposure Survey (1983)
NOES Hazard Code - M1517
No. of Facilities: 3280 (estimated)
No. of Industries: 21
No. of Occupations: 34
No. of Employees: 75632 (estimated)
No. of Female Employees: 49319 (estimated)
*** STATUS IN U.S. ***
EPA GENETOX PROGRAM 1988, Positive: B subtilis rec assay
EPA GENETOX PROGRAM 1988, Negative: S cerevisiae-homozygosis
EPA GENETOX PROGRAM 1988, Inconclusive: B subtilis rec assay; Histidine
reversion-Ames test
EPA GENETOX PROGRAM 1988, Inconclusive: D melanogaster Sex-linked lethal
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JUNE 1998
*** END OF RECORD ***