*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : QJ6440000
CHEMICAL NAME : 1,3-Naphthalenedisulfonic acid,
6,6'-((3,3'-dimethyl-4,4'-biphenylylene)bis(azo))bis
(4-amino-5-hydroxy-, tetrasodium salt
CAS REGISTRY NUMBER : 314-13-6
LAST UPDATED : 199710
DATA ITEMS CITED : 26
MOLECULAR FORMULA : C34-H24-N6-O14-S4.4Na
MOLECULAR WEIGHT : 960.84
WISWESSER LINE NOTATION : L66J BZ CSWO ESWO JQ INUNR B1 DR C1 DNUN- IL66J BZ
CSWO ESWO JQ &-NA- 4
COMPOUND DESCRIPTOR : Tumorigen
Drug
Mutagen
Reproductive Effector
SYNONYMS/TRADE NAMES :
* Azovan Blue
* 4,4'-Bis(1-amino-8-hydroxy-2,4-disulfo-7-naphthylazo)-3,3'-bitolyl,
tetrasodium salt
* 4,4'-Bis(7-(1-amino-8-hydroxy-2,4-disulfo)naphthylazo)-3,3'-bitolyl,
tetrasodium salt
* 4,4'-Bis(1-amino-8-hydroxy-2,4-disulpho-7-naphthylazo)-3,3'-bitolyl,
tetrasodium salt
* 4,4'-Bis(7-(1-amino-8-hydroxy-2,4-disulpho)naphthylazo)-3,3'-bitolyl,
tetrasodium salt
* Blekit evansa
* C.I. 23860
* C.I. Direct Blue 53
* C.I. Direct Blue 53, tetrasodium salt
* Diamine Sky Blue FF
* Diazobleu
* Diazol Pure Blue BF
* Direct Blue 53
* Dye Evans Blue
* Evablin
* Evans Blue
* Evans Blue Dye
* Evans blue, sodium salt
* Geigy-blau 536
* Geigy Blue 536, med
* Modr Evansova
* Modr Prima 53
* T 1824
*** HEALTH HAZARD DATA ***
** ACUTE TOXICITY DATA **
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 5 mL/kg
TOXIC EFFECTS :
Cardiac - other changes
Lungs, Thorax, or Respiration - acute pulmonary edema
REFERENCE :
ARSUAX Archives of Surgery (Chicago). (AMA, 535 N. Dearborn St., Chicago,
IL 60610) V.1-61, 1920-50; V.81- 1960- Volume(issue)/page/year:
48,17,1944
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 340 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
BBRCA9 Biochemical and Biophysical Research Communications. (Academic
Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1959-
Volume(issue)/page/year: 136,64,1986
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Mammal - dog
DOSE/DURATION : 3 mL/kg
TOXIC EFFECTS :
Gastrointestinal - hypermotility, diarrhea
Gastrointestinal - nausea or vomiting
Lungs, Thorax, or Respiration - acute pulmonary edema
REFERENCE :
ARSUAX Archives of Surgery (Chicago). (AMA, 535 N. Dearborn St., Chicago,
IL 60610) V.1-61, 1920-50; V.81- 1960- Volume(issue)/page/year:
48,17,1944
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Mammal - cat
DOSE/DURATION : 1 mL/kg
TOXIC EFFECTS :
Gastrointestinal - hypermotility, diarrhea
Liver - other changes
Kidney, Ureter, Bladder - changes in tubules (including acute renal failure,
acute tubular necrosis)
REFERENCE :
ARSUAX Archives of Surgery (Chicago). (AMA, 535 N. Dearborn St., Chicago,
IL 60610) V.1-61, 1920-50; V.81- 1960- Volume(issue)/page/year:
48,17,1944
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - rabbit
DOSE/DURATION : 1 mL/kg
TOXIC EFFECTS :
Cardiac - other changes
Lungs, Thorax, or Respiration - acute pulmonary edema
REFERENCE :
ARSUAX Archives of Surgery (Chicago). (AMA, 535 N. Dearborn St., Chicago,
IL 60610) V.1-61, 1920-50; V.81- 1960- Volume(issue)/page/year:
48,17,1944
** OTHER MULTIPLE DOSE TOXICITY DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 620 mg/kg/10D-I
TOXIC EFFECTS :
Related to Chronic Data - death
REFERENCE :
BBRCA9 Biochemical and Biophysical Research Communications. (Academic
Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1959-
Volume(issue)/page/year: 136,64,1986
** TUMORIGENIC DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 850 mg/kg/34W-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Liver - tumors
Blood - leukemia
REFERENCE :
APHGBP Acta Pathologica (Belgrade). (Belgrade Univerzitet, Institute de
Pathologie, Belgrade, Yugoslavia) V.2/3- 1938- Volume(issue)/page/year:
33,1,1953
** REPRODUCTIVE DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE : 70 mg/kg
SEX/DURATION : female 8 day(s) after conception
TOXIC EFFECTS :
Reproductive - Fertility - post-implantation mortality (e.g. dead and/or
resorbed implants per total number of implants)
Reproductive - Specific Developmental Abnormalities - Central Nervous System
Reproductive - Specific Developmental Abnormalities - eye/ear
REFERENCE :
PSEBAA Proceedings of the Society for Experimental Biology and Medicine.
(Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1903/04-
Volume(issue)/page/year: 127,215,1968
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE : 70 mg/kg
SEX/DURATION : female 8 day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death,
e.g., stunted fetus)
REFERENCE :
PSEBAA Proceedings of the Society for Experimental Biology and Medicine.
(Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1903/04-
Volume(issue)/page/year: 127,215,1968
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE : 140 mg/kg
SEX/DURATION : female 8 day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Embryo or Fetus - other effects to embryo
REFERENCE :
TJADAB Teratology, The International Journal of Abnormal Development. (Alan
R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.1- 1968-
Volume(issue)/page/year: 2,85,1969
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE : 150 mg/kg
SEX/DURATION : female 7-9 day(s) after conception
TOXIC EFFECTS :
Reproductive - Specific Developmental Abnormalities - Central Nervous System
REFERENCE :
ANREAK Anatomical Record. (Alan R. Liss, Inc., 41 E. 11th St., New York, NY
10003) V.1- 1906/08- Volume(issue)/page/year: 118,369,1954
** MUTATION DATA **
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 33 ug/plate
REFERENCE :
CRNGDP Carcinogenesis (London). (Oxford Univ. Press, Pinkhill House,
Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1980-
Volume(issue)/page/year: 3,21,1982
TYPE OF TEST : Phage inhibition capacity
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 100 mmol/L
REFERENCE :
MDMIAZ Medycyna Doswiadczalna i Mikrobiologia. For English translation, see
EXMMAV. (Ars Polona, POB 1001, 00-068 Warsaw 1, Poland) V.1- 1949-
Volume(issue)/page/year: 31,11,1979
TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Rodent - rat Liver
DOSE/DURATION : 100 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 136,255,1984
TYPE OF TEST : Unscheduled DNA synthesis
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Rodent - rat
DOSE/DURATION : 200 mg/kg
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 7,101,1985
TYPE OF TEST : DNA adduct
ROUTE OF EXPOSURE : Administration onto the skin
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 192 umol/kg
REFERENCE :
CRNGDP Carcinogenesis (London). (Oxford Univ. Press, Pinkhill House,
Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1980-
Volume(issue)/page/year: 5,231,1984
TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Rodent - mouse Lymphocyte
DOSE/DURATION : 1001 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 189,223,1987
TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Rodent - hamster Liver
DOSE/DURATION : 10 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 136,255,1984
*** REVIEWS ***
IARC Cancer Review:Animal Limited Evidence
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 8,151,1975
IARC Cancer Review:Human No Adequate Data
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 8,151,1975
IARC Cancer Review:Group 3
IMSUDL IARC Monographs, Supplement. (WHO Publications Centre USA, 49
Sheridan Ave., Albany, NY 12210) No.1- 1979- Volume(issue)/page/year:
7,56,1987
TOXICOLOGY REVIEW
ADTEAS Advances in Teratology. (New York, NY) V.1-5, 1966-72.
Discontinued. Volume(issue)/page/year: 1,131,1966
*** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA ***
NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA :
NOES - National Occupational Exposure Survey (1983)
NOES Hazard Code - T0844
No. of Facilities: 162 (estimated)
No. of Industries: 3
No. of Occupations: 6
No. of Employees: 5350 (estimated)
No. of Female Employees: 2829 (estimated)
*** STATUS IN U.S. ***
EPA GENETOX PROGRAM 1988, Inconclusive: Histidine reversion-Ames test
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JUNE 1998
*** END OF RECORD ***