*** CHEMICAL IDENTIFICATION *** RTECS NUMBER : SM1140500 CHEMICAL NAME : Phenol, p-(5-(5-(4-methyl-1-piperazinyl)-2-benzimidazolyl)-2- benzimidazolyl)-, trihydrochloride CAS REGISTRY NUMBER : 23491-45-4 LAST UPDATED : 199709 DATA ITEMS CITED : 14 MOLECULAR FORMULA : C25-H24-N6-O.3Cl-H MOLECULAR WEIGHT : 533.93 WISWESSER LINE NOTATION : T56 BM DNJ CR DQ& G- CT56 BM DNJ G- AT6N DNTJ D1 &GH 3 COMPOUND DESCRIPTOR : Mutagen SYNONYMS/TRADE NAMES : * Bisbenzimidazole trihydrochloride * Bisbenzimide * H 33258 * HOE 33058 * HOE 33258 * 33258 Hoechst * Hoechst 33258 * Hoechst Dye 33258 * NSC-322921 * Phenol, 4-(5-(4-methyl-1-piperazinyl)(2,5'-bi-1H-benzimidazol)-2'-yl)-, trihydrochloride *** HEALTH HAZARD DATA *** ** ACUTE TOXICITY DATA ** TYPE OF TEST : LD50 - Lethal dose, 50 percent kill ROUTE OF EXPOSURE : Intravenous SPECIES OBSERVED : Rodent - rat DOSE/DURATION : 32200 ug/kg TOXIC EFFECTS : Behavioral - somnolence (general depressed activity) Lungs, Thorax, or Respiration - dyspnea Skin and Appendages - hair REFERENCE : NTIS** National Technical Information Service. (Springfield, VA 22161) Formerly U.S. Clearinghouse for Scientific & Technical Information. Volume(issue)/page/year: PB84-171206 TYPE OF TEST : LD50 - Lethal dose, 50 percent kill ROUTE OF EXPOSURE : Intravenous SPECIES OBSERVED : Rodent - mouse DOSE/DURATION : 36900 ug/kg TOXIC EFFECTS : Behavioral - somnolence (general depressed activity) Lungs, Thorax, or Respiration - dyspnea Skin and Appendages - hair REFERENCE : NTIS** National Technical Information Service. (Springfield, VA 22161) Formerly U.S. Clearinghouse for Scientific & Technical Information. Volume(issue)/page/year: PB84-171206 ** MUTATION DATA ** TYPE OF TEST : DNA adduct TEST SYSTEM : Bacteria - Escherichia coli DOSE/DURATION : 10 mg/L REFERENCE : MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 89,95,1981 TYPE OF TEST : DNA damage TEST SYSTEM : Microorganism - not otherwise specified DOSE/DURATION : 2 umol/L REFERENCE : CHROAU Chromosoma. (Springer-Verlag New York, Inc., Service Center, 44 Hartz Way, Secaucus, NJ 07094) V.1- 1939- Volume(issue)/page/year: 52,297,1975 TYPE OF TEST : DNA damage TEST SYSTEM : Microorganism - not otherwise specified DOSE/DURATION : 2 umol/L REFERENCE : CHROAU Chromosoma. (Springer-Verlag New York, Inc., Service Center, 44 Hartz Way, Secaucus, NJ 07094) V.1- 1939- Volume(issue)/page/year: 52,297,1975 TYPE OF TEST : Cytogenetic analysis TEST SYSTEM : Rodent - rat Fibroblast DOSE/DURATION : 1 mg/L REFERENCE : ZZACAG Zeitschrift fuer Zellforschung und Mikroskopische Anatomie. (Berlin, Fed. Rep. Ger.) V.1-147, 1924-73. For publisher information, see CTSRCS. Volume(issue)/page/year: 104,127,1970 TYPE OF TEST : Cytogenetic analysis TEST SYSTEM : Rodent - mouse Fibroblast DOSE/DURATION : 1 mg/L REFERENCE : ZZACAG Zeitschrift fuer Zellforschung und Mikroskopische Anatomie. (Berlin, Fed. Rep. Ger.) V.1-147, 1924-73. For publisher information, see CTSRCS. Volume(issue)/page/year: 104,127,1970 TYPE OF TEST : Cytogenetic analysis TEST SYSTEM : Rodent - hamster Cells - not otherwise specified DOSE/DURATION : 80 mg/L REFERENCE : CHROAU Chromosoma. (Springer-Verlag New York, Inc., Service Center, 44 Hartz Way, Secaucus, NJ 07094) V.1- 1939- Volume(issue)/page/year: 56,41,1976 TYPE OF TEST : Sister chromatid exchange TEST SYSTEM : Rodent - hamster Ovary DOSE/DURATION : 10 umol/L REFERENCE : NATUAS Nature. (Nature Subscription Dept., POB 1018, Manasguan, NJ 08736) V.1- 1869- Volume(issue)/page/year: 258,121,1975 TYPE OF TEST : Sister chromatid exchange TEST SYSTEM : Rodent - hamster Lung DOSE/DURATION : 15 umol/L REFERENCE : HEREAY Hereditas (Lund, Sweden). (Gjoerloffsgatan 121, S-261 34 Landskrona, Sweden) V.1- 1947- Volume(issue)/page/year: 96,295,1982 TYPE OF TEST : DNA damage TEST SYSTEM : Mammal - species unspecified Lymphocyte DOSE/DURATION : 1600 nmol/L REFERENCE : CHROAU Chromosoma. (Springer-Verlag New York, Inc., Service Center, 44 Hartz Way, Secaucus, NJ 07094) V.1- 1939- Volume(issue)/page/year: 52,297,1975 *** STATUS IN U.S. *** EPA GENETOX PROGRAM 1988, Positive/dose response: In vitro SCE-nonhuman EPA GENETOX PROGRAM 1988, Negative: In vivo SCE-nonhuman EPA TSCA Section 8(b) CHEMICAL INVENTORY *** END OF RECORD ***