• British Pharmacopoeia Volume IV
  • Appendices
  • Appendix I A. General Reagents

Fuchsin, Basic

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Fuchsin, Basic Basic violet 14; basic magenta; a mixture of rosaniline hydrochloride, C20H19N3,HCl (337.9) (CI 42510) and pararosaniline hydrochloride, C19H17N3,HCl (323.8) (CI 42500) (569-61-9)

A dark red powder or green crystals with a metallic lustre of such a purity that, when used in the preparation of decolorised fuchsin solution, an almost colourless solution is obtained. If necessary, purify in the following manner. Dissolve 1  g in 250  ml of 2m hydrochloric acid, allow to stand for 2 hours at room temperature, filter and neutralise with 2m sodium hydroxide and add 1  ml to 2  ml in excess. Filter the precipitate through a sintered-glass filter (40) and wash with water. Dissolve the precipitate in 70  ml of methanol, previously heated to boiling, and add 300  ml of water at 80°. Allow to cool to room temperature, filter and dry the crystals at a pressure of 2  kPa.

Store protected from light.