*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : RA0805000
CHEMICAL NAME : Nitrous acid, isobutyl ester
CAS REGISTRY NUMBER : 542-56-3
BEILSTEIN REFERENCE NO. : 1699518
REFERENCE : 4-01-00-01595 (Beilstein Handbook Reference)
LAST UPDATED : 199709
DATA ITEMS CITED : 21
MOLECULAR FORMULA : C4-H9-N-O2
MOLECULAR WEIGHT : 103.14
WISWESSER LINE NOTATION : ONO1Y1&1
COMPOUND DESCRIPTOR : Tumorigen
Mutagen
Human
SYNONYMS/TRADE NAMES :
* Blackjack
* Isobutyl nitrite
* NCI-C61052
* Nitrous acid, 2-methylpropyl ester
*** HEALTH HAZARD DATA ***
** ACUTE TOXICITY DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Human - man
DOSE/DURATION : 120 mg/kg
TOXIC EFFECTS :
Blood - methemoglobinemia-carboxyhemoglobin
Vascular - BP lowering not characterized in autonomic section
Cardiac - change in rate
REFERENCE :
AIMEAS Annals of Internal Medicine. (American College of Physicians, 4200
Pine St., Philadelphia, PA 19104) V.1- 1927- Volume(issue)/page/year:
92,637,1980
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 410 mg/kg
TOXIC EFFECTS :
Behavioral - general anesthetic
REFERENCE :
FEPRA7 Federation Proceedings, Federation of American Societies for
Experimental Biology. (Bethesda, MD) V.1-46, 1942-87.
Volume(issue)/page/year: 41,1583,1982
TYPE OF TEST : LC50 - Lethal concentration, 50 percent kill
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 777 ppm/4H
TOXIC EFFECTS :
Behavioral - altered sleep time (including change in righting reflex)
Behavioral - excitement
Lungs, Thorax, or Respiration - cyanosis
REFERENCE :
FAATDF Fundamental and Applied Toxicology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1981- Volume(issue)/page/year:
8,101,1987
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 205 mg/kg
TOXIC EFFECTS :
Liver - other changes
Blood - methemoglobinemia-carboxyhemoglobin
REFERENCE :
RCSADO Research Communications in Substances Abuse. (PJD Pub. Ltd., P.O.Box
966, Westbury, NY 11590) V.1- 1980- Volume(issue)/page/year: 3,233,1982
TYPE OF TEST : LC50 - Lethal concentration, 50 percent kill
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 1033 ppm/1H
TOXIC EFFECTS :
Behavioral - changes in motor activity (specific assay)
Lungs, Thorax, or Respiration - cyanosis
Kidney, Ureter, Bladder - urine volume increased
REFERENCE :
FAATDF Fundamental and Applied Toxicology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1981- Volume(issue)/page/year:
1,448,1981
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 184 mg/kg
TOXIC EFFECTS :
Liver - other changes
REFERENCE :
RCOCB8 Research Communications in Chemical Pathology and Pharmacology. (PJD
Pub. Ltd., P.O. Box 966, Westbury, NY 11590) V.1- 1970-
Volume(issue)/page/year: 26,75,1979
** OTHER MULTIPLE DOSE TOXICITY DATA **
TYPE OF TEST : TCLo - Lowest published toxic concentration
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 600 ppm/6H/2W-I
TOXIC EFFECTS :
Lungs, Thorax, or Respiration - other changes
Liver - other changes
Related to Chronic Data - death
REFERENCE :
FAATDF Fundamental and Applied Toxicology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1981- Volume(issue)/page/year:
19,169,1992
TYPE OF TEST : TCLo - Lowest published toxic concentration
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 150 ppm/6H/13W-I
TOXIC EFFECTS :
Sense Organs and Special Senses (Olfaction) - effect, not otherwise
specified
Blood - methemoglobinemia-carboxyhemoglobin
Blood - changes in erythrocyte (RBC) count
REFERENCE :
FAATDF Fundamental and Applied Toxicology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1981- Volume(issue)/page/year:
19,169,1992
TYPE OF TEST : TCLo - Lowest published toxic concentration
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 400 ppm/7H/60D-I
TOXIC EFFECTS :
Liver - changes in liver weight
Kidney, Ureter, Bladder - changes in bladder weight
Endocrine - changes in spleen weight
REFERENCE :
JJATDK JAT, Journal of Applied Toxicology. (John Wiley & Sons Ltd., Baffins
Lane, Chichester, W. Sussex PO19 1UD, UK) V.1- 1981-
Volume(issue)/page/year: 5,134,1985
TYPE OF TEST : TCLo - Lowest published toxic concentration
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 800 ppm/6H/2W-I
TOXIC EFFECTS :
Lungs, Thorax, or Respiration - changes in lung weight
Nutritional and Gross Metabolic - weight loss or decreased weight gain
Related to Chronic Data - death
REFERENCE :
FAATDF Fundamental and Applied Toxicology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1981- Volume(issue)/page/year:
19,169,1992
TYPE OF TEST : TCLo - Lowest published toxic concentration
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 300 ppm/6H/14W-I
TOXIC EFFECTS :
Lungs, Thorax, or Respiration - structural or functional change in trachea
or bronchi
Blood - changes in erythrocyte (RBC) count
Blood - changes in leukocyte (WBC) count
REFERENCE :
FAATDF Fundamental and Applied Toxicology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1981- Volume(issue)/page/year:
19,169,1992
TYPE OF TEST : TCLo - Lowest published toxic concentration
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 300 ppm/6.5H/18W-I
TOXIC EFFECTS :
Liver - changes in liver weight
Blood - methemoglobinemia-carboxyhemoglobin
Blood - changes in leukocyte (WBC) count
REFERENCE :
JTEHD6 Journal of Toxicology and Environmental Health. (Hemisphere Pub.,
1025 Vermont Ave., NW, Washington, DC 20005) V.1- 1975/76-
Volume(issue)/page/year: 15,823,1985
** TUMORIGENIC DATA **
TYPE OF TEST : TCLo - Lowest published toxic concentration
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 150 ppm/6H/2Y-I
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Lungs, Thorax, or Respiration - tumors
REFERENCE :
NTPTR* National Toxicology Program Technical Report Series. (Research
Triangle Park, NC 27709) No.206- Volume(issue)/page/year: NTP-TR-448,1996
TYPE OF TEST : TCLo - Lowest published toxic concentration
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 150 ppm/6H/2Y-I
TOXIC EFFECTS :
Tumorigenic - neoplastic by RTECS criteria
Lungs, Thorax, or Respiration - tumors
REFERENCE :
NTPTR* National Toxicology Program Technical Report Series. (Research
Triangle Park, NC 27709) No.206- Volume(issue)/page/year: NTP-TR-448,1996
** MUTATION DATA **
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 1 mg/plate
REFERENCE :
BSIBAC Bolletino della Societe Italiana di Biologia Sperimentale. (Casa
Editrice Idelson, Via A. de Gasperi, 55, 80133 Naples, Italy) V.2- 1927-
Volume(issue)/page/year: 56,816,1980
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 333 ug/plate
REFERENCE :
NTPTB* NTP Technical Bulletin. (National Toxicology Program, Landow Bldg.
3A-06, 7910 Woodmont Ave., Bethesda, MD 20205) Volume(issue)/page/year:
APR1982
TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Rodent - mouse Lymphocyte
DOSE/DURATION : 75900 nmol/L
REFERENCE :
EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E.
11th St., New York, NY 10003) V.10- 1987- Volume(issue)/page/year:
14,115,1989
*** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA ***
NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA :
NOES - National Occupational Exposure Survey (1983)
NOES Hazard Code - X4720
No. of Facilities: 13 (estimated)
No. of Industries: 1
No. of Occupations: 4
No. of Employees: 290 (estimated)
No. of Female Employees: 13 (estimated)
*** STATUS IN U.S. ***
EPA TSCA Section 8(b) CHEMICAL INVENTORY
NTP Carcinogenesis studies (inhalation);clear evidence:rat
NTPTR* National Toxicology Program Technical Report Series. (Research
Triangle Park, NC 27709) No.206- Volume(issue)/page/year: NTP-TR-448,96
NTP Carcinogenesis studies (inhalation);some evidence:mouse
NTPTR* National Toxicology Program Technical Report Series. (Research
Triangle Park, NC 27709) No.206- Volume(issue)/page/year: NTP-TR-448,96
*** END OF RECORD ***