*** CHEMICAL IDENTIFICATION ***

RTECS NUMBER            : SO5600000
CHEMICAL NAME           : 3H-Phenothiazine, 7-(dimethylamino)-3-(methylimino)-,
                          3-methochloride
CAS REGISTRY NUMBER     : 61-73-4
LAST UPDATED            : 199710
DATA ITEMS CITED        : 36
MOLECULAR FORMULA       : C16-H18-N3-S.Cl
MOLECULAR WEIGHT        : 319.88
WISWESSER LINE NOTATION : T C666 BS EY INJ EUK1&1 MN1&1  &G &421
COMPOUND DESCRIPTOR     : Drug
                          Mutagen
                          Reproductive Effector
                          Human
SYNONYMS/TRADE NAMES :
   * Aizen Methylene Blue BH
   * Aizen Methylene Blue FZ
   * Basic Blue 9
   * 3,7-Bis (dimethylamino) phenazathionium chloride
   * 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride
   * Calcozine Blue ZF
   * Chromosmon
   * C.I. 52015
   * C.I. Basic Blue 9
   * D and C Blue No. 1
   * Ext D and C Blue No. 1
   * External Blue 1
   * Hidaco Methylene Blue Salt Free
   * Leather Pure Blue HB
   * Methylenblau
   * Methylene Blue
   * Methylene Blue A
   * Methylene Blue B
   * Methylene Blue 2B
   * Methylene Blue BB
   * Methylene Blue BBA
   * Methylene Blue BB (zinc free)
   * Methylene Blue BD
   * Methylene Blue 2BF
   * Methylene Blue 2BN
   * Methylene Blue BP
   * Methylene Blue 2BP
   * Methylene Blue BPC
   * Methylene Blue BX
   * Methylene Blue BZ
   * Methylene Blue chloride
   * Methylene Blue chloride (biological stain)
   * Methylene Blue D
   * Methylene Blue FZ
   * Methylene Blue G
   * Methylene Blue GZ
   * Methylene Blue HGG
   * Methylene Blue IAD
   * Methylene Blue I (medicinal)
   * Methylene Blue JFA
   * Methylene Blue (medicinal)
   * Methylene Blue N
   * Methylene Blue NF (medicinal)
   * Methylene Blue NZ
   * Methylene Blue Polychrome
   * Methylene Blue SG
   * Methylene Blue SP
   * Methylene Blue USP (medicinal)
   * Methylene Blue USP XII (medicinal)
   * Methylene Blue ZF
   * Methylene Blue Zinc Free
   * Methylene Blue ZX
   * Methylenium ceruleum
   * Methylthionine
   * Methylthionine chloride
   * Methylthionium chloride
   * Mitsui Methylene Blue
   * Modr Methylenova
   * Modr Rozpoustedlova 8
   * Modr Zasadita 9
   * Phenothiazin-5-ium, 3,7-bis(dimethylamino)-, chloride
   * Sandocryl Blue BRL
   * Schultz No. 1038
   * Swiss Blue
   * Tetramethylene Blue
   * Tetramethylthionine chloride
   * Yamamoto Methylene Blue B
   * Yamamoto Methylene Blue ZF
 
                       *** HEALTH HAZARD DATA ***
 
                        ** ACUTE TOXICITY DATA **
 
TYPE OF TEST            : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE       : Unreported
SPECIES OBSERVED        : Human - infant
DOSE/DURATION           : 15 mg/kg
TOXIC EFFECTS :
   Lungs, Thorax, or Respiration - cyanosis
   Blood - other changes
REFERENCE :
   34ZIAG "Toxicology of Drugs and Chemicals," Deichmann, W.B., New York,
   Academic Press, Inc., 1969  Volume(issue)/page/year: -,390,1969
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Oral
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 1180 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   85JCAE "Prehled Prumyslove Toxikologie; Organicke Latky," Marhold, J.,
   Prague, Czechoslovakia, Avicenum, 1986  Volume(issue)/page/year: -,1334,1986
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intraperitoneal
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 180 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   AEPPAE Naunyn-Schmiedeberg's Archiv fuer Experimentelle Pathologie und
   Pharmakologie.  (Berlin, Ger.) V.110-253, 1925-66.  For publisher
   information, see NSAPCC.  Volume(issue)/page/year: 204,288,1947
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Subcutaneous
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 190 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   NIIRDN Drugs in Japan (Ethical Drugs).  (Yakugyo Jiho Co., Ltd., Tokyo,
   Japan)  Volume(issue)/page/year: -,1185,1990
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 1250 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   ARZNAD Arzneimittel-Forschung. Drug Research.  (Editio Cantor Verlag,
   Postfach 1255, W-7960 Aulendorf, Fed. Rep. Ger.)  V.1-    1951-
   Volume(issue)/page/year: 18,676,1968
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Oral
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 3500 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   CKFRAY Ceskoslovenska Farmacie.  (PNS-Ustredni Expedice a Dovoz Tisku,
   Kafkova 19, 160 00 Prague 6, Czechoslovakia)  V.1-    1952-
   Volume(issue)/page/year: 12,94,1963
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intraperitoneal
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 150 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   NTIS** National Technical Information Service.  (Springfield, VA 22161)
   Formerly U.S. Clearinghouse for Scientific & Technical Information.
   Volume(issue)/page/year: AD691-490
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 77 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   CKFRAY Ceskoslovenska Farmacie.  (PNS-Ustredni Expedice a Dovoz Tisku,
   Kafkova 19, 160 00 Prague 6, Czechoslovakia)  V.1-    1952-
   Volume(issue)/page/year: 12,94,1963
 
TYPE OF TEST            : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE       : Oral
SPECIES OBSERVED        : Mammal - dog
DOSE/DURATION           : 500 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   HBAMAK "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." (Leipzig,
   Ger. Dem. Rep.)  Volume(issue)/page/year: 4,1366,1935
 
TYPE OF TEST            : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Mammal - dog
DOSE/DURATION           : 50 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   HBAMAK "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." (Leipzig,
   Ger. Dem. Rep.)  Volume(issue)/page/year: 4,1366,1935
 
TYPE OF TEST            : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Primate - monkey
DOSE/DURATION           : 10 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   HBAMAK "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." (Leipzig,
   Ger. Dem. Rep.)  Volume(issue)/page/year: 4,1366,1935
 
TYPE OF TEST            : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Mammal - cat
DOSE/DURATION           : 41 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   AIMEAS Annals of Internal Medicine.  (American College of Physicians, 4200
   Pine St., Philadelphia, PA 19104)  V.1-    1927-  Volume(issue)/page/year:
   7,738,1933
 
TYPE OF TEST            : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE       : Oral
SPECIES OBSERVED        : Rodent - rabbit
DOSE/DURATION           : 1 gm/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   HBAMAK "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." (Leipzig,
   Ger. Dem. Rep.)  Volume(issue)/page/year: 4,1366,1935
 
TYPE OF TEST            : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE       : Subcutaneous
SPECIES OBSERVED        : Rodent - guinea pig
DOSE/DURATION           : 300 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   HBAMAK "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." (Leipzig,
   Ger. Dem. Rep.)  Volume(issue)/page/year: 4,1366,1935
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Mammal - domestic
DOSE/DURATION           : 42300 ug/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   JVPTD9 Journal of Veterinary Pharmacology and Therapeutics.  (Blackwell
   Scientific Pub. Ltd., POB 88, Oxford, UK)  V.1-    1978-
   Volume(issue)/page/year: 7,225,1984
 
                          ** REPRODUCTIVE DATA **
 
TYPE OF TEST            : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE       : Oral
SPECIES OBSERVED        : Rodent - rat
DOSE                    : 2500 mg/kg
SEX/DURATION            : female 1-22 day(s) after conception
TOXIC EFFECTS :
   Reproductive - Fertility - post-implantation mortality (e.g. dead and/or
   resorbed implants per total number of implants)
REFERENCE :
   AJANA2 American Journal of Anatomy.  (Alan R. Liss, Inc., 41 E. 11th St.,
   New York, NY 10003) V.1-192, 1901-91.  Volume(issue)/page/year: 110,29,1962
 
                            ** MUTATION DATA **
 
TYPE OF TEST            : Mutation in microorganisms
TEST SYSTEM             : Bacteria - Salmonella typhimurium
DOSE/DURATION           : 20 ug/plate
REFERENCE :
   ABCHA6 Agricultural and Biological Chemistry.  (Maruzen Co. Ltd., POB 5050,
   Tokyo International, Tokyo 100-31, Japan)  V.25-    1961-
   Volume(issue)/page/year: 45,327,1981
 
TYPE OF TEST            : DNA repair
TEST SYSTEM             : Bacteria - Salmonella typhimurium
DOSE/DURATION           : 10 pph
REFERENCE :
   AGACBH Agents and Actions, A Swiss Journal of Pharmacology.  (Birkhaeuser
   Verlag, POB 133, CH-4010 Basel, Switzerland)  V.1-    1969/70-
   Volume(issue)/page/year: 4,286,1974
 
TYPE OF TEST            : Mutation in microorganisms
TEST SYSTEM             : Bacteria - Escherichia coli
DOSE/DURATION           : 2 umol/L
REFERENCE :
   MUREAV Mutation Research.  (Elsevier Science Pub. B.V., POB 211, 1000 AE
   Amsterdam, Netherlands) V.1-    1964-  Volume(issue)/page/year: 137,1,1984
 
TYPE OF TEST            : DNA repair
TEST SYSTEM             : Bacteria - Escherichia coli
DOSE/DURATION           : 160 ug/well
REFERENCE :
   ENMUDM Environmental Mutagenesis.  (New York, NY)  V.1-9, 1979-87.  For
   publisher information, see EMMUEG.  Volume(issue)/page/year: 3,429,1981
 
TYPE OF TEST            : DNA adduct
TEST SYSTEM             : Bacteria - Escherichia coli
DOSE/DURATION           : 70 umol/L
REFERENCE :
   MUREAV Mutation Research.  (Elsevier Science Pub. B.V., POB 211, 1000 AE
   Amsterdam, Netherlands) V.1-    1964-  Volume(issue)/page/year: 89,95,1981
 
TYPE OF TEST            : DNA repair
TEST SYSTEM             : Bacteria - Bacillus subtilis
DOSE/DURATION           : 2 mg/disc
REFERENCE :
   TRENAF Kenkyu Nenpo--Tokyo-toritsu Eisei Kenkyusho.  Annual Report of Tokyo
   Metropolitan Research Laboratory of Public Health.  (Tokyo-toritsu Eisei
   Kenkyusho, 24-1, 3-chome, Hyakunin-cho, Shin-juku-ku, Tokyo 160, Japan) V.1-
      1949/50-  Volume(issue)/page/year: 27,153,1976
 
TYPE OF TEST            : Mutation in microorganisms
TEST SYSTEM             : Microorganism - not otherwise specified
DOSE/DURATION           : 20 ppm
REFERENCE :
   POASAD Proceedings of the Oklahoma Academy of Science.  (Oklahoma Academy of
   Science, c/o Executive Secretary-Treasurer, Southwestern Oklahoma State
   Univ., Weatherford, OK 73096) V.1-    1910/1920-  Volume(issue)/page/year:
   34,114,1953
 
TYPE OF TEST            : Gene conversion and mitotic recombination
TEST SYSTEM             : Yeast - Saccharomyces cerevisiae
DOSE/DURATION           : 10 pph
REFERENCE :
   AGACBH Agents and Actions, A Swiss Journal of Pharmacology.  (Birkhaeuser
   Verlag, POB 133, CH-4010 Basel, Switzerland)  V.1-    1969/70-
   Volume(issue)/page/year: 4,286,1974
 
TYPE OF TEST            : DNA adduct
TEST SYSTEM             : Rodent - mouse Ascites tumor
DOSE/DURATION           : 70 umol/L
REFERENCE :
   MUREAV Mutation Research.  (Elsevier Science Pub. B.V., POB 211, 1000 AE
   Amsterdam, Netherlands) V.1-    1964-  Volume(issue)/page/year: 89,95,1981
 
TYPE OF TEST            : DNA adduct
TEST SYSTEM             : Mammal - species unspecified Lymphocyte
DOSE/DURATION           : 1830 nmol/L
REFERENCE :
   BIPMAA Biopolymers.  (John Wiley & Sons, Inc., 605 Third Ave., New York, NY
   10158)  V.1-    1963-  Volume(issue)/page/year: 12,1099,1973

         *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA ***

NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA :
   NOHS - National Occupational Hazard Survey (1974)
     NOHS Hazard Code - M1469
       No. of Facilities: 4471 (estimated)
       No. of Industries: 35
       No. of Occupations: 34
       No. of Employees: 25916 (estimated)
   
   NOHS - National Occupational Hazard Survey (1974)
     NOHS Hazard Code - T1406
       No. of Facilities: 150 (estimated)
       No. of Industries: 2
       No. of Occupations: 2
       No. of Employees: 664 (estimated)
   
   NOES - National Occupational Exposure Survey (1983)
     NOES Hazard Code - M1469
       No. of Facilities: 5956 (estimated)
       No. of Industries: 29
       No. of Occupations: 51
       No. of Employees: 74208 (estimated)
       No. of Female Employees: 43305 (estimated)

                          *** STATUS IN U.S. ***
 
   EPA GENETOX PROGRAM 1988, Positive/dose response: In vitro SCE-nonhuman
   
   EPA GENETOX PROGRAM 1988, Negative: B subtilis rec assay; S cerevisiae gene
   conversion
   
   EPA GENETOX PROGRAM 1988, Inconclusive: B subtilis rec assay; Histidine
   reversion-Ames test
   
   EPA GENETOX PROGRAM 1988, Inconclusive: D melanogaster Sex-linked lethal
   
   EPA TSCA Section 8(b) CHEMICAL INVENTORY
   
   EPA TSCA Section 8(e) Risk Notification, 8EHQ-0892-8826
   
   EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JUNE 1998

 
                            *** END OF RECORD ***