*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : ST2975000
CHEMICAL NAME : o-Phenylenediamine, 4-nitro-
CAS REGISTRY NUMBER : 99-56-9
BEILSTEIN REFERENCE NO. : 0608106
REFERENCE : 4-13-00-00075 (Beilstein Handbook Reference)
LAST UPDATED : 199710
DATA ITEMS CITED : 55
MOLECULAR FORMULA : C6-H7-N3-O2
MOLECULAR WEIGHT : 153.16
WISWESSER LINE NOTATION : ZR BZ DNW
COMPOUND DESCRIPTOR : Tumorigen
Mutagen
Reproductive Effector
SYNONYMS/TRADE NAMES :
* 2-Amino-4-nitroaniline
* C.I. 76020
* 1,2-Diamino-4-nitrobenzene
* 3,4-Diaminonitrobenzene
* NCI-C03941
* 4NDB
* 4-Nitro-1,2-benzenediamine
* 4-Nitro-1,2-diaminobenzene
* 4-Nitro-1,2-fenylendiamin
* p-Nitro-o-phenylenediamine
* 4-Nitro-o-phenylene-diamine
* 4-Nitro-1,2-phenylenediamine
* 4-NO
* 4-Nop
* 4-Nopd
* 4-N-o-Pda
*** HEALTH HAZARD DATA ***
** ACUTE TOXICITY DATA **
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 681 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
NCILB* Progress Report for Contract No. NIH-NCI-E-C-72-3252, Submitted to
the National Cancer Institute by Litton Bionetics, Inc. (Bethesda, MD)
Volume(issue)/page/year: NCI-E-C-72-3252,1973
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : >1600 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
JTEHD6 Journal of Toxicology and Environmental Health. (Hemisphere Pub.,
1025 Vermont Ave., NW, Washington, DC 20005) V.1- 1975/76-
Volume(issue)/page/year: 2,657,1977
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 681 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
NCILB* Progress Report for Contract No. NIH-NCI-E-C-72-3252, Submitted to
the National Cancer Institute by Litton Bionetics, Inc. (Bethesda, MD)
Volume(issue)/page/year: NCI-E-C-72-3252,1973
** REPRODUCTIVE DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE : 3 gm/kg
SEX/DURATION : male 30 day(s) pre-mating
TOXIC EFFECTS :
Reproductive - Effects on Embryo or Fetus - fetal death
REFERENCE :
SheCW# Personal Communication from C.W. Sheu, Genetic Toxicology Branch,
FDA, Mar. 25, 1977 Volume(issue)/page/year: 25MAR1977
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - mouse
DOSE : 2560 mg/kg
SEX/DURATION : female 6-15 day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death,
e.g., stunted fetus)
Reproductive - Specific Developmental Abnormalities - craniofacial
(including nose and tongue)
Reproductive - Specific Developmental Abnormalities - cardiovascular
(circulatory) system
REFERENCE :
TJADAB Teratology, The International Journal of Abnormal Development. (Alan
R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.1- 1968-
Volume(issue)/page/year: 19,37A,1979
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - mouse
DOSE : 7680 mg/kg
SEX/DURATION : female 6-15 day(s) after conception
TOXIC EFFECTS :
Reproductive - Fertility - litter size (e.g. # fetuses per litter; measured
before birth)
REFERENCE :
TJADAB Teratology, The International Journal of Abnormal Development. (Alan
R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.1- 1968-
Volume(issue)/page/year: 24,253,1981
** MUTATION DATA **
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 1 ug/plate
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 8(Suppl
7),1,1986
TYPE OF TEST : Mutation test systems - not otherwise specified
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 5 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 192,239,1987
TYPE OF TEST : DNA repair
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 10 mg/L
REFERENCE :
CRNGDP Carcinogenesis (London). (Oxford Univ. Press, Pinkhill House,
Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1980-
Volume(issue)/page/year: 2,189,1981
TYPE OF TEST : Phage inhibition capacity
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 400 ug/plate
REFERENCE :
MGBUA3 Microbial Genetics Bulletin. (Ohio State Univ., College of
Biological Science, Dept. of Microbiology, 484 W. 12th Ave., Columbus, OH
43210) No.1- 1950- Volume(issue)/page/year: 41,3,1976
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Bacillus subtilis
DOSE/DURATION : 50 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 95,191,1982
TYPE OF TEST : Specific locus test
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Insect - Drosophila melanogaster
DOSE/DURATION : 2 mmol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 341,161,1995
TYPE OF TEST : Sex chromosome loss and nondisjunction
ROUTE OF EXPOSURE : Parenteral
TEST SYSTEM : Insect - Drosophila melanogaster
DOSE/DURATION : 5 mmol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 56,31,1977
TYPE OF TEST : Sex chromosome loss and nondisjunction
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Insect - Drosophila melanogaster
DOSE/DURATION : 1200 umol/L/3D
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 48,181,1977
TYPE OF TEST : Gene conversion and mitotic recombination
TEST SYSTEM : Yeast - Saccharomyces cerevisiae
DOSE/DURATION : 500 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 78,243,1980
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Mold - Aspergillus nidulans
DOSE/DURATION : 200 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 97,293,1982
TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Human HeLa cell
DOSE/DURATION : 1 mmol/L
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
38,2621,1978
TYPE OF TEST : Morphological transformation
TEST SYSTEM : Rodent - rat Embryo
DOSE/DURATION : 80 ug/plate
REFERENCE :
JJATDK JAT, Journal of Applied Toxicology. (John Wiley & Sons Ltd., Baffins
Lane, Chichester, W. Sussex PO19 1UD, UK) V.1- 1981-
Volume(issue)/page/year: 1,190,1981
TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Rodent - rat Liver
DOSE/DURATION : 500 ug/L
REFERENCE :
EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E.
11th St., New York, NY 10003) V.10- 1987- Volume(issue)/page/year:
26,147,1995
TYPE OF TEST : Micronucleus test
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 1 gm/kg
REFERENCE :
EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E.
11th St., New York, NY 10003) V.10- 1987- Volume(issue)/page/year:
14,207,1989
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Rodent - mouse Lymphocyte
DOSE/DURATION : 13100 ug/L
REFERENCE :
EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E.
11th St., New York, NY 10003) V.10- 1987- Volume(issue)/page/year:
12(Suppl 13),37,1988
TYPE OF TEST : Morphological transformation
TEST SYSTEM : Rodent - mouse Embryo
DOSE/DURATION : 10 umol/L/24H
REFERENCE :
NATUAS Nature. (Nature Subscription Dept., POB 1018, Manasguan, NJ 08736)
V.1- 1869- Volume(issue)/page/year: 260,368,1976
TYPE OF TEST : Morphological transformation
TEST SYSTEM : Rodent - mouse Cells - not otherwise specified
DOSE/DURATION : 10 umol/L
REFERENCE :
NATUAS Nature. (Nature Subscription Dept., POB 1018, Manasguan, NJ 08736)
V.1- 1869- Volume(issue)/page/year: 260,368,1976
TYPE OF TEST : DNA damage
TEST SYSTEM : Rodent - mouse Lymphocyte
DOSE/DURATION : 104 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 203,155,1988
TYPE OF TEST : Mutation test systems - not otherwise specified
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 250 mg/kg
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 343,157,1995
TYPE OF TEST : Dominant lethal test
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 3000 mg/kg/10W (Intermittent)
REFERENCE :
SheCW# Personal Communication from C.W. Sheu, Genetic Toxicology Branch,
FDA, Mar. 25, 1977 Volume(issue)/page/year: 25MAR1977
TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Rodent - mouse Lymphocyte
DOSE/DURATION : 50 mg/L
REFERENCE :
JEPTDQ Journal of Environmental Pathology and Toxicology. (Park Forest
South, IL) V.1-5(3), 1977-81(?). For publisher information, see JEPOEC.
Volume(issue)/page/year: 1(1),87,1977
TYPE OF TEST : Morphological transformation
TEST SYSTEM : Rodent - hamster Embryo
DOSE/DURATION : 500 ug/L
REFERENCE :
NCIMAV National Cancer Institute, Monograph. (U.S. Government Printing
Office, Supt. of Documents, Washington, DC 20402) No.1- 1959-
Volume(issue)/page/year: 58,243,1981
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Ovary
DOSE/DURATION : 138 mg/L
REFERENCE :
EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E.
11th St., New York, NY 10003) V.10- 1987- Volume(issue)/page/year:
27,67,1996
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Fibroblast
DOSE/DURATION : 25 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 40,47,1976
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Cells - not otherwise specified
DOSE/DURATION : 10 umol/L/24H
REFERENCE :
NATUAS Nature. (Nature Subscription Dept., POB 1018, Manasguan, NJ 08736)
V.1- 1869- Volume(issue)/page/year: 260,368,1976
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Cells - not otherwise specified
DOSE/DURATION : 10 umol/L
REFERENCE :
NATUAS Nature. (Nature Subscription Dept., POB 1018, Manasguan, NJ 08736)
V.1- 1869- Volume(issue)/page/year: 260,368,1976
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Lung
DOSE/DURATION : 40 mg/L
REFERENCE :
GMCRDC Gann Monograph on Cancer Research. (Plenum Pub. Corp., 233 Spring
St., New York, NY 10013) No. 11- 1971- Volume(issue)/page/year:
27,95,1981
TYPE OF TEST : Sister chromatid exchange
TEST SYSTEM : Rodent - hamster Ovary
DOSE/DURATION : 25 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 56,207,1977
*** REVIEWS ***
IARC Cancer Review:Animal Inadequate Evidence
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 16,63,1978
IARC Cancer Review:Human No Adequate Data
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 16,63,1978
IARC Cancer Review:Group 3
IMSUDL IARC Monographs, Supplement. (WHO Publications Centre USA, 49
Sheridan Ave., Albany, NY 12210) No.1- 1979- Volume(issue)/page/year:
7,56,1987
TOXICOLOGY REVIEW
JACTDZ Journal of the American College of Toxicology. (Mary Ann Liebert,
Inc., 1651 Third Ave., New York, NY 10128) V.1-12, 1982-1993. Discontinued.
Volume(issue)/page/year: 4(3),161,1985
*** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA ***
NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA :
NOES - National Occupational Exposure Survey (1983)
NOES Hazard Code - A1601
No. of Facilities: 2065 (estimated)
No. of Industries: 1
No. of Occupations: 1
No. of Employees: 17652 (estimated)
No. of Female Employees: 11624 (estimated)
*** STATUS IN U.S. ***
EPA GENETOX PROGRAM 1988, Positive: Body fluid assay; SHE-clonal assay
EPA GENETOX PROGRAM 1988, Positive: Cell transform.-C3H/10T1/2
EPA GENETOX PROGRAM 1988, Positive: Cell transform.-RLV F344 rat embryo
EPA GENETOX PROGRAM 1988, Positive: Histidine reversion-Ames test
EPA GENETOX PROGRAM 1988, Positive: D melanogaster Sex-linked lethal
EPA GENETOX PROGRAM 1988, Positive/dose response: In vitro
cytogenetics-nonhuman
EPA GENETOX PROGRAM 1988, Positive/dose response: In vitro SCE-nonhuman
EPA GENETOX PROGRAM 1988, Negative: Carcinogenicity-mouse/rat; N
crassa-aneuploidy
EPA GENETOX PROGRAM 1988, Negative: Sperm morphology-mouse; S
cerevisiae-reversion
EPA GENETOX PROGRAM 1988, Inconclusive: Rodent dominant lethal; Mammalian
micronucleus
EPA GENETOX PROGRAM 1988, Inconclusive: In vitro UDS-human fibroblast
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA 8(a) PRELIMINARY ASSESSMENT INFORMATION, FINAL RULE
FEREAC Federal Register. (U.S. Government Printing Office, Supt. of
Documents, Washington, DC 20402) V.1- 1936- Volume(issue)/page/year:
47,26992,82
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JUNE 1998
NCI Carcinogenesis Bioassay (feed);no evidence:mouse,rat
NCITR* National Cancer Institute Carcinogenesis Technical Report Series.
(Bethesda, MD) No.0-205. For publisher information, see NTPTR*.
Volume(issue)/page/year: NCI-TR-180,79
*** END OF RECORD ***