*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : TI2500000
CHEMICAL NAME : Phthalic acid, mono-(2-ethylhexyl) ester
CAS REGISTRY NUMBER : 4376-20-9
LAST UPDATED : 199712
DATA ITEMS CITED : 40
MOLECULAR FORMULA : C16-H22-O4
MOLECULAR WEIGHT : 278.38
WISWESSER LINE NOTATION : QVR BVO1Y4&2
COMPOUND DESCRIPTOR : Mutagen
Reproductive Effector
Primary Irritant
SYNONYMS/TRADE NAMES :
* BAR 1
* 1,2-Benzenedicarboxylic acid, mono(2-ethylhexyl) ester
* 2-Ethylhexyl hydrogen phthalate
* Monoethylhexyl phthalate
* Mono(2-ethylhexyl)phthalate
* Phthalic acid, 2-ethylhexyl ester
*** HEALTH HAZARD DATA ***
** SKIN/EYE IRRITATION DATA **
TYPE OF TEST : Standard Draize test
ROUTE OF EXPOSURE : Administration onto the skin
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 100 mg
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: PB250-102
** ACUTE TOXICITY DATA **
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 1340 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
45,250,1978
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 415 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: PB250-102
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 150 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: PB250-102
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 240 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: PB250-102
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 208 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: PB250-102
** OTHER MULTIPLE DOSE TOXICITY DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 7 gm/kg/14D-I
TOXIC EFFECTS :
Liver - changes in liver weight
Biochemical - Enzyme inhibition, induction, or change in blood or tissue
levels - dehydrogenases
Biochemical - Enzyme inhibition, induction, or change in blood or tissue
levels - other transferases
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
72,46,1984
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 8400 mg/kg/1W-C
TOXIC EFFECTS :
Liver - changes in liver weight
Blood - changes in serum composition (e.g. TP, bilirubin, cholesterol)
Biochemical - Metabolism (Intermediary) - lipids including transport
REFERENCE :
TOLED5 Toxicology Letters. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1977- Volume(issue)/page/year: 14,79,1982
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 168 mg/kg/28D-C
TOXIC EFFECTS :
Blood - pigmented or nucleated red blood cells
Blood - changes in leukocyte (WBC) count
REFERENCE :
AECTCV Archives of Environmental Contamination and Toxicology.
(Springer-Verlag New York, Inc., Service Center, 44 Hartz Way, Secaucus, NJ
070944) V.1- 1973- Volume(issue)/page/year: 10,271,1981
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 675 mg/kg/90D-C
TOXIC EFFECTS :
Biochemical - Enzyme inhibition, induction, or change in blood or tissue
levels - dehydrogenases
REFERENCE :
AECTCV Archives of Environmental Contamination and Toxicology.
(Springer-Verlag New York, Inc., Service Center, 44 Hartz Way, Secaucus, NJ
070944) V.1- 1973- Volume(issue)/page/year: 10,271,1981
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 6825 mg/kg/26W-C
TOXIC EFFECTS :
Liver - changes in liver weight
REFERENCE :
AECTCV Archives of Environmental Contamination and Toxicology.
(Springer-Verlag New York, Inc., Service Center, 44 Hartz Way, Secaucus, NJ
070944) V.1- 1973- Volume(issue)/page/year: 10,271,1981
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - hamster
DOSE/DURATION : 7 gm/kg/14D-I
TOXIC EFFECTS :
Liver - changes in liver weight
Biochemical - Enzyme inhibition, induction, or change in blood or tissue
levels - hepatic microsomal mixed oxidase (dealkylation, hydroxylation,
etc.)
Biochemical - Enzyme inhibition, induction, or change in blood or tissue
levels - other transferases
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
72,46,1984
** REPRODUCTIVE DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE : 4500 mg/kg
SEX/DURATION : female 6-15 day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Newborn - other neonatal measures or effects
REFERENCE :
BECTA6 Bulletin of Environmental Contamination and Toxicology.
(Springer-Verlag New York, Inc., Service Center, 44 Hartz Way, Secaucus, NJ
07094) V.1- 1966- Volume(issue)/page/year: 27,181,1981
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE : 6320 ug/kg
SEX/DURATION : male 7 day(s) pre-mating
TOXIC EFFECTS :
Reproductive - Paternal Effects - testes, epididymis, sperm duct
REFERENCE :
TXCYAC Toxicology. (Elsevier Scientific Pub. Ireland, Ltd., POB 85,
Limerick, Ireland) V.1- 1973- Volume(issue)/page/year: 15,197,1980
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE : 3750 mg/kg
SEX/DURATION : male 5 day(s) pre-mating
TOXIC EFFECTS :
Reproductive - Paternal Effects - spermatogenesis (incl. genetic material,
sperm morphology, motility, and count)
REFERENCE :
APTOA6 Acta Pharmacologica et Toxicologica. (Copenhagen, Denmark) V.1-59,
1945-86. For publisher information, see PHTOEH Volume(issue)/page/year:
58,225,1986
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE : 1 gm/kg
SEX/DURATION : male 20 day(s) pre-mating
TOXIC EFFECTS :
Reproductive - Paternal Effects - prostate, seminal vesicle, Cowper's gland,
accessory glands
REFERENCE :
EVHPAZ EHP, Environmental Health Perspectives. (U.S. Government Printing
Office, Supt of Documents, Washington, DC 20402) No.1- 1972-
Volume(issue)/page/year: 45,85,1982
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE : 500 mg/kg
SEX/DURATION : male 10 day(s) pre-mating
TOXIC EFFECTS :
Reproductive - Paternal Effects - other effects on male
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
62,121,1982
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE : 4360 mg/kg
SEX/DURATION : female 6-13 day(s) after conception
TOXIC EFFECTS :
Reproductive - Fertility - litter size (e.g. # fetuses per litter; measured
before birth)
REFERENCE :
TCMUD8 Teratogenesis, Carcinogenesis, and Mutagenesis. (Alan R. Liss, Inc.,
41 E. 11th St., New York, NY 10003) V.1- 1980- Volume(issue)/page/year:
7,29,1987
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE : 100 mg/kg
SEX/DURATION : female 7 day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death,
e.g., stunted fetus)
REFERENCE :
JEPTDQ Journal of Environmental Pathology and Toxicology. (Park Forest
South, IL) V.1-5(3), 1977-81(?). For publisher information, see JEPOEC.
Volume(issue)/page/year: 4(2-3),533,1980
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE : 350 mg/kg
SEX/DURATION : male 1 day(s) pre-mating
TOXIC EFFECTS :
Reproductive - Fertility - pre-implantation mortality (e.g. reduction in
number of implants per female; total number of implants per corpora lutea)
REFERENCE :
OEKSDJ Osaka-furitsu Koshu Eisei Kenkyusho Kenkyu Hokoku, Shokuhin Eisei
Hen. Research Reports of the Osaka Prefectural Institute of Public Health,
Food Sanitation Section. (Osaka-furitsu Koshu Eisei Kenkyusho, 1-3-69
Nakamichi, Higashinari-ku, Osaka 537, Japan) No.1- 1970-
Volume(issue)/page/year: 10,1,1979
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE : 100 mg/kg
SEX/DURATION : female 8 day(s) after conception
TOXIC EFFECTS :
Reproductive - Specific Developmental Abnormalities - musculoskeletal system
REFERENCE :
JEPTDQ Journal of Environmental Pathology and Toxicology. (Park Forest
South, IL) V.1-5(3), 1977-81(?). For publisher information, see JEPOEC.
Volume(issue)/page/year: 4(2-3),533,1980
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE : 1241 mg/kg
SEX/DURATION : female 1-17 day(s) after conception
TOXIC EFFECTS :
Reproductive - Fertility - post-implantation mortality (e.g. dead and/or
resorbed implants per total number of implants)
Reproductive - Effects on Embryo or Fetus - other effects to embryo
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: PB91-185926
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - hamster
DOSE : 9 gm/kg
SEX/DURATION : male 9 day(s) pre-mating
TOXIC EFFECTS :
Reproductive - Paternal Effects - testes, epididymis, sperm duct
REFERENCE :
TOLED5 Toxicology Letters. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1977- Volume(issue)/page/year: 11,141,1982
** MUTATION DATA **
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 1250 ug/plate
REFERENCE :
EVHPAZ EHP, Environmental Health Perspectives. (U.S. Government Printing
Office, Supt of Documents, Washington, DC 20402) No.1- 1972-
Volume(issue)/page/year: 45,119,1982
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 700 mg/L
REFERENCE :
EVHPAZ EHP, Environmental Health Perspectives. (U.S. Government Printing
Office, Supt of Documents, Washington, DC 20402) No.1- 1972-
Volume(issue)/page/year: 45,119,1982
TYPE OF TEST : DNA repair
TEST SYSTEM : Bacteria - Bacillus subtilis
DOSE/DURATION : 400 ug/disc
REFERENCE :
EVHPAZ EHP, Environmental Health Perspectives. (U.S. Government Printing
Office, Supt of Documents, Washington, DC 20402) No.1- 1972-
Volume(issue)/page/year: 45,119,1982
TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Rodent - rat Liver
DOSE/DURATION : 200 umol/L/24H
REFERENCE :
PAACA3 Proceedings of the American Association for Cancer Research. (Waverly
Press, 428 E. Preston St., Baltimore, MD 21202) V.1- 1954-
Volume(issue)/page/year: 27,148,1986
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - rat Liver
DOSE/DURATION : 2 mmol/L
REFERENCE :
EVHPAZ EHP, Environmental Health Perspectives. (U.S. Government Printing
Office, Supt of Documents, Washington, DC 20402) No.1- 1972-
Volume(issue)/page/year: 65,263,1986
TYPE OF TEST : Specific locus test
ROUTE OF EXPOSURE : Parenteral
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 25 mg/kg
REFERENCE :
EVHPAZ EHP, Environmental Health Perspectives. (U.S. Government Printing
Office, Supt of Documents, Washington, DC 20402) No.1- 1972-
Volume(issue)/page/year: 65,249,1986
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Rodent - hamster Lung
DOSE/DURATION : 750 mg/L
REFERENCE :
ESKGA2 Eisei Kagaku. Hygienic Chemistry. (Nippon Yakugakkai, 2-12-15
Shibuya, Shibuya-ku, Tokyo 150, Japan) V.1- 1953-
Volume(issue)/page/year: 28,P36,1982
TYPE OF TEST : Morphological transformation
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Rodent - hamster
DOSE/DURATION : 375 mg/kg
REFERENCE :
EVHPAZ EHP, Environmental Health Perspectives. (U.S. Government Printing
Office, Supt of Documents, Washington, DC 20402) No.1- 1972-
Volume(issue)/page/year: 45,119,1982
TYPE OF TEST : Cytogenetic analysis
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Rodent - hamster
DOSE/DURATION : 375 mg/kg
REFERENCE :
EVHPAZ EHP, Environmental Health Perspectives. (U.S. Government Printing
Office, Supt of Documents, Washington, DC 20402) No.1- 1972-
Volume(issue)/page/year: 45,119,1982
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Ovary
DOSE/DURATION : 1 mmol/L
REFERENCE :
EVHPAZ EHP, Environmental Health Perspectives. (U.S. Government Printing
Office, Supt of Documents, Washington, DC 20402) No.1- 1972-
Volume(issue)/page/year: 65,263,1986
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Lung
DOSE/DURATION : 1 gm/L
REFERENCE :
ESKGA2 Eisei Kagaku. Hygienic Chemistry. (Nippon Yakugakkai, 2-12-15
Shibuya, Shibuya-ku, Tokyo 150, Japan) V.1- 1953-
Volume(issue)/page/year: 28,P36,1982
TYPE OF TEST : Sister chromatid exchange
TEST SYSTEM : Rodent - hamster Lung
DOSE/DURATION : 25 mg/L
REFERENCE :
EVHPAZ EHP, Environmental Health Perspectives. (U.S. Government Printing
Office, Supt of Documents, Washington, DC 20402) No.1- 1972-
Volume(issue)/page/year: 45,119,1982
TYPE OF TEST : Sister chromatid exchange
TEST SYSTEM : Rodent - hamster Embryo
DOSE/DURATION : 25 mg/L
REFERENCE :
EVHPAZ EHP, Environmental Health Perspectives. (U.S. Government Printing
Office, Supt of Documents, Washington, DC 20402) No.1- 1972-
Volume(issue)/page/year: 45,119,1982
TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Rodent - hamster Lung
DOSE/DURATION : 750 mg/L
REFERENCE :
ESKGA2 Eisei Kagaku. Hygienic Chemistry. (Nippon Yakugakkai, 2-12-15
Shibuya, Shibuya-ku, Tokyo 150, Japan) V.1- 1953-
Volume(issue)/page/year: 28,P36,1982
*** STATUS IN U.S. ***
EPA GENETOX PROGRAM 1988, Inconclusive: CHO gene mutation
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JUNE 1998
*** END OF RECORD ***