*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : SS7875000
CHEMICAL NAME : o-Phenylenediamine
CAS REGISTRY NUMBER : 95-54-5
BEILSTEIN REFERENCE NO. : 0606074
REFERENCE : 4-13-00-00038 (Beilstein Handbook Reference)
LAST UPDATED : 199803
DATA ITEMS CITED : 36
MOLECULAR FORMULA : C6-H8-N2
MOLECULAR WEIGHT : 108.16
WISWESSER LINE NOTATION : ZR BZ
COMPOUND DESCRIPTOR : Mutagen
SYNONYMS/TRADE NAMES :
* 2-Aminoaniline
* o-Benzenediamine
* 1,2-Benzenediamine
* C.I. 76010
* C.I. Oxidation Base 16
* o-Diaminobenzene
* EK 1700
* o-Fenylendiamin
* 1,2-Fenylendiamin
* IK 3
* NSC 5354
* Orthamine
* o-Phenylenediamine
* 1,2-Phenylenediamine
* SQ 15500
*** HEALTH HAZARD DATA ***
** ACUTE TOXICITY DATA **
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 510 mg/kg
TOXIC EFFECTS :
Autonomic Nervous System - other (direct) parasympathomimetic
Behavioral - convulsions or effect on seizure threshold
Behavioral - muscle weakness
REFERENCE :
GTPZAB Gigiena Truda i Professional'nye Zabolevaniya. Labor Hygiene and
Occupational Diseases. (V/O Mezhdunarodnaya Kniga, 113095 Moscow, USSR)
V.1-36, 1957-1992. For publisher information, see MTPEEI
Volume(issue)/page/year: 32(9),50,1988
TYPE OF TEST : LC50 - Lethal concentration, 50 percent kill
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 1873 mg/m3
TOXIC EFFECTS :
Peripheral Nerve and Sensation - flaccid paralysis without anesthesia
(usually neuromuscular blockage)
Sense Organs and Special Senses (Olfaction) - effect, not otherwise
specified
Behavioral - convulsions or effect on seizure threshold
REFERENCE :
GISAAA Gigiena i Sanitariya. For English translation, see HYSAAV. (V/O
Mezhdunarodnaya Kniga, 113095 Moscow, USSR) V.1- 1936-
Volume(issue)/page/year: 51(1),73,1986
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Administration onto the skin
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : >5 gm/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
85INA8 "Documentation of the Threshold Limit Values and Biological Exposure
Indices," 5th ed., Cincinnati, OH, American Conference of Governmental
Industrial Hygienists, Inc., 1986 Volume(issue)/page/year: 6,1214,1991
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 516 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
JTEHD6 Journal of Toxicology and Environmental Health. (Hemisphere Pub.,
1025 Vermont Ave., NW, Washington, DC 20005) V.1- 1975/76-
Volume(issue)/page/year: 2,657,1977
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Unreported
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 660 mg/kg
TOXIC EFFECTS :
Peripheral Nerve and Sensation - flaccid paralysis without anesthesia
(usually neuromuscular blockage)
Sense Organs and Special Senses (Olfaction) - effect, not otherwise
specified
Behavioral - convulsions or effect on seizure threshold
REFERENCE :
GISAAA Gigiena i Sanitariya. For English translation, see HYSAAV. (V/O
Mezhdunarodnaya Kniga, 113095 Moscow, USSR) V.1- 1936-
Volume(issue)/page/year: 51(1),73,1986
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 366 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
GISAAA Gigiena i Sanitariya. For English translation, see HYSAAV. (V/O
Mezhdunarodnaya Kniga, 113095 Moscow, USSR) V.1- 1936-
Volume(issue)/page/year: 53(7),62,1988
TYPE OF TEST : LC50 - Lethal concentration, 50 percent kill
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : >91 mg/m3/4H
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
85INA8 "Documentation of the Threshold Limit Values and Biological Exposure
Indices," 5th ed., Cincinnati, OH, American Conference of Governmental
Industrial Hygienists, Inc., 1986 Volume(issue)/page/year: 6,1214,1991
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 245 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
NCISP* National Cancer Institute Screening Program Data Summary,
Developmental Therapeutics Program. (Bethesda, MD 20205)
Volume(issue)/page/year: JAN1986
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 450 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
KJMSAH Kyushu Journal of Medical Science. (Fukuoka, Japan) V.6-15,
1955-64. Discontinued. Volume(issue)/page/year: 13,175,1962
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Unreported
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 336 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
GISAAA Gigiena i Sanitariya. For English translation, see HYSAAV. (V/O
Mezhdunarodnaya Kniga, 113095 Moscow, USSR) V.1- 1936-
Volume(issue)/page/year: 50(6),78,1985
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Mammal - cat
DOSE/DURATION : 250 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
85INA8 "Documentation of the Threshold Limit Values and Biological Exposure
Indices," 5th ed., Cincinnati, OH, American Conference of Governmental
Industrial Hygienists, Inc., 1986 Volume(issue)/page/year: 6,1214,1991
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Administration onto the skin
SPECIES OBSERVED : Rodent - rabbit
DOSE/DURATION : 5 gm/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
JTEHD6 Journal of Toxicology and Environmental Health. (Hemisphere Pub.,
1025 Vermont Ave., NW, Washington, DC 20005) V.1- 1975/76-
Volume(issue)/page/year: 2,657,1977
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - guinea pig
DOSE/DURATION : 360 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
GISAAA Gigiena i Sanitariya. For English translation, see HYSAAV. (V/O
Mezhdunarodnaya Kniga, 113095 Moscow, USSR) V.1- 1936-
Volume(issue)/page/year: 53(7),62,1988
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Unreported
SPECIES OBSERVED : Rodent - guinea pig
DOSE/DURATION : 360 mg/kg
TOXIC EFFECTS :
Peripheral Nerve and Sensation - flaccid paralysis without anesthesia
(usually neuromuscular blockage)
Sense Organs and Special Senses (Olfaction) - effect, not otherwise
specified
Behavioral - convulsions or effect on seizure threshold
REFERENCE :
GISAAA Gigiena i Sanitariya. For English translation, see HYSAAV. (V/O
Mezhdunarodnaya Kniga, 113095 Moscow, USSR) V.1- 1936-
Volume(issue)/page/year: 51(1),73,1986
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Bird - wild bird species
DOSE/DURATION : 133 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
AECTCV Archives of Environmental Contamination and Toxicology.
(Springer-Verlag New York, Inc., Service Center, 44 Hartz Way, Secaucus, NJ
070944) V.1- 1973- Volume(issue)/page/year: 12,355,1983
** MUTATION DATA **
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 1 gm/L
REFERENCE :
JIMMBG Journal of Immunological Methods. (Elsevier Science Pub. Co., Inc.,
52 Vanderbilt Ave., New York, NY 10017) V.1- 1971-
Volume(issue)/page/year: 36,55,1980
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 1 umol/plate
REFERENCE :
JTSCDR Journal of Toxicological Sciences. (Japanese Soc. of Toxicological
Sciences, 4th Floor, Gakkai Center Bldg., 4-16, Yayoi 2-chome, Bunkyo-ku,
Tokyo 113, Japan) V.1- 1976- Volume(issue)/page/year: 7,61,1982
TYPE OF TEST : Mutation test systems - not otherwise specified
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 140 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 192,239,1987
TYPE OF TEST : Phage inhibition capacity
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 125 ug/well
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 260,349,1991
TYPE OF TEST : DNA inhibition
TEST SYSTEM : Human HeLa cell
DOSE/DURATION : 7 mmol/L
REFERENCE :
CRNGDP Carcinogenesis (London). (Oxford Univ. Press, Pinkhill House,
Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1980-
Volume(issue)/page/year: 13,2389,1992
TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Rodent - rat Liver
DOSE/DURATION : 100 umol/L
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 5,803,1983
TYPE OF TEST : Micronucleus test
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 216 mg/kg/24H
REFERENCE :
ARTODN Archives of Toxicology. (Springer-Verlag, Heidelberger Pl. 3, D-1000
Berlin 33, Fed. Rep. Ger.) V.32- 1974- Volume(issue)/page/year:
43,249,1980
TYPE OF TEST : Micronucleus test
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 216 mg/kg/24H
REFERENCE :
ARTODN Archives of Toxicology. (Springer-Verlag, Heidelberger Pl. 3, D-1000
Berlin 33, Fed. Rep. Ger.) V.32- 1974- Volume(issue)/page/year:
43,249,1980
TYPE OF TEST : DNA inhibition
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 200 mg/kg
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 46,305,1977
TYPE OF TEST : Micronucleus test
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - hamster
DOSE/DURATION : 432 mg/kg/24H
REFERENCE :
ARTODN Archives of Toxicology. (Springer-Verlag, Heidelberger Pl. 3, D-1000
Berlin 33, Fed. Rep. Ger.) V.32- 1974- Volume(issue)/page/year:
43,249,1980
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Ovary
DOSE/DURATION : 187 mg/L
REFERENCE :
EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E.
11th St., New York, NY 10003) V.10- 1987- Volume(issue)/page/year:
27,67,1996
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Lung
DOSE/DURATION : 1 mg/L
REFERENCE :
ATSUDG Archives of Toxicology, Supplement. (Springer-Verlag New York, Inc.,
Service Center, 44 Hartz Way, Secaucus, NJ 07094) No.1- 1978-
Volume(issue)/page/year: 4,41,1980
TYPE OF TEST : Micronucleus test
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - guinea pig
DOSE/DURATION : 216 mg/kg/24H
REFERENCE :
ARTODN Archives of Toxicology. (Springer-Verlag, Heidelberger Pl. 3, D-1000
Berlin 33, Fed. Rep. Ger.) V.32- 1974- Volume(issue)/page/year:
43,249,1980
*** REVIEWS ***
ACGIH TLV-Animal carcinogen
DTLVS* The Threshold Limit Values (TLVs) and Biological Exposure Indices
(BEIs) booklet issues by American Conference of Governmental Industrial
Hygienists (ACGIH), Cincinnati, OH, 1996 Volume(issue)/page/year:
TLV/BEI,1997
ACGIH TLV-TWA 0.1 mg/m3
DTLVS* The Threshold Limit Values (TLVs) and Biological Exposure Indices
(BEIs) booklet issues by American Conference of Governmental Industrial
Hygienists (ACGIH), Cincinnati, OH, 1996 Volume(issue)/page/year:
TLV/BEI,1997
*** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA ***
NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA :
NOES - National Occupational Exposure Survey (1983)
NOES Hazard Code - X4334
No. of Facilities: 342 (estimated)
No. of Industries: 2
No. of Occupations: 4
No. of Employees: 3076 (estimated)
No. of Female Employees: 1372 (estimated)
*** STATUS IN U.S. ***
EPA GENETOX PROGRAM 1988, Negative: Rodent dominant lethal; N
crassa-aneuploidy
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA 8(a) PRELIMINARY ASSESSMENT INFORMATION, FINAL RULE
FEREAC Federal Register. (U.S. Government Printing Office, Supt. of
Documents, Washington, DC 20402) V.1- 1936- Volume(issue)/page/year:
47,26992,82
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JUNE 1998
*** END OF RECORD ***