*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : TB4760000
CHEMICAL NAME : Phosphoramidothioic acid, N-acetyl-, O,S-dimethyl
ester
CAS REGISTRY NUMBER : 30560-19-1
BEILSTEIN REFERENCE NO. : 1936365
LAST UPDATED : 199710
DATA ITEMS CITED : 40
MOLECULAR FORMULA : C4-H10-N-O3-P-S
MOLECULAR WEIGHT : 183.18
WISWESSER LINE NOTATION : 1VMPO&S1&O1
COMPOUND DESCRIPTOR : Agricultural Chemical
Mutagen
Reproductive Effector
SYNONYMS/TRADE NAMES :
* Acephat
* Acephate
* Acetamidophos
* Acetylphosphoramidothioic acid O,S-dimethyl ester
* Chevron RE 12,420
* O,S-Dimethylacetylphosphoroamidothioate
* ENT 27822
* N-(Methoxy(methylthio)phosphinoyl)acetamide
* Orthene
* Orthene-755
* Ortho 12420
* Ortho 124120
* Ortran
* Ortril
* RE 12420
* 75 SP
*** HEALTH HAZARD DATA ***
** ACUTE TOXICITY DATA **
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 700 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
USXXAM United States Patent Document. (U.S. Patent Office, Box 9,
Washington, DC 20231) Volume(issue)/page/year: #3716600
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Administration onto the skin
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : >2500 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
FAATDF Fundamental and Applied Toxicology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1981- Volume(issue)/page/year:
7,299,1986
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 233 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
CHYCDW Zhonghua Yufangyixue Zazhi. Chinese Journal of Preventive Medicine.
(China International Book Trading Corp., POB 2820, Beijing, Peop. Rep.
China) Beginning history not known. Volume(issue)/page/year: 14,226,1980
TYPE OF TEST : LCLo - Lowest published lethal concentration
ROUTE OF EXPOSURE : Inhalation
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 2200 mg/m3/5H
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
45,232,1978
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Mammal - dog
DOSE/DURATION : 681 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
GUCHAZ Guide to the Chemicals Used in Crop Protection. (Information Canada,
171 Slater St., Ottawa, Ont., Canada) Volume(issue)/page/year: 6,1,1973
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Administration onto the skin
SPECIES OBSERVED : Rodent - rabbit
DOSE/DURATION : 2 gm/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
85DPAN "Wirksubstanzen der Pflanzenschutz und Schadlingsbekampfungsmittel,"
Perkow, W., Berlin, Verlag Paul Parey, 1971-1976 Volume(issue)/page/year:
-,-,1971/1976
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Bird - chicken
DOSE/DURATION : 800 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
NRTXDN Neurotoxicology. (Intox Press, Inc., POB 34075, Little Rock, AR
72203) V.1- 1979- Volume(issue)/page/year: 11,483,1990
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Unreported
SPECIES OBSERVED : Bird - chicken
DOSE/DURATION : 568 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
DEVEAA Defense des Vegetaux. (Federation Nationale des Groupements de
Protection des Cultures, 149, rue de Bercy, 75595 Paris Cedex, 12, France)
V.1- 1947- Volume(issue)/page/year: 28,231,1974
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Bird - duck
DOSE/DURATION : 350 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
28ZEAL "Pesticide Index," Frear, E.H., ed., State College, PA, College
Science Pub., 1969 Volume(issue)/page/year: 5,4,1976
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Mammal - species unspecified
DOSE/DURATION : 321 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
AECTCV Archives of Environmental Contamination and Toxicology.
(Springer-Verlag New York, Inc., Service Center, 44 Hartz Way, Secaucus, NJ
070944) V.1- 1973- Volume(issue)/page/year: 13,483,1984
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Bird - domestic
DOSE/DURATION : 106 mg/kg
TOXIC EFFECTS :
Behavioral - convulsions or effect on seizure threshold
Behavioral - ataxia
Lungs, Thorax, or Respiration - dyspnea
REFERENCE :
AECTCV Archives of Environmental Contamination and Toxicology.
(Springer-Verlag New York, Inc., Service Center, 44 Hartz Way, Secaucus, NJ
070944) V.1- 1973- Volume(issue)/page/year: 10,185,1981
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Bird - wild bird species
DOSE/DURATION : 140 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
PEMNDP Pesticide Manual. (The British Crop Protection Council, 20 Bridport
Rd., Thornton Heath CR4 7QG, UK) V.1- 1968- Volume(issue)/page/year:
9,4,1991
** OTHER MULTIPLE DOSE TOXICITY DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 14580 mg/kg/90D-C
TOXIC EFFECTS :
Nutritional and Gross Metabolic - weight loss or decreased weight gain
Biochemical - Enzyme inhibition, induction, or change in blood or tissue
levels - true cholinesterase
REFERENCE :
CHYCDW Zhonghua Yufangyixue Zazhi. Chinese Journal of Preventive Medicine.
(China International Book Trading Corp., POB 2820, Beijing, Peop. Rep.
China) Beginning history not known. Volume(issue)/page/year: 14,226,1980
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 1050 mg/kg/15W-I
TOXIC EFFECTS :
Brain and Coverings - other degenerative changes
Autonomic Nervous System - sympathomimetic
Biochemical - Metabolism (Intermediary) - amino acids (including renal
excretion)
REFERENCE :
ENVRAL Environmental Research. (Academic Press, Inc., 1 E. First St.,
Duluth, MN 55802) V.1- 1967- Volume(issue)/page/year: 43,342,1987
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 33600 mg/kg/8W-I
TOXIC EFFECTS :
Liver - other changes
Biochemical - Metabolism (Intermediary) - other proteins
REFERENCE :
IJEBA6 Indian Journal of Experimental Biology. (Publications & Information
Directorate, CSIR, Hillside Rd., New Delhi 110 012, India) V.1- 1963-
Volume(issue)/page/year: 21,684,1983
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 15 mg/kg/5D-C
TOXIC EFFECTS :
Brain and Coverings - other degenerative changes
Biochemical - Enzyme inhibition, induction, or change in blood or tissue
levels - true cholinesterase
REFERENCE :
TOLED5 Toxicology Letters. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1977- Volume(issue)/page/year: 24,65,1985
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 13500 mg/kg/90D-I
TOXIC EFFECTS :
Liver - other changes
REFERENCE :
HOEKAN Hokkaidoritsu Eisei Kenkyushoho. Report of the Hokkaido Institute of
Public Health. (Nishi- 12-chome, Kita-19-jo, Kita-ku, Sapporo 060, Japan)
No.1- 1951- Volume(issue)/page/year: 22,48,1922
** REPRODUCTIVE DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE : 150 mg/kg
SEX/DURATION : male 5 day(s) pre-mating
TOXIC EFFECTS :
Reproductive - Paternal Effects - spermatogenesis (incl. genetic material,
sperm morphology, motility, and count)
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 223,287,1989
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE : 200 mg/kg
SEX/DURATION : male 5 day(s) pre-mating
TOXIC EFFECTS :
Reproductive - Fertility - post-implantation mortality (e.g. dead and/or
resorbed implants per total number of implants)
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 223,287,1989
** MUTATION DATA **
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 3 mg/plate
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: PB80-133226
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 50 mg/plate
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 116,185,1983
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 5 mg/plate
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 28,405,1975
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Yeast - Saccharomyces cerevisiae
DOSE/DURATION : 3 pph
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: PB84-138973
TYPE OF TEST : Gene conversion and mitotic recombination
TEST SYSTEM : Yeast - Saccharomyces cerevisiae
DOSE/DURATION : 50000 ppm
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: PB80-133226
TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Human Fibroblast
DOSE/DURATION : 1 gm/L
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: PB80-133226
TYPE OF TEST : Micronucleus test
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 500 mg/kg/24H
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 223,287,1989
TYPE OF TEST : Cytogenetic analysis
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 150 mg/kg
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 223,287,1989
TYPE OF TEST : Dominant lethal test
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 250 mg/kg/5D (Continuous)
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 223,287,1989
TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Rodent - mouse Lymphocyte
DOSE/DURATION : 1 gm/L
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: PB84-138973
TYPE OF TEST : Sperm Morphology
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 150 mg/kg/5D (Continuous)
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 223,287,1989
TYPE OF TEST : Sister chromatid exchange
TEST SYSTEM : Rodent - hamster Ovary
DOSE/DURATION : 2 gm/L
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: PB84-138973
*** U.S. STANDARDS AND REGULATIONS ***
EPA FIFRA 1988 PESTICIDE SUBJECT TO REGISTRATION OR RE-REGISTRATION
FEREAC Federal Register. (U.S. Government Printing Office, Supt. of
Documents, Washington, DC 20402) V.1- 1936- Volume(issue)/page/year:
54,7740,1989
*** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA ***
NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA :
NOES - National Occupational Exposure Survey (1983)
NOES Hazard Code - T0473
No. of Facilities: 196 (estimated)
No. of Industries: 2
No. of Occupations: 1
No. of Employees: 770 (estimated)
No. of Female Employees: 378 (estimated)
*** STATUS IN U.S. ***
EPA GENETOX PROGRAM 1988, Positive: S cerevisiae-homozygosis
EPA GENETOX PROGRAM 1988, Negative: N crassa-aneuploidy; Histidine
reversion-Ames test
EPA GENETOX PROGRAM 1988, Negative: D melanogaster Sex-linked lethal
EPA GENETOX PROGRAM 1988, Negative: In vitro UDS-human fibroblast; TRP
reversion
EPA GENETOX PROGRAM 1988, Inconclusive: B subtilis rec assay; E coli polA
without S9
On EPA IRIS database
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JUNE 1998
*** END OF RECORD ***