*** CHEMICAL IDENTIFICATION *** RTECS NUMBER : SO5600000 CHEMICAL NAME : 3H-Phenothiazine, 7-(dimethylamino)-3-(methylimino)-, 3-methochloride CAS REGISTRY NUMBER : 61-73-4 LAST UPDATED : 199710 DATA ITEMS CITED : 36 MOLECULAR FORMULA : C16-H18-N3-S.Cl MOLECULAR WEIGHT : 319.88 WISWESSER LINE NOTATION : T C666 BS EY INJ EUK1&1 MN1&1 &G &421 COMPOUND DESCRIPTOR : Drug Mutagen Reproductive Effector Human SYNONYMS/TRADE NAMES : * Aizen Methylene Blue BH * Aizen Methylene Blue FZ * Basic Blue 9 * 3,7-Bis (dimethylamino) phenazathionium chloride * 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride * Calcozine Blue ZF * Chromosmon * C.I. 52015 * C.I. Basic Blue 9 * D and C Blue No. 1 * Ext D and C Blue No. 1 * External Blue 1 * Hidaco Methylene Blue Salt Free * Leather Pure Blue HB * Methylenblau * Methylene Blue * Methylene Blue A * Methylene Blue B * Methylene Blue 2B * Methylene Blue BB * Methylene Blue BBA * Methylene Blue BB (zinc free) * Methylene Blue BD * Methylene Blue 2BF * Methylene Blue 2BN * Methylene Blue BP * Methylene Blue 2BP * Methylene Blue BPC * Methylene Blue BX * Methylene Blue BZ * Methylene Blue chloride * Methylene Blue chloride (biological stain) * Methylene Blue D * Methylene Blue FZ * Methylene Blue G * Methylene Blue GZ * Methylene Blue HGG * Methylene Blue IAD * Methylene Blue I (medicinal) * Methylene Blue JFA * Methylene Blue (medicinal) * Methylene Blue N * Methylene Blue NF (medicinal) * Methylene Blue NZ * Methylene Blue Polychrome * Methylene Blue SG * Methylene Blue SP * Methylene Blue USP (medicinal) * Methylene Blue USP XII (medicinal) * Methylene Blue ZF * Methylene Blue Zinc Free * Methylene Blue ZX * Methylenium ceruleum * Methylthionine * Methylthionine chloride * Methylthionium chloride * Mitsui Methylene Blue * Modr Methylenova * Modr Rozpoustedlova 8 * Modr Zasadita 9 * Phenothiazin-5-ium, 3,7-bis(dimethylamino)-, chloride * Sandocryl Blue BRL * Schultz No. 1038 * Swiss Blue * Tetramethylene Blue * Tetramethylthionine chloride * Yamamoto Methylene Blue B * Yamamoto Methylene Blue ZF *** HEALTH HAZARD DATA *** ** ACUTE TOXICITY DATA ** TYPE OF TEST : TDLo - Lowest published toxic dose ROUTE OF EXPOSURE : Unreported SPECIES OBSERVED : Human - infant DOSE/DURATION : 15 mg/kg TOXIC EFFECTS : Lungs, Thorax, or Respiration - cyanosis Blood - other changes REFERENCE : 34ZIAG "Toxicology of Drugs and Chemicals," Deichmann, W.B., New York, Academic Press, Inc., 1969 Volume(issue)/page/year: -,390,1969 TYPE OF TEST : LD50 - Lethal dose, 50 percent kill ROUTE OF EXPOSURE : Oral SPECIES OBSERVED : Rodent - rat DOSE/DURATION : 1180 mg/kg TOXIC EFFECTS : Details of toxic effects not reported other than lethal dose value REFERENCE : 85JCAE "Prehled Prumyslove Toxikologie; Organicke Latky," Marhold, J., Prague, Czechoslovakia, Avicenum, 1986 Volume(issue)/page/year: -,1334,1986 TYPE OF TEST : LD50 - Lethal dose, 50 percent kill ROUTE OF EXPOSURE : Intraperitoneal SPECIES OBSERVED : Rodent - rat DOSE/DURATION : 180 mg/kg TOXIC EFFECTS : Details of toxic effects not reported other than lethal dose value REFERENCE : AEPPAE Naunyn-Schmiedeberg's Archiv fuer Experimentelle Pathologie und Pharmakologie. (Berlin, Ger.) V.110-253, 1925-66. For publisher information, see NSAPCC. Volume(issue)/page/year: 204,288,1947 TYPE OF TEST : LD50 - Lethal dose, 50 percent kill ROUTE OF EXPOSURE : Subcutaneous SPECIES OBSERVED : Rodent - rat DOSE/DURATION : 190 mg/kg TOXIC EFFECTS : Details of toxic effects not reported other than lethal dose value REFERENCE : NIIRDN Drugs in Japan (Ethical Drugs). (Yakugyo Jiho Co., Ltd., Tokyo, Japan) Volume(issue)/page/year: -,1185,1990 TYPE OF TEST : LD50 - Lethal dose, 50 percent kill ROUTE OF EXPOSURE : Intravenous SPECIES OBSERVED : Rodent - rat DOSE/DURATION : 1250 mg/kg TOXIC EFFECTS : Details of toxic effects not reported other than lethal dose value REFERENCE : ARZNAD Arzneimittel-Forschung. Drug Research. (Editio Cantor Verlag, Postfach 1255, W-7960 Aulendorf, Fed. Rep. Ger.) V.1- 1951- Volume(issue)/page/year: 18,676,1968 TYPE OF TEST : LD50 - Lethal dose, 50 percent kill ROUTE OF EXPOSURE : Oral SPECIES OBSERVED : Rodent - mouse DOSE/DURATION : 3500 mg/kg TOXIC EFFECTS : Details of toxic effects not reported other than lethal dose value REFERENCE : CKFRAY Ceskoslovenska Farmacie. (PNS-Ustredni Expedice a Dovoz Tisku, Kafkova 19, 160 00 Prague 6, Czechoslovakia) V.1- 1952- Volume(issue)/page/year: 12,94,1963 TYPE OF TEST : LD50 - Lethal dose, 50 percent kill ROUTE OF EXPOSURE : Intraperitoneal SPECIES OBSERVED : Rodent - mouse DOSE/DURATION : 150 mg/kg TOXIC EFFECTS : Details of toxic effects not reported other than lethal dose value REFERENCE : NTIS** National Technical Information Service. (Springfield, VA 22161) Formerly U.S. Clearinghouse for Scientific & Technical Information. Volume(issue)/page/year: AD691-490 TYPE OF TEST : LD50 - Lethal dose, 50 percent kill ROUTE OF EXPOSURE : Intravenous SPECIES OBSERVED : Rodent - mouse DOSE/DURATION : 77 mg/kg TOXIC EFFECTS : Details of toxic effects not reported other than lethal dose value REFERENCE : CKFRAY Ceskoslovenska Farmacie. (PNS-Ustredni Expedice a Dovoz Tisku, Kafkova 19, 160 00 Prague 6, Czechoslovakia) V.1- 1952- Volume(issue)/page/year: 12,94,1963 TYPE OF TEST : LDLo - Lowest published lethal dose ROUTE OF EXPOSURE : Oral SPECIES OBSERVED : Mammal - dog DOSE/DURATION : 500 mg/kg TOXIC EFFECTS : Details of toxic effects not reported other than lethal dose value REFERENCE : HBAMAK "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." (Leipzig, Ger. Dem. Rep.) Volume(issue)/page/year: 4,1366,1935 TYPE OF TEST : LDLo - Lowest published lethal dose ROUTE OF EXPOSURE : Intravenous SPECIES OBSERVED : Mammal - dog DOSE/DURATION : 50 mg/kg TOXIC EFFECTS : Details of toxic effects not reported other than lethal dose value REFERENCE : HBAMAK "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." (Leipzig, Ger. Dem. Rep.) Volume(issue)/page/year: 4,1366,1935 TYPE OF TEST : LDLo - Lowest published lethal dose ROUTE OF EXPOSURE : Intravenous SPECIES OBSERVED : Primate - monkey DOSE/DURATION : 10 mg/kg TOXIC EFFECTS : Details of toxic effects not reported other than lethal dose value REFERENCE : HBAMAK "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." (Leipzig, Ger. Dem. Rep.) Volume(issue)/page/year: 4,1366,1935 TYPE OF TEST : LDLo - Lowest published lethal dose ROUTE OF EXPOSURE : Intravenous SPECIES OBSERVED : Mammal - cat DOSE/DURATION : 41 mg/kg TOXIC EFFECTS : Details of toxic effects not reported other than lethal dose value REFERENCE : AIMEAS Annals of Internal Medicine. (American College of Physicians, 4200 Pine St., Philadelphia, PA 19104) V.1- 1927- Volume(issue)/page/year: 7,738,1933 TYPE OF TEST : LDLo - Lowest published lethal dose ROUTE OF EXPOSURE : Oral SPECIES OBSERVED : Rodent - rabbit DOSE/DURATION : 1 gm/kg TOXIC EFFECTS : Details of toxic effects not reported other than lethal dose value REFERENCE : HBAMAK "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." (Leipzig, Ger. Dem. Rep.) Volume(issue)/page/year: 4,1366,1935 TYPE OF TEST : LDLo - Lowest published lethal dose ROUTE OF EXPOSURE : Subcutaneous SPECIES OBSERVED : Rodent - guinea pig DOSE/DURATION : 300 mg/kg TOXIC EFFECTS : Details of toxic effects not reported other than lethal dose value REFERENCE : HBAMAK "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." (Leipzig, Ger. Dem. Rep.) Volume(issue)/page/year: 4,1366,1935 TYPE OF TEST : LD50 - Lethal dose, 50 percent kill ROUTE OF EXPOSURE : Intravenous SPECIES OBSERVED : Mammal - domestic DOSE/DURATION : 42300 ug/kg TOXIC EFFECTS : Details of toxic effects not reported other than lethal dose value REFERENCE : JVPTD9 Journal of Veterinary Pharmacology and Therapeutics. (Blackwell Scientific Pub. Ltd., POB 88, Oxford, UK) V.1- 1978- Volume(issue)/page/year: 7,225,1984 ** REPRODUCTIVE DATA ** TYPE OF TEST : TDLo - Lowest published toxic dose ROUTE OF EXPOSURE : Oral SPECIES OBSERVED : Rodent - rat DOSE : 2500 mg/kg SEX/DURATION : female 1-22 day(s) after conception TOXIC EFFECTS : Reproductive - Fertility - post-implantation mortality (e.g. dead and/or resorbed implants per total number of implants) REFERENCE : AJANA2 American Journal of Anatomy. (Alan R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.1-192, 1901-91. Volume(issue)/page/year: 110,29,1962 ** MUTATION DATA ** TYPE OF TEST : Mutation in microorganisms TEST SYSTEM : Bacteria - Salmonella typhimurium DOSE/DURATION : 20 ug/plate REFERENCE : ABCHA6 Agricultural and Biological Chemistry. (Maruzen Co. Ltd., POB 5050, Tokyo International, Tokyo 100-31, Japan) V.25- 1961- Volume(issue)/page/year: 45,327,1981 TYPE OF TEST : DNA repair TEST SYSTEM : Bacteria - Salmonella typhimurium DOSE/DURATION : 10 pph REFERENCE : AGACBH Agents and Actions, A Swiss Journal of Pharmacology. (Birkhaeuser Verlag, POB 133, CH-4010 Basel, Switzerland) V.1- 1969/70- Volume(issue)/page/year: 4,286,1974 TYPE OF TEST : Mutation in microorganisms TEST SYSTEM : Bacteria - Escherichia coli DOSE/DURATION : 2 umol/L REFERENCE : MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 137,1,1984 TYPE OF TEST : DNA repair TEST SYSTEM : Bacteria - Escherichia coli DOSE/DURATION : 160 ug/well REFERENCE : ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For publisher information, see EMMUEG. Volume(issue)/page/year: 3,429,1981 TYPE OF TEST : DNA adduct TEST SYSTEM : Bacteria - Escherichia coli DOSE/DURATION : 70 umol/L REFERENCE : MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 89,95,1981 TYPE OF TEST : DNA repair TEST SYSTEM : Bacteria - Bacillus subtilis DOSE/DURATION : 2 mg/disc REFERENCE : TRENAF Kenkyu Nenpo--Tokyo-toritsu Eisei Kenkyusho. Annual Report of Tokyo Metropolitan Research Laboratory of Public Health. (Tokyo-toritsu Eisei Kenkyusho, 24-1, 3-chome, Hyakunin-cho, Shin-juku-ku, Tokyo 160, Japan) V.1- 1949/50- Volume(issue)/page/year: 27,153,1976 TYPE OF TEST : Mutation in microorganisms TEST SYSTEM : Microorganism - not otherwise specified DOSE/DURATION : 20 ppm REFERENCE : POASAD Proceedings of the Oklahoma Academy of Science. (Oklahoma Academy of Science, c/o Executive Secretary-Treasurer, Southwestern Oklahoma State Univ., Weatherford, OK 73096) V.1- 1910/1920- Volume(issue)/page/year: 34,114,1953 TYPE OF TEST : Gene conversion and mitotic recombination TEST SYSTEM : Yeast - Saccharomyces cerevisiae DOSE/DURATION : 10 pph REFERENCE : AGACBH Agents and Actions, A Swiss Journal of Pharmacology. (Birkhaeuser Verlag, POB 133, CH-4010 Basel, Switzerland) V.1- 1969/70- Volume(issue)/page/year: 4,286,1974 TYPE OF TEST : DNA adduct TEST SYSTEM : Rodent - mouse Ascites tumor DOSE/DURATION : 70 umol/L REFERENCE : MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 89,95,1981 TYPE OF TEST : DNA adduct TEST SYSTEM : Mammal - species unspecified Lymphocyte DOSE/DURATION : 1830 nmol/L REFERENCE : BIPMAA Biopolymers. (John Wiley & Sons, Inc., 605 Third Ave., New York, NY 10158) V.1- 1963- Volume(issue)/page/year: 12,1099,1973 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - M1469 No. of Facilities: 4471 (estimated) No. of Industries: 35 No. of Occupations: 34 No. of Employees: 25916 (estimated) NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - T1406 No. of Facilities: 150 (estimated) No. of Industries: 2 No. of Occupations: 2 No. of Employees: 664 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - M1469 No. of Facilities: 5956 (estimated) No. of Industries: 29 No. of Occupations: 51 No. of Employees: 74208 (estimated) No. of Female Employees: 43305 (estimated) *** STATUS IN U.S. *** EPA GENETOX PROGRAM 1988, Positive/dose response: In vitro SCE-nonhuman EPA GENETOX PROGRAM 1988, Negative: B subtilis rec assay; S cerevisiae gene conversion EPA GENETOX PROGRAM 1988, Inconclusive: B subtilis rec assay; Histidine reversion-Ames test EPA GENETOX PROGRAM 1988, Inconclusive: D melanogaster Sex-linked lethal EPA TSCA Section 8(b) CHEMICAL INVENTORY EPA TSCA Section 8(e) Risk Notification, 8EHQ-0892-8826 EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JUNE 1998 *** END OF RECORD ***