*** CHEMICAL IDENTIFICATION ***

RTECS NUMBER            : SG1400000
CHEMICAL NAME           : Phenazine, 3-amino-7-(dimethylamino)-2-methyl-,
                          hydrochloride
CAS REGISTRY NUMBER     : 553-24-2
LAST UPDATED            : 199701
DATA ITEMS CITED        : 22
MOLECULAR FORMULA       : C15-H16-N4.Cl-H
MOLECULAR WEIGHT        : 288.81
WISWESSER LINE NOTATION : T C666 BN INJ E1 FZ LN1&1 &GH
COMPOUND DESCRIPTOR     : Drug
                          Mutagen
                          Natural Product
SYNONYMS/TRADE NAMES :
   * 3-Amino-7-dimethylamino-2-methylphenazine hydrochloride
   * Aminodimethylaminotoluaminozine hydrochloride
   * Cerven neutralni
   * Cerven toluylenova
   * Cerven zasadita 5
   * C.I. 50040
   * C.I. Basic Red 5
   * C.I. Basic Red 5, monohydrochloride
   * Kernechtrot
   * Michrome No. 226
   * Neutral Red
   * Neutral Red chloride
   * Neutral Red W
   * Nuclear Fast Red
   * Phenazine, 3-amino-7-(dimethylamino)-2-methyl-, monohydrochloride
   * Toluylene Red
   * N(sup 8),N(sup 8),3-Trimethyl-2,8-phenazinediamine monohydrochloride
 
                       *** HEALTH HAZARD DATA ***
 
                        ** ACUTE TOXICITY DATA **
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 112 mg/kg
TOXIC EFFECTS :
   Behavioral - convulsions or effect on seizure threshold
   Cardiac - other changes
   Lungs, Thorax, or Respiration - other changes
REFERENCE :
   FEPRA7 Federation Proceedings, Federation of American Societies for
   Experimental Biology. (Bethesda, MD)  V.1-46, 1942-87.
   Volume(issue)/page/year: 10,337,1951
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intraperitoneal
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 432 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   ZYZAEU Zhongguo Yaoxue Zazhi. Chinese Pharmacuetical Journal. (China
   International Book Trading Corp., POB 2820, Beijing, Peop. China) V.24-
   1989-  Volume(issue)/page/year: 25,356,1990
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 142 mg/kg
TOXIC EFFECTS :
   Behavioral - convulsions or effect on seizure threshold
   Cardiac - other changes
   Lungs, Thorax, or Respiration - other changes
REFERENCE :
   FEPRA7 Federation Proceedings, Federation of American Societies for
   Experimental Biology. (Bethesda, MD)  V.1-46, 1942-87.
   Volume(issue)/page/year: 10,337,1951
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Rodent - rabbit
DOSE/DURATION           : 96600 ug/kg
TOXIC EFFECTS :
   Behavioral - convulsions or effect on seizure threshold
   Lungs, Thorax, or Respiration - respiratory stimulation
   Lungs, Thorax, or Respiration - other changes
REFERENCE :
   SMBUA9 Stanford Medical Bulletin.  (Palo Alto, CA)  V.1-20, 1942-62.
   Discontinued.  Volume(issue)/page/year: 9,96,1951
 
                            ** MUTATION DATA **
 
TYPE OF TEST            : Mutation in microorganisms
TEST SYSTEM             : Bacteria - Salmonella typhimurium
DOSE/DURATION           : 25 ug/plate
REFERENCE :
   AEMIDF Applied and Environmental Microbiology.  (American Soc. for
   Microbiology, 1913 I St., NW, Washington, DC 20006)  V.31-    1976-
   Volume(issue)/page/year: 42,641,1981
 
TYPE OF TEST            : Mutation in microorganisms
TEST SYSTEM             : Bacteria - Escherichia coli
DOSE/DURATION           : 2500 ppt/3H
REFERENCE :
   AMNTA4 American Naturalist.  (Univ. of Chicago Press, Journals Div., POB
   37005, Chicago, IL 60637) V.1-    1867-  Volume(issue)/page/year:
   85,119,1951
 
TYPE OF TEST            : DNA repair
TEST SYSTEM             : Bacteria - Escherichia coli
DOSE/DURATION           : 2 ug/well
REFERENCE :
   ENMUDM Environmental Mutagenesis.  (New York, NY)  V.1-9, 1979-87.  For
   publisher information, see EMMUEG.  Volume(issue)/page/year: 3,429,1981
 
TYPE OF TEST            : Mutation test systems - not otherwise specified
TEST SYSTEM             : Bacteria - Bacillus subtilis
DOSE/DURATION           : 144 mg/L
REFERENCE :
   FOMIAZ Folia Microbiologica (Prague).  (Academia, Vodickova 40, 112 29
   Prague 1, Czechoslavakia) V.4-    1959-  Volume(issue)/page/year:
   22,329,1977
 
TYPE OF TEST            : Sex chromosome loss and nondisjunction
ROUTE OF EXPOSURE       : Oral
TEST SYSTEM             : Insect - Drosophila melanogaster
DOSE/DURATION           : 1000 ppm
REFERENCE :
   AMNTA4 American Naturalist.  (Univ. of Chicago Press, Journals Div., POB
   37005, Chicago, IL 60637) V.1-    1867-  Volume(issue)/page/year:
   87,295,1953
 
TYPE OF TEST            : Sister chromatid exchange
ROUTE OF EXPOSURE       : Multiple routes
TEST SYSTEM             : Fish - not otherwise specified
DOSE/DURATION           : 1 ppm/4D (Continuous)
REFERENCE :
   MUREAV Mutation Research.  (Elsevier Science Pub. B.V., POB 211, 1000 AE
   Amsterdam, Netherlands) V.1-    1964-  Volume(issue)/page/year: 64,205,1979
 
TYPE OF TEST            : Cytogenetic analysis
TEST SYSTEM             : Human Lymphocyte
DOSE/DURATION           : 10 umol/L
REFERENCE :
   MUREAV Mutation Research.  (Elsevier Science Pub. B.V., POB 211, 1000 AE
   Amsterdam, Netherlands) V.1-    1964-  Volume(issue)/page/year: 93,185,1982
 
TYPE OF TEST            : DNA damage
ROUTE OF EXPOSURE       : Intraperitoneal
TEST SYSTEM             : Rodent - rat
DOSE/DURATION           : 96 mg/kg
REFERENCE :
   CBINA8 Chemico-Biological Interactions.  (Elsevier Scientific Pub. Ireland
   Ltd., POB 85, Limerick, Ireland)  V.1-    1969-  Volume(issue)/page/year:
   22,287,1978
 
TYPE OF TEST            : Cytogenetic analysis
TEST SYSTEM             : Rodent - hamster Ovary
DOSE/DURATION           : 2 umol/L/5H
REFERENCE :
   MUREAV Mutation Research.  (Elsevier Science Pub. B.V., POB 211, 1000 AE
   Amsterdam, Netherlands) V.1-    1964-  Volume(issue)/page/year: 45,233,1977
 
TYPE OF TEST            : Cytogenetic analysis
ROUTE OF EXPOSURE       : Parenteral
TEST SYSTEM             : Bird - chicken
DOSE/DURATION           : 85 ug/kg
REFERENCE :
   47JMAE "Cytogenetic Assays of Environmental Mutagens,"  Hsu, T.C., ed.,
   Totowa, NJ, Allanheld, Osmun & Co., 1982  Volume(issue)/page/year:
   -,137,1982
 
TYPE OF TEST            : Sister chromatid exchange
ROUTE OF EXPOSURE       : Parenteral
TEST SYSTEM             : Bird - chicken
DOSE/DURATION           : 85 ug/kg
REFERENCE :
   47JMAE "Cytogenetic Assays of Environmental Mutagens,"  Hsu, T.C., ed.,
   Totowa, NJ, Allanheld, Osmun & Co., 1982  Volume(issue)/page/year:
   -,137,1982

         *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA ***

NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA :
   NOHS - National Occupational Hazard Survey (1974)
     NOHS Hazard Code - M1598
       No. of Facilities: 420 (estimated)
       No. of Industries: 5
       No. of Occupations: 5
       No. of Employees: 694 (estimated)
   
   NOES - National Occupational Exposure Survey (1983)
     NOES Hazard Code - M1598
       No. of Facilities: 746 (estimated)
       No. of Industries: 13
       No. of Occupations: 12
       No. of Employees: 23740 (estimated)
       No. of Female Employees: 19202 (estimated)

                          *** STATUS IN U.S. ***
 
   EPA GENETOX PROGRAM 1988, Positive: Histidine reversion-Ames test
   
   EPA GENETOX PROGRAM 1988, Negative: N crassa-aneuploidy
   
   EPA GENETOX PROGRAM 1988, Inconclusive: E coli polA without S9
   
   EPA GENETOX PROGRAM 1988, Inconclusive: D melanogaster Sex-linked lethal
   
   EPA TSCA Section 8(b) CHEMICAL INVENTORY

 
                            *** END OF RECORD ***