*** CHEMICAL IDENTIFICATION *** RTECS NUMBER : SG1400000 CHEMICAL NAME : Phenazine, 3-amino-7-(dimethylamino)-2-methyl-, hydrochloride CAS REGISTRY NUMBER : 553-24-2 LAST UPDATED : 199701 DATA ITEMS CITED : 22 MOLECULAR FORMULA : C15-H16-N4.Cl-H MOLECULAR WEIGHT : 288.81 WISWESSER LINE NOTATION : T C666 BN INJ E1 FZ LN1&1 &GH COMPOUND DESCRIPTOR : Drug Mutagen Natural Product SYNONYMS/TRADE NAMES : * 3-Amino-7-dimethylamino-2-methylphenazine hydrochloride * Aminodimethylaminotoluaminozine hydrochloride * Cerven neutralni * Cerven toluylenova * Cerven zasadita 5 * C.I. 50040 * C.I. Basic Red 5 * C.I. Basic Red 5, monohydrochloride * Kernechtrot * Michrome No. 226 * Neutral Red * Neutral Red chloride * Neutral Red W * Nuclear Fast Red * Phenazine, 3-amino-7-(dimethylamino)-2-methyl-, monohydrochloride * Toluylene Red * N(sup 8),N(sup 8),3-Trimethyl-2,8-phenazinediamine monohydrochloride *** HEALTH HAZARD DATA *** ** ACUTE TOXICITY DATA ** TYPE OF TEST : LD50 - Lethal dose, 50 percent kill ROUTE OF EXPOSURE : Intravenous SPECIES OBSERVED : Rodent - rat DOSE/DURATION : 112 mg/kg TOXIC EFFECTS : Behavioral - convulsions or effect on seizure threshold Cardiac - other changes Lungs, Thorax, or Respiration - other changes REFERENCE : FEPRA7 Federation Proceedings, Federation of American Societies for Experimental Biology. (Bethesda, MD) V.1-46, 1942-87. Volume(issue)/page/year: 10,337,1951 TYPE OF TEST : LD50 - Lethal dose, 50 percent kill ROUTE OF EXPOSURE : Intraperitoneal SPECIES OBSERVED : Rodent - mouse DOSE/DURATION : 432 mg/kg TOXIC EFFECTS : Details of toxic effects not reported other than lethal dose value REFERENCE : ZYZAEU Zhongguo Yaoxue Zazhi. Chinese Pharmacuetical Journal. (China International Book Trading Corp., POB 2820, Beijing, Peop. China) V.24- 1989- Volume(issue)/page/year: 25,356,1990 TYPE OF TEST : LD50 - Lethal dose, 50 percent kill ROUTE OF EXPOSURE : Intravenous SPECIES OBSERVED : Rodent - mouse DOSE/DURATION : 142 mg/kg TOXIC EFFECTS : Behavioral - convulsions or effect on seizure threshold Cardiac - other changes Lungs, Thorax, or Respiration - other changes REFERENCE : FEPRA7 Federation Proceedings, Federation of American Societies for Experimental Biology. (Bethesda, MD) V.1-46, 1942-87. Volume(issue)/page/year: 10,337,1951 TYPE OF TEST : LD50 - Lethal dose, 50 percent kill ROUTE OF EXPOSURE : Intravenous SPECIES OBSERVED : Rodent - rabbit DOSE/DURATION : 96600 ug/kg TOXIC EFFECTS : Behavioral - convulsions or effect on seizure threshold Lungs, Thorax, or Respiration - respiratory stimulation Lungs, Thorax, or Respiration - other changes REFERENCE : SMBUA9 Stanford Medical Bulletin. (Palo Alto, CA) V.1-20, 1942-62. Discontinued. Volume(issue)/page/year: 9,96,1951 ** MUTATION DATA ** TYPE OF TEST : Mutation in microorganisms TEST SYSTEM : Bacteria - Salmonella typhimurium DOSE/DURATION : 25 ug/plate REFERENCE : AEMIDF Applied and Environmental Microbiology. (American Soc. for Microbiology, 1913 I St., NW, Washington, DC 20006) V.31- 1976- Volume(issue)/page/year: 42,641,1981 TYPE OF TEST : Mutation in microorganisms TEST SYSTEM : Bacteria - Escherichia coli DOSE/DURATION : 2500 ppt/3H REFERENCE : AMNTA4 American Naturalist. (Univ. of Chicago Press, Journals Div., POB 37005, Chicago, IL 60637) V.1- 1867- Volume(issue)/page/year: 85,119,1951 TYPE OF TEST : DNA repair TEST SYSTEM : Bacteria - Escherichia coli DOSE/DURATION : 2 ug/well REFERENCE : ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For publisher information, see EMMUEG. Volume(issue)/page/year: 3,429,1981 TYPE OF TEST : Mutation test systems - not otherwise specified TEST SYSTEM : Bacteria - Bacillus subtilis DOSE/DURATION : 144 mg/L REFERENCE : FOMIAZ Folia Microbiologica (Prague). (Academia, Vodickova 40, 112 29 Prague 1, Czechoslavakia) V.4- 1959- Volume(issue)/page/year: 22,329,1977 TYPE OF TEST : Sex chromosome loss and nondisjunction ROUTE OF EXPOSURE : Oral TEST SYSTEM : Insect - Drosophila melanogaster DOSE/DURATION : 1000 ppm REFERENCE : AMNTA4 American Naturalist. (Univ. of Chicago Press, Journals Div., POB 37005, Chicago, IL 60637) V.1- 1867- Volume(issue)/page/year: 87,295,1953 TYPE OF TEST : Sister chromatid exchange ROUTE OF EXPOSURE : Multiple routes TEST SYSTEM : Fish - not otherwise specified DOSE/DURATION : 1 ppm/4D (Continuous) REFERENCE : MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 64,205,1979 TYPE OF TEST : Cytogenetic analysis TEST SYSTEM : Human Lymphocyte DOSE/DURATION : 10 umol/L REFERENCE : MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 93,185,1982 TYPE OF TEST : DNA damage ROUTE OF EXPOSURE : Intraperitoneal TEST SYSTEM : Rodent - rat DOSE/DURATION : 96 mg/kg REFERENCE : CBINA8 Chemico-Biological Interactions. (Elsevier Scientific Pub. Ireland Ltd., POB 85, Limerick, Ireland) V.1- 1969- Volume(issue)/page/year: 22,287,1978 TYPE OF TEST : Cytogenetic analysis TEST SYSTEM : Rodent - hamster Ovary DOSE/DURATION : 2 umol/L/5H REFERENCE : MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 45,233,1977 TYPE OF TEST : Cytogenetic analysis ROUTE OF EXPOSURE : Parenteral TEST SYSTEM : Bird - chicken DOSE/DURATION : 85 ug/kg REFERENCE : 47JMAE "Cytogenetic Assays of Environmental Mutagens," Hsu, T.C., ed., Totowa, NJ, Allanheld, Osmun & Co., 1982 Volume(issue)/page/year: -,137,1982 TYPE OF TEST : Sister chromatid exchange ROUTE OF EXPOSURE : Parenteral TEST SYSTEM : Bird - chicken DOSE/DURATION : 85 ug/kg REFERENCE : 47JMAE "Cytogenetic Assays of Environmental Mutagens," Hsu, T.C., ed., Totowa, NJ, Allanheld, Osmun & Co., 1982 Volume(issue)/page/year: -,137,1982 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - M1598 No. of Facilities: 420 (estimated) No. of Industries: 5 No. of Occupations: 5 No. of Employees: 694 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - M1598 No. of Facilities: 746 (estimated) No. of Industries: 13 No. of Occupations: 12 No. of Employees: 23740 (estimated) No. of Female Employees: 19202 (estimated) *** STATUS IN U.S. *** EPA GENETOX PROGRAM 1988, Positive: Histidine reversion-Ames test EPA GENETOX PROGRAM 1988, Negative: N crassa-aneuploidy EPA GENETOX PROGRAM 1988, Inconclusive: E coli polA without S9 EPA GENETOX PROGRAM 1988, Inconclusive: D melanogaster Sex-linked lethal EPA TSCA Section 8(b) CHEMICAL INVENTORY *** END OF RECORD ***