*** CHEMICAL IDENTIFICATION ***

RTECS NUMBER            : SM1140500
CHEMICAL NAME           : Phenol,
                          p-(5-(5-(4-methyl-1-piperazinyl)-2-benzimidazolyl)-2-
                          benzimidazolyl)-, trihydrochloride
CAS REGISTRY NUMBER     : 23491-45-4
LAST UPDATED            : 199709
DATA ITEMS CITED        : 14
MOLECULAR FORMULA       : C25-H24-N6-O.3Cl-H
MOLECULAR WEIGHT        : 533.93
WISWESSER LINE NOTATION : T56 BM DNJ CR DQ& G- CT56 BM DNJ G- AT6N DNTJ D1 &GH
                          3
COMPOUND DESCRIPTOR     : Mutagen
SYNONYMS/TRADE NAMES :
   * Bisbenzimidazole trihydrochloride
   * Bisbenzimide
   * H 33258
   * HOE 33058
   * HOE 33258
   * 33258 Hoechst
   * Hoechst 33258
   * Hoechst Dye 33258
   * NSC-322921
   * Phenol, 4-(5-(4-methyl-1-piperazinyl)(2,5'-bi-1H-benzimidazol)-2'-yl)-,  
   trihydrochloride
 
                       *** HEALTH HAZARD DATA ***
 
                        ** ACUTE TOXICITY DATA **
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 32200 ug/kg
TOXIC EFFECTS :
   Behavioral - somnolence (general depressed activity)
   Lungs, Thorax, or Respiration - dyspnea
   Skin and Appendages - hair
REFERENCE :
   NTIS** National Technical Information Service.  (Springfield, VA 22161)
   Formerly U.S. Clearinghouse for Scientific & Technical Information.
   Volume(issue)/page/year: PB84-171206
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 36900 ug/kg
TOXIC EFFECTS :
   Behavioral - somnolence (general depressed activity)
   Lungs, Thorax, or Respiration - dyspnea
   Skin and Appendages - hair
REFERENCE :
   NTIS** National Technical Information Service.  (Springfield, VA 22161)
   Formerly U.S. Clearinghouse for Scientific & Technical Information.
   Volume(issue)/page/year: PB84-171206
 
                            ** MUTATION DATA **
 
TYPE OF TEST            : DNA adduct
TEST SYSTEM             : Bacteria - Escherichia coli
DOSE/DURATION           : 10 mg/L
REFERENCE :
   MUREAV Mutation Research.  (Elsevier Science Pub. B.V., POB 211, 1000 AE
   Amsterdam, Netherlands) V.1-    1964-  Volume(issue)/page/year: 89,95,1981
 
TYPE OF TEST            : DNA damage
TEST SYSTEM             : Microorganism - not otherwise specified
DOSE/DURATION           : 2 umol/L
REFERENCE :
   CHROAU Chromosoma.  (Springer-Verlag New York, Inc., Service Center, 44
   Hartz Way, Secaucus, NJ 07094)  V.1-    1939-  Volume(issue)/page/year:
   52,297,1975
 
TYPE OF TEST            : DNA damage
TEST SYSTEM             : Microorganism - not otherwise specified
DOSE/DURATION           : 2 umol/L
REFERENCE :
   CHROAU Chromosoma.  (Springer-Verlag New York, Inc., Service Center, 44
   Hartz Way, Secaucus, NJ 07094)  V.1-    1939-  Volume(issue)/page/year:
   52,297,1975
 
TYPE OF TEST            : Cytogenetic analysis
TEST SYSTEM             : Rodent - rat Fibroblast
DOSE/DURATION           : 1 mg/L
REFERENCE :
   ZZACAG Zeitschrift fuer Zellforschung und Mikroskopische Anatomie. (Berlin,
   Fed. Rep. Ger.) V.1-147, 1924-73.  For publisher information, see CTSRCS. 
   Volume(issue)/page/year: 104,127,1970
 
TYPE OF TEST            : Cytogenetic analysis
TEST SYSTEM             : Rodent - mouse Fibroblast
DOSE/DURATION           : 1 mg/L
REFERENCE :
   ZZACAG Zeitschrift fuer Zellforschung und Mikroskopische Anatomie. (Berlin,
   Fed. Rep. Ger.) V.1-147, 1924-73.  For publisher information, see CTSRCS. 
   Volume(issue)/page/year: 104,127,1970
 
TYPE OF TEST            : Cytogenetic analysis
TEST SYSTEM             : Rodent - hamster Cells - not otherwise specified
DOSE/DURATION           : 80 mg/L
REFERENCE :
   CHROAU Chromosoma.  (Springer-Verlag New York, Inc., Service Center, 44
   Hartz Way, Secaucus, NJ 07094)  V.1-    1939-  Volume(issue)/page/year:
   56,41,1976
 
TYPE OF TEST            : Sister chromatid exchange
TEST SYSTEM             : Rodent - hamster Ovary
DOSE/DURATION           : 10 umol/L
REFERENCE :
   NATUAS Nature.  (Nature Subscription Dept., POB 1018, Manasguan, NJ 08736)
   V.1-    1869-  Volume(issue)/page/year: 258,121,1975
 
TYPE OF TEST            : Sister chromatid exchange
TEST SYSTEM             : Rodent - hamster Lung
DOSE/DURATION           : 15 umol/L
REFERENCE :
   HEREAY Hereditas (Lund, Sweden).  (Gjoerloffsgatan 121, S-261 34 Landskrona,
   Sweden)  V.1-    1947-  Volume(issue)/page/year: 96,295,1982
 
TYPE OF TEST            : DNA damage
TEST SYSTEM             : Mammal - species unspecified Lymphocyte
DOSE/DURATION           : 1600 nmol/L
REFERENCE :
   CHROAU Chromosoma.  (Springer-Verlag New York, Inc., Service Center, 44
   Hartz Way, Secaucus, NJ 07094)  V.1-    1939-  Volume(issue)/page/year:
   52,297,1975

                          *** STATUS IN U.S. ***
 
   EPA GENETOX PROGRAM 1988, Positive/dose response: In vitro SCE-nonhuman
   
   EPA GENETOX PROGRAM 1988, Negative: In vivo SCE-nonhuman
   
   EPA TSCA Section 8(b) CHEMICAL INVENTORY

 
                            *** END OF RECORD ***